Ultrasound-assisted extraction and fast chromolithic method development, validation and system suitability analysis for 6, 8, 10-gingerols and shogaol in rhizome of Zingiber officinale by liquid chromatography-diode array detection

Gupta, AjaiPrakash; Gupta, Suphla; Datt, Prabhu; Pandotra, Pankaj; Sharma, Rajni; Kushwaha, Manoj

doi:10.4103/0973-8258.120197

Cited by 3 publications

(3 citation statements)

References 15 publications

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“…In the 8-gingerol compound, the tailing factor and asymmetry values have met the requirements, but the resolution value is still not eligible, namely RS ≥ 2. The results for method 3 are the retention times of all compounds that appear quite short, in the range of 6 to 8 minutes, which contradicts previous results (Gupta et al, 2013). The average for the results of the resolution values for all replications of method 3 with a value less than 2 means that it does not yet meet the resolution value requirements.…”

Section: Resultscontrasting

confidence: 71%

“…However, the retention time of method 4 is not the shortest among all 5 methods. However, it is still faster than previous results (Feng et al, 2014;Foudah et al, 2020;Gupta et al, 2013).…”

Section: Resultscontrasting

confidence: 61%

“…The tailing factors and asymmetry values are generally acceptable, suggesting symmetrical peak shapes. However, one limitation is that the retention times are similar to method 2, which may imply longer analysis time without significant improvement in separation (Foudah et al, 2020;Gupta et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

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Gingerol and shogaol on red ginger rhizome (Zingiber officinale var. Rubrum) using high-performance liquid chromatography

Sukweenadhi,

Damitasari,

Kartini

et al. 2023

Pharmaciana

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Red ginger (Zingiber officinale var. Rubrum) is one of the traditional medicines of the Zingiberaceae family, which contains phenolic ketone compounds, including gingerol and shogaol. At high temperatures, gingerol compounds become unstable and will change into shogaol. This study optimized conditions for the simultaneous separation of 6-gingerol, 8-gingerol, 10-gingerol and 6-shogaol from the red ginger extract using the High-Performance Liquid Chromatography method. The analytical conditions consisted of five different methods by modifying the composition of the mobile phase, the elution system, the flow rate of the mobile phase and the optimum UV wavelength. The best conditions for the simultaneous separation using a ratio of water: acetonitrile mobile phase in method 4 with a gradient elution system including 0 min (65:35); 1.5 min (40:60); 5-6.5 min (10:90); 7.5-9 min (0:100); 9.5-12 min (65:35); the mobile phase flow rate was 1.1 mL/min at an optimum wavelength of 230 nm with a retention time of 6-gingerol compound, 6-shogaol, 8-gingerol and 10-gingerol respectively were 4.947; 6.168; 6.554; and 7.412 min and its resolution were 2.267; 1.575; 1.315; and 2.215. Then the tailings factor and asymmetry values were obtained with an average value of ± 1.

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Section: Resultscontrasting

confidence: 71%

“…However, the retention time of method 4 is not the shortest among all 5 methods. However, it is still faster than previous results (Feng et al, 2014;Foudah et al, 2020;Gupta et al, 2013).…”

Section: Resultscontrasting

confidence: 61%

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Gingerol and shogaol on red ginger rhizome (Zingiber officinale var. Rubrum) using high-performance liquid chromatography

Sukweenadhi,

Damitasari,

Kartini

et al. 2023

Pharmaciana

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Simultaneous Determination of 6-Shogaol and 6-Gingerol in Various Ginger (Zingiber officinale Roscoe) Extracts and Commercial Formulations Using a Green RP-HPTLC-Densitometry Method

