Ultrasound assisted enzymatic hydrolysis of sucrose catalyzed by invertase: Investigation on substrate, enzyme and kinetics parameters

Soares, Ariana de Souza; Augusto, Pedro Esteves Duarte; Júnior, Bruno Ricardo de Castro Leite; Nogueira, Camila; Vieira, Érica Nascif Rufino; Barros, Frederico Augusto Ribeiro de; Stringheta, Paulo César; Ramos, Afonso Mota

doi:10.1016/j.lwt.2019.02.083

Cited by 55 publications

(46 citation statements)

References 34 publications

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“…To determine the optimum pH and temperature of the enzyme, the AMG activity was performed at pH values of 3.5, 4.0, 4.5, 5.0 and 5.5, and at temperatures of 50, 55, 60, 65 and 70°C (in a water bath). Amyloglucosidase activity was determined according to Soares et al (2019) with some modifications. The reducing groups released by the enzyme action were determined using the 3,5-dinitrosalicyclic acid (DNS) method proposed by Miller (1959).…”

Section: Determination Of Amyloglucosidase Activity and Optimum Ph Anmentioning

confidence: 99%

“…at different temperatures and pHs. Then, all steps such as stop the reaction, spectrophotometer reading, standard curve construction were performed in the same way as Soares et al (2019).…”

Section: Determination Of Amyloglucosidase Activity and Optimum Ph Anmentioning

confidence: 99%

“…Several studies have already demonstrated the microbial inactivation capacity of ultrasound (Betts et al, 2014;Huang et al, 2017). Therefore, ultrasound could be used to ensure the microbiological quality of the commercial enzymatic solution during storage, without interfering in the efficiency of the reaction performed later (Soares et al, 2019). In fact, amyloglucosidase can be commercialized as solution, which facilitates its application but results in stability problems.…”

Section: Effect Of Ultrasonic Processing On Enzyme Activitymentioning

confidence: 99%

“…Although these preservatives have been accepted as safe, they have been avoided by the consumer, resulting in the need to develop new approaches to ensure the enzyme solution microbiological stability without using additives. Among several emerging technologies studied for this purpose, ultrasound technology can be an alternative (Soares et al, 2019).…”

Section: Effect Of Ultrasonic Processing On Enzyme Activitymentioning

confidence: 99%

“…Moreover, depending on the process conditions, ultrasound technology may not affect the enzymatic activity. In this case, the application of ultrasound aims to ensure the microbiological quality of the enzymatic solution, without interfering with the speed of the reaction (Soares et al, 2019), since several studies have demonstrated its ability to inactivate microorganisms (Betts, Williams, & Oakley, 2014;Huang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Ultrasound processing of amyloglucosidase: impact on enzyme activity, stability and possible industrial applications

et al. 2020

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This study evaluated the effect of ultrasound processing as a pre-treatment of amyloglucosidase on the enzymatic activity and stability. The activity was evaluated under optimal (65°C/ pH = 4.5) and non-optimal conditions of temperature and pH and its stability was evaluated during storage at 8°C. The enzyme solution was processed at 9.5 W L-1, 40 kHz, 23°C and at pH 3.5, 4.5, and 5.5, for up to 120 min. The activity was measured at 35, 65 and 80°C. The US process was able to increase, reduce or not alter the enzymatic activity, depending on the conditions applied. These modifications depended on the pH of the enzyme solution, the ultrasound processing time and the activity temperature. In different ultrasound conditions, mainly at 35 and 65°C, the enzyme activity did not change, demonstrating that this technology can be used for other purposes, such as microbial inactivation, without affecting the enzyme. The activity increase (up to 15%) occurred under non-optimal pH and temperature conditions (pH 3.5 or 5.5/ 80°C), suggesting that ultrasound promoted stabilization and enzymatic protection. This result is interesting in the starch saccharification, which requires the enzymatic reaction at high temperatures. Therefore, such results can increase the application range of this enzyme in different industrial applications.

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Section: Determination Of Amyloglucosidase Activity and Optimum Ph Anmentioning

confidence: 99%

“…at different temperatures and pHs. Then, all steps such as stop the reaction, spectrophotometer reading, standard curve construction were performed in the same way as Soares et al (2019).…”

Section: Determination Of Amyloglucosidase Activity and Optimum Ph Anmentioning

confidence: 99%

Section: Effect Of Ultrasonic Processing On Enzyme Activitymentioning

confidence: 99%

Section: Effect Of Ultrasonic Processing On Enzyme Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ultrasound processing of amyloglucosidase: impact on enzyme activity, stability and possible industrial applications

et al. 2020

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Intensified Nanoparticle Synthesis Using Hybrid Microwave and Ultrasound Treatments: Consecutive and Concurrent Modes

Shibatani

Kan

Asakuma

et al. 2020

Crystal Research and Technology

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Bubble formation around nucleated particle is inevitable when quick heating of microwave is employed in nanoparticle synthesis. Since these bubbles may cause bumping behavior, the bubble growth must be possibly suppressed to ensure stable microwave‐assisted operation. In this study, sequential and simultaneous ultrasound treatment and microwave irradiations on nanoparticle synthesis are proposed and investigated to assess the benefit of ultrasound in preventing superheating behavior and producing finer particles. Experimental results indicate that the inclusions of ultrasound treatment result in finer particle sizes and suppression of bumping behavior. Particularly, consecutive ultrasound treatment and microwave irradiation are recommendable due to its practicality and generating smallest bubbles and final particle sizes. This technique can therefore be expected as an innovative way to enhance industrial applications of quick thermal response induced by microwave.

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Chemical kinetics, thermodynamics and inactivation kinetics of dextransucrase activity by ultrasound treatment

Lan

Chen

2020

Reac Kinet Mech Cat

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Ultrasound assisted enzymatic hydrolysis of sucrose catalyzed by invertase: Investigation on substrate, enzyme and kinetics parameters

Cited by 55 publications

References 34 publications

Ultrasound processing of amyloglucosidase: impact on enzyme activity, stability and possible industrial applications

Ultrasound processing of amyloglucosidase: impact on enzyme activity, stability and possible industrial applications

Intensified Nanoparticle Synthesis Using Hybrid Microwave and Ultrasound Treatments: Consecutive and Concurrent Modes

Chemical kinetics, thermodynamics and inactivation kinetics of dextransucrase activity by ultrasound treatment

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