Ultrasound-assisted extraction of alantolactone and isoalantolactone from Inula helenium roots

Трендафилова, Антоанета; Chanev, Christo; Todorova, Milka

doi:10.4103/0973-1296.66942

Cited by 47 publications

(34 citation statements)

References 9 publications

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“…Recently, UAE had been widely for extraction of sesquiterpene,[5] flavonoids,[6] phenolics,[7] and polysaccharide,[8] etc.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound-assisted extraction of total flavonoids from Inula helenium

Jin¹,

Zhao²,

Guo³

et al. 2012

Phcog Mag

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Background:Inula helenium was a perennial herb belonging to composite family and the roots of I. helenium have been widely used in traditional Chinese medicine. In this study, I. helenium was used as an experimental matrix.Materials and Methods:Ultrasound-assisted extraction (UAE) of total flavonoids from I. helenium was studied with dual wavelength UV-VIS spectrophotometer. Effects of various factors including ratio of material to liquid, ultrasonic time, ethanol concentration and extraction times on extraction yield of total flavonoids were evaluated. Then, orthogonal design of four factors at three levels was applied for optimization the extraction yields of flavonoids from the root of I. helenium.Result:The optimal extracting process of the total flavonoids from the root of the I. helenium was 1 g plant sample with 20 ml of 60% ethanol, extracting twice and each time for 20 min.Conclusion:Under these optimal conditions, the yield of total flavonoids was (17.36±0.94) mg/g. UAE was more efficient and time saving for the extraction of flavonoids from plant materials.

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“…Recently, UAE had been widely for extraction of sesquiterpene,[5] flavonoids,[6] phenolics,[7] and polysaccharide,[8] etc.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound-assisted extraction of total flavonoids from Inula helenium

Jin¹,

Zhao²,

Guo³

et al. 2012

Phcog Mag

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“…EASP extract was mainly composed of an enhances solvent penetration through plant cell with the aid of sound wave. 9 Its mechanistic action includes fragmentation, erosion, and destruction-detexturation of plant structures, thus, this will enhance the diffusion process by increasing the solute transfer rate. 10,11 This method is usually employed for its higher extraction efficiency and its shorter extraction time as compared to maceration and soaking with the less amount of solvent application.…”

Section: Gc-ms Analysis For Ethyl Acetate Extract Of S Polyanthum Lementioning

confidence: 99%

“…10,11 This method is usually employed for its higher extraction efficiency and its shorter extraction time as compared to maceration and soaking with the less amount of solvent application. 9,10 Recently, UAE method was applied in many researches to enhance extractability of phenolic, thermo-labile and unstable compounds. 12,13 A recent study by Ramli et al 13 has utilized this method for extraction of S. polyanthum leaves, and has proven that the extract possess significant renal protective effect in Spontaneously Hypertensive Rats.…”

Section: Gc-ms Analysis For Ethyl Acetate Extract Of S Polyanthum Lementioning

confidence: 99%

GC-MS Analysis of Phytochemical Compounds in Syzygium polyanthum Leaves Extracted using Ultrasound-Assisted Method

Rahim¹,

Ismail²,

Omar³

et al. 2017

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Context:Syzygium polyanthum has been traditionally formulated by the folklore for the treatment of diseases including diarrhea, rheumatism, diabetes mellitus, hypercholesterolemia, hypertension, gastritis and hyperuricemia. Normally, its phytochemicals are always extracted using solvent, maceration and steam distillation methods, but the use of ultrasound-assisted extraction (UAE) method is still not well documented. Aims: This study aims to extract the phytochemical compounds present in S. polyanthum leaves using UAE and to identify them by Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Methodology: The leaves were consecutively soaked with n-hexane, ethyl acetate and methanol in a bath sonicator to derive n-hexane (HSP), ethyl acetate (EASP), and methanol (MSP) extracts of S. polyanthum leaves and then the extracts were subjected to GC-MS analysis. Mass-spectral databases of peaks were compared with database from Wiley, NIST and FNSCC libraries for compound identification. Results: GC-MS analyses of HSP, EASP and MSP showed the presence of 21, 27, and 31 peaks, respectively. The major compound for HSP (31.912%), EASP (27.042%), and MSP (22.386%) were unknown compounds which were detected at retention time between 61.980 and 62.29 min, thus requires further characterization. Squalene and phytol were among the other major compounds present in all three extracts. Several identified compounds in the extracts such as squalene, phytol, hentriacontane, palmitic acid, α-pinene, nerolidol, linalool, α-tocopherol and β-tocopherol were known bioactive compounds. analysis of crude methanolic S. polyanthum leaves extract showed the presence of tannins, flavonoids, glycosides, alkaloids, carbohydrates, steroids, triterpenoids and flavonoids. 5,6 Phenolic compounds such as caffeic acid and gallic acid were previously identified in crude macerated methanolic extract of S. polyanthum leaves by high performance liquid chromatography and liquid chromatography mass spectrometry analyses. Conclusion: GC-MS analyses of n-hexane (HSP), ethyl acetate (EASP),7 Analysis on the crude macerated methanolic extract of S. polyanthum leaves has detected squalene as the major compound. 5,8 Another analysis on the macerated n-hexane extract similarly found squalene as the major compound (30.45%) followed by n-hentriacontane (6.57%). 8Most phytochemicals previously studied were extracted using solvent maceration and steam distillation methods, however, the use of ultrasound-assisted extraction (UAE) method is not well-documented. The present study has utilized UAE method, a different extraction method from the other previous studies. Basically, UAE method Rahim et al.: GC-MS Analysis of Syzygium polyanthum Leaves Extracted using Ultrasound-Assisted Extraction MethodPharmacognosy Journal, Vol 10, Issue 1, Jan-Feb, 2018 111 GC conditionThe injector temperature was set at 250 º C, column temperature program was set at 50ºC (0 min) with an increasing rate of 3 º C/min to 300 º C (10 min). The carrier gas used was pure helium gas (9...

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“…Alantolactone is a traditional Chinese medicinal herb officially listed in pharmacopeias (Stojakowska et al, 2006;Trendafilova et al, 2010). It belongs to a sesquiterpene lactone, and mainly extracted from the roots of I. helenium L. Alantolactone possesses an inhibitory activity for inflammation, microbial and cell growth in several cancer cell lines (Konishi et al, 2002;Lawrence et al, 2001) as reported before.…”

Section: Discussionmentioning

confidence: 82%

Mechanism-based inhibition of Alantolactone on human cytochrome P450 3A4 in vitro and activity of hepatic cytochrome P450 in mice

Qin

et al. 2015

Journal of Ethnopharmacology

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Ultrasound-assisted extraction of alantolactone and isoalantolactone from Inula helenium roots

Cited by 47 publications

References 9 publications

Ultrasound-assisted extraction of total flavonoids from Inula helenium

Ultrasound-assisted extraction of total flavonoids from Inula helenium

GC-MS Analysis of Phytochemical Compounds in Syzygium polyanthum Leaves Extracted using Ultrasound-Assisted Method

Mechanism-based inhibition of Alantolactone on human cytochrome P450 3A4 in vitro and activity of hepatic cytochrome P450 in mice

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