Ultrasound-assisted extraction (UAE) was applied to extract total antioxidants from two rapeseed varieties. The antioxidant capacity of spring and winter rapeseed cultivars was determined by two different analytical methods: 2,2âČ-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2âČ-diphenyl-1-picrylhydrazyl (DPPH). Effects of three independent variables, sonication time (t), volume of methanol-torapeseed weight ratio (V/m), and concentration of methanol (c) on the antioxidant capacity of rapeseed varieties, were studied by response surface methodology (RSM). Box-Behnken design (BBD) was used to obtain the optimal conditions of the UAE. The optimal UAE conditions were evaluated with an extraction time of 13.8 min, a solvent-to-material ratio of 26.2 mL/g and a solvent concentration of 50.3 % for antioxidant capacity of spring rapeseed cultivar (ABTS=62.7 mmol Trolox equivalent (TE)/100 g, DPPH=38.3 mmol TE/100 g), whereas the predicted optimum t=13.3 min, V/m=29.5 mL/g, and c=38.3 % resulted in ABTS=62.0 mmol TE/100 g, DPPH=48.6 mmol TE/100 g for a winter rapeseed cultivar. Results of ABTS and DPPH determinations correlate with the predicted values (R 2 =0.9435 and 0.9796 for the spring rapeseed variety and 0.9952, 0.9252 for the winter rapeseed variety).