Vitis vinifera L., commonly known as Muscat grapes is an aromatic food product which has a beneficial effect on the human body due to its phytonutrient potential. Studies have reported that grapes have antiobesity, antidiabetic, antiinflammatory, anticarcinogenic, cardioprotective, vasorelaxant, phytoestrogenic, and neuroprotective properties. This study aimed to identify the phytonutrients such as alkaloids, flavonoids, phenol, steroid, tannin, and terpenoids of the coloured seeded grapes. Whole grapes were collected during the vegetation period and divided into three groups as whole fruit (W), fruit skin (P) and seed (S). All three samples were freeze-dried and soaked with the aqueous solution. Ultrasonic homogenized material was used for extraction. Further analysis is done in FTIR for qualitative measurement of the main functional group and antioxidant activity was determined through the DPPH method. The findings revealed that Muscat grapes may be considered as a natural rich source of phenolic amalgam with a notable amount of antioxidant capacity.