Ultrasound-Assisted Extraction of Syringin from the Bark of Ilex rotunda Thumb Using Response Surface Methodology

Zhao, Lichun; He, Ying; Deng, Xin; Xia, Xiang-Hua; Liang, Jian; Yang, Gengliang; Li, Wei; Wang, Hui

doi:10.3390/ijms13067607

Cited by 20 publications

(8 citation statements)

References 24 publications

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“…Compared with a one-factor-at-a-time design, which is adopted most frequently in the literature, the fractional factorial experimental design is more efficient in reducing the number of experimental runs and time for investigating the optimal conditions of wax esters synthesis. To date, response surface methodology (RSM) has been applied successfully for modeling various biochemical processes, such as enzyme immobilization [ 18 , 19 ], extraction [ 20 , 21 ], fermentation [ 22 ] and biofuel production [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

High Yield of Wax Ester Synthesized from Cetyl Alcohol and Octanoic Acid by Lipozyme RMIM and Novozym 435

Kuo

Chen

et al. 2012

IJMS

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Wax esters are long-chain esters that have been widely applied in premium lubricants, parting agents, antifoaming agents and cosmetics. In this study, the biocatalytic preparation of a specific wax ester, cetyl octanoate, is performed in n-hexane using two commercial immobilized lipases, i.e., Lipozyme® RMIM (Rhizomucor miehei) and Novozym® 435 (Candida antarctica). Response surface methodology (RSM) and 5-level-4-factor central composite rotatable design (CCRD) are employed to evaluate the effects of reaction time (1–5 h), reaction temperature (45–65 °C), substrate molar ratio (1–3:1), and enzyme amount (10%–50%) on the yield of cetyl octanoate. Using RSM to optimize the reaction, the maximum yields reached 94% and 98% using Lipozyme® RMIM and Novozym® 435, respectively. The optimum conditions for synthesis of cetyl octanoate by both lipases are established and compared. Novozym® 435 proves to be a more efficient biocatalyst than Lipozyme® RMIM.

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Section: Introductionmentioning

confidence: 99%

High Yield of Wax Ester Synthesized from Cetyl Alcohol and Octanoic Acid by Lipozyme RMIM and Novozym 435

Kuo

Chen

et al. 2012

IJMS

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“…The behaviour obtained indicates that, due to the extraction time prolongs the chemical decomposition of mangiferin may occur. Even if ultrasound-assisted extraction has considerable potential in extraction fields of natural constituents, composition of some edible fruits is deteriorated after ultrasound treatment (Zhao et al, 2012). Therefore, it is importante to research and to examine the mechanism of ultrasonic degradation of mangiferin and other compounds presents in Mangifera indica.…”

Section: Optimization the Mangiferin Extraction Using A Rsmmentioning

confidence: 99%

APPLICATION OF ULTRASOUND ASSISTED EXTRACTION IN THE MANGIFERIN OBTAINING FROM Mangifera indica L LEAVES

Sevilla¹,

Salomon²,

Ayala³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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-Mangiferin is a natural xantone extensively distributed in higher plants showing different pharmacological properties. In the present study, an ultrasonicassisted extraction (UAE) method was evaluated for obtain mangiferin from Mangifera indica L. (c.v Mango Macho) leaves. Differents extraction parameters were optimