Ultrasound-Assisted Extraction of Bioactive Compounds from Annatto Seeds, Evaluation of Their Antimicrobial and Antioxidant Activity, and Identification of Main Compounds by LC/ESI-MS Analysis

Abstract

This study evaluated the antimicrobial activity (i.e., against Bacillus cereus and Staphylococcus aureus) and the antioxidant activity (i.e., ABTS, FRAP, and DPPH) of annatto seeds extract obtained by ultrasound-assisted extraction. A response surface design with three levels such as pH (2-11), solvent concentration (50-96 %), seed-to-solvent ratio (1:2–1:10), and treatment time (0-30 min) was employed to determine the optimal experimental conditions. Thus, a pH of 7.0, seed-to-solvent ratio of 1:7, and treatment time of 20 min were selected as optimal rendering an extract having a 0.62% of bixin, 3.81 mg gallic acid/mg equivalent of polyphenol compounds (ABTS 1035.7, FRAP 424.7, and DPPH 1161.5 μM trolox/L), and a minimal inhibitory concentration against Bacillus cereus and Staphylococcus aureus of 32 and 16 mg/L, respectively. Further, the main bioactive compounds identified by LC/ESI-MS were bixin and catechin, chlorogenic acid, chrysin, butein, hypolaetin, licochalcone A, and xanthohumol.

Shifting of Physicochemical and Biological Characteristics of Coffee Roasting Under Ultrasound-Assisted Extraction

“…However, the process leads to the loss of phenolic compounds due to high temperature and long extraction time periods ( 14 ). Ultrasound-assisted extraction (UAE) is an efficient method for the extraction of natural antioxidants from plant materials ( 15 ) because it reduces the time required for extraction and energy expenditure and enhances the yield of the bioactive compounds ( 16 ). In view of the great advantages possessed by UAE, the current study has been designed in a way to examine the pH, °Brix, antioxidant activities, polyphenol content, and phenolic compounds following the integration of UAE during the coffee roasting process.…”

Use of Plant Proteins as Microencapsulating Agents of Bioactive Compounds Extracted from Annatto Seeds (Bixa orellana L.)

“…In fact, these extracts have been used in food matrices such as dairy, meat, and baked products, mostly due to their coloring property [1,2]. The antioxidant and antimicrobial activities of the extract are attributed to the bioactive compounds found in the seeds, such as the bixin carotenoid (bixin or 6-methyl hydrogen (9Z)-6,6 -diapocarotene-6) and polyphenol compounds such as catechin, chlorogenic acid, chrysin, butein, hypoaletin, and xanthoangelol [3]. These compounds inhibit the production of free radicals and denature the proteins present in the cellular structure of some microorganisms to prevent their proliferation [1,[4][5][6].…”

Techno-economic and uncertainty analyses of heat- and ultrasound-assisted extraction technologies for the production of crude anthocyanins powder from Hibiscus sabdariffa calyx

“…Industrially, HAE-t also has some advantages which include, ease of scalability, equipment availability and low equipment and maintenance costs. Other modern technologies of enhancing bioactive extraction from plant materials are ultrasounds (Quintero Quiroz et al, 2019), microwaves (Proestos & Komaitis, 2008), pulsed electric fields (Nowacka et al, 2019) and pressurized and supercritical fluids (Rodríguez-Meizoso et al, 2008). However, among the modern technologies, ultrasound-assisted extraction technology (UAE-t) stands out because of its numerous advantages which include low solvent consumption, low energy consumption, low extraction time and high extraction yield (Chemat et al, 2017).…”