Foudah

Shakeel

Yusufoğlu

et al. 2020

Foods

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Various analytical methodologies have been reported for the determination of 6-shogaol (6-SHO) and 6-gingerol (6-GIN) in ginger extracts and commercial formulations. However, green analytical methods for the determination of 6-SHO and 6-GIN, either alone or in combination, have not yet been reported in literature. Hence, the present study was aimed to develop a rapid, simple, and cheaper green reversed phase high-performance thin-layer chromatography (RP-HPTLC) densitometry method for the simultaneous determination of 6-SHO and 6-GIN in the traditional and ultrasonication-assisted extracts of ginger rhizome, commercial ginger powder, commercial capsules, and commercial ginger teas. The simultaneous analysis of 6-SHO and 6-GIN was carried out via RP-18 silica gel 60 F254S HPTLC plates. The mixture of green solvents, i.e., ethanol:water (6.5:3.5 v/v) was utilized as a mobile phase for the simultaneous analysis of 6-SHO and 6-GIN. The analysis of 6-SHO and 6-GIN was performed at λmax = 200 nm for 6-SHO and 6-GIN. The densitograms of 6-SHO and 6-GIN from traditional and ultrasonication-assisted extracts of ginger rhizome, commercial ginger powder, commercial capsules, and commercial ginger teas were verified by obtaining their single band at Rf = 0.36 ± 0.01 for 6-SHO and Rf = 0.53 ± 0.01 for 6-GIN, compared to standard 6-SHO and 6-GIN. The green RP-HPTLC method was found to be linear, in the range of 100–700 ng/band with R2 = 0.9988 for 6-SHO and 50–600 ng/band with R2 = 0.9995 for 6-GIN. In addition, the method was recorded as “accurate, precise, robust and sensitive” for the simultaneous quantification of 6-SHO and 6-GIN in traditional and ultrasonication-assisted extracts of ginger rhizome, commercial ginger powder, commercial capsules, and commercial ginger teas. The amount of 6-SHO in traditional extracts of ginger rhizome, commercial ginger powder, commercial capsules, and commercial ginger teas was obtained as 12.1, 17.9, 10.5, and 9.6 mg/g of extract, respectively. However, the amount of 6-SHO in ultrasonication-assisted extracts of ginger rhizome, commercial ginger powder, commercial capsules, and commercial ginger teas were obtained as 14.6, 19.7, 11.6, and 10.7 mg/g of extract, respectively. The amount of 6-GIN in traditional extracts of ginger rhizome, commercial ginger powder, commercial capsules, and commercial ginger teas were found as 10.2, 15.1, 7.3, and 6.9 mg/g of extract, respectively. However, the amount of 6-GIN in ultrasonication-assisted extracts of ginger rhizome, commercial ginger powder, commercial capsules, and commercial ginger teas were obtained as 12.7, 17.8, 8.8, and 7.9 mg/g of extract, respectively. Overall, the results of this study indicated that the proposed analytical technique could be effectively used for the simultaneous quantification of 6-SHO and 6-GIN in a wide range of plant extracts and commercial formulations.

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Silica-Based Monolithic Columns as a Tool in HPLC—An Overview of Application in Analysis of Active Compounds in Biological Samples

et al. 2020

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Monolithic fillings used in chromatography are of great interest among scientists since the first reports of their synthesis and use were published. In the 20 years since silica-based monolithic columns were introduced into the commercial market, numerous papers describing their chromatographical properties and utility in various branches of industry and scientific investigations were presented. This review is focused on possible applications of commercially available silica-based HPLC monolithic columns in the analysis of biological samples.

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Ultrasound-assisted extraction and fast chromolithic method development, validation and system suitability analysis for 6, 8, 10-gingerols and shogaol in rhizome of Zingiber officinale by liquid chromatography-diode array detection

Cited by 3 publications

References 15 publications

Gingerol and shogaol on red ginger rhizome (Zingiber officinale var. Rubrum) using high-performance liquid chromatography

Gingerol and shogaol on red ginger rhizome (Zingiber officinale var. Rubrum) using high-performance liquid chromatography

Simultaneous Determination of 6-Shogaol and 6-Gingerol in Various Ginger (Zingiber officinale Roscoe) Extracts and Commercial Formulations Using a Green RP-HPTLC-Densitometry Method

Silica-Based Monolithic Columns as a Tool in HPLC—An Overview of Application in Analysis of Active Compounds in Biological Samples

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