Ultrasound-assisted extraction of polysaccharide from spent Lentinus edodes substrate: Process optimization, precipitation, structural characterization and antioxidant activity

Wang, Kaijie; Guo, Juntong; Cheng, Junxia; Zhao, Xinghua; Ma, Bohan; Yang, Xingbin; Shao, Hongjun

doi:10.1016/j.ijbiomac.2021.09.174

Cited by 49 publications

(29 citation statements)

References 40 publications

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“…The unique β-glycosidic linkage absorption peak at 887 cm -1 implied the existence of β-glucans in the SLPs (Mathlouthi M Fau -Koenig & Koenig). Furthermore, the weak bands of ring deformation and ring stretching from α-D-(1-6) and α-D-(1-4) linkages were near 768 cm -1 (Wang et al, 2019b), which was consistent with the results of polysaccharides from spent Lentinus edodes substrate (Wang et al, 2021a). According to the these findings, we concluded that the SLP fractions were heteropolysaccharides composed of characteristic groups of hydroxyl, alkyl, ester, β-glucans, and D-pyran and furan rings.…”

Section: Uv-vis Spectra and Ft-ir Analysis Of Slpssupporting

confidence: 88%

Preparation, characterization, and bioactivities of polysaccharides fractions from sugarcane leaves

JIANG

CHEN

et al. 2023

Food Sci. Technol

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Three new polysaccharides (SLP-50, SLP-70, and SLP-90) were fractionated successively by gradient concentrations of ethanol. This study aimed to investigate the initial structural characterization and bioactivities of these sugarcane leaves polysaccharides (SLPs) obtained by ultrasound-assisted extraction. The results, which were further validated by IR spectrometry, revealed that the three SLPs contained uronic acids, proteins, and sulfate groups in addition to carbohydrate. SLP-50 (36.40 kDa), SLP-70 (12.97 kDa), and SLP-90 (3.52 kDa) were acidic heteropolysaccharides mainly comprised of mannose (Man), rhamnose (Rha), glucuronic acid (Glc A), galacturonic acid (Gal A), galactose (Gal), glucose (Glc), xylose (Xyl), and arabinose (Ara), in different molar ratios. SLP-90 exhibited higher antioxidant capacity than SLP-50 and SLP-70. In vitro, all fractions showed significant hypoglycemic potential and antibacterial activity against Gram-positive and Gram-negative bacteria. The IC 50 values of SLP-50, SLP-70, and SLP-90 were 0.11, 0.05, and 0.67 mg/mL on α-glucosidase, respectively, which were significantly lower than that of acarbose (0.80 mg/mL). These findings could provide a reference for developing and applying SLP-based functional foods.

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Section: Uv-vis Spectra and Ft-ir Analysis Of Slpssupporting

confidence: 88%

Preparation, characterization, and bioactivities of polysaccharides fractions from sugarcane leaves

JIANG

CHEN

et al. 2023

Food Sci. Technol

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“…Contrastingly, UAE-AX exhibited more stacked and "worm-like" formations. The AKE-AX maintained more regular structures in traditional extraction process, while the UAE-AX chains were found conjugating together to form irregular structures with rough surfaces due to the destruction of the rigid structures and the improvement of chain exibility, which was consistent with the results obtained by Wang et al (2021b). Such changes were mainly caused by the disruption of hydrogen bonds by the shearing and cavitation effects of ultrasound.…”

Section: Morphologysupporting

confidence: 86%

“…Ultrasound-assisted extraction (UAE) was a novel green extraction technique. The application of ultrasonic irradiation during extraction could effectively extract polysaccharides from various materials, such as mushrooms (Wang et al 2021b), sun ower (Ezzati et al 2020), and okra (Chen et al 2021b). During sonication, vacuum bubbles were generated and released large amounts of energy when they collapsed, promoting the transportation of compounds and breaking covalent bonds.…”

Section: Introductionmentioning

confidence: 99%

Modified structural and functional properties of arabinoxylan derived from brewers’ spent grain by ultrasound-assisted extraction

Liu

Chen

Coldea

et al. 2022

Preprint

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Ultrasound-assisted extraction (UAE) was used to obtain arabinoxylan (AX) from brewers’ spent grain (BSG) with a higher yield, modified structural characteristics, and improved techno-functional properties. Results showed that an UAE procedure working at 400 W for 15 min under a 60% duty cycle could increase the AX yield by 52.41% compared with a traditional alkaline extraction without sonication. Concomitantly, UAE-AX developed a degraded Mw of 2741 kDa, a changed conformation of less molecular asymmetry and a decreased mean particle size from 12.56 µm to 4.993 µm. The rheological properties of UAE-AX aqueous solution were improved, leading to higher viscosity and gel-forming capacity of the AX stabilized emulsions. As a result, UAE-AX addition could reduce the mean droplet size to 6.40 µm and better stabilize the emulsion under various conditions than traditional alkaline extracted AX. Thus, the results proved UAE to be a promising method of extracting AX from BSG with valuable utilization in food processing.

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“…Wang et al. (2021) also used gradient ethanol precipitation to separate spent L entinus edodes substrate polysaccharides (SP) into SP40, SP60, and SP80, where SP80 had the highest total and reducing sugar content, SP40 contained the highest protein content, and SP60 showed the highest uric acid content. Therefore, it is crucial to control the ethanol concentration during the extraction of polysaccharides.…”

Section: Resultsmentioning

confidence: 99%

“…Fernando et al (2020) extracted the fucoidan fractions (SSQC1-SSQC4) of Sargassum siliquastrum by step gradient ethanol precipitation, where SSQC1 showed the highest yield and the total sugar content decreased with increasing ethanol concentration, while the sulfate and fucose content were the opposite. Wang et al (2021) also used gradient ethanol precipitation to separate spent Lentinus edodes substrate polysaccharides (SP) into SP40, SP60, and SP80, where SP80 had the highest total and reducing sugar content, SP40 contained the highest protein content, and SP60 showed the highest uric acid content. Therefore, it is crucial to control the ethanol concentration during the extraction of polysaccharides.…”

Section: Extraction Yield and Chemical Composition Of Vl8-epssmentioning

confidence: 99%

Structural characterization and immunomodulatory effects of extracellular polysaccharide from Lactobacillus paracasei VL8 obtained by gradient ethanol precipitation

Liu

Mao

Zhang

et al. 2022

Journal of Food Science

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In this study, gradient ethanol precipitation method was applied to obtain the extracellular polysaccharides of Lactobacillus paracasei VL8 (VL8‐EPS). The yields, physicochemical properties, and immunomodulatory effects of VL8‐EPS obtained by precipitation at different ethanol concentrations (30%, 50%, and 70%, v/v) were compared. The results showed that VL8‐EPSs were high molecular weight sulfated heteropolysaccharides, composed mainly of glucose and galactose, and the alteration of ethanol concentration had an effect on their chemical compositions, molecular weight distributions, monosaccharide composition, and surface structure, while the primary structure remained the same. Among the three polysaccharide fractions, VL8‐EPS50 displayed better immunomodulatory activities compared with VL8‐EPS30 and VL8‐EPS70. VL8‐EPS50 was found to exert immunomodulatory effects by enhancing the phagocytic activity of RAW264.7 cells and to promote their secretion of more nitric oxide; it also showed stronger thermal and solution stability. In summary, there was a correlation between the structural characteristics of polysaccharides and their immunomodulatory activity, and VL8‐EPS50 was preferentially used for in vivo immunomodulatory activity. Practical ApplicationThis study opens up the source of raw materials for functional foods, which can provide some theoretical basis for the research and development of extracellular polysaccharides of lactic acid bacteria and promote their application in the future development of food industry.

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Ultrasound-assisted extraction of polysaccharide from spent Lentinus edodes substrate: Process optimization, precipitation, structural characterization and antioxidant activity

Cited by 49 publications

References 40 publications

Preparation, characterization, and bioactivities of polysaccharides fractions from sugarcane leaves

Preparation, characterization, and bioactivities of polysaccharides fractions from sugarcane leaves

Modified structural and functional properties of arabinoxylan derived from brewers’ spent grain by ultrasound-assisted extraction

Structural characterization and immunomodulatory effects of extracellular polysaccharide from Lactobacillus paracasei VL8 obtained by gradient ethanol precipitation

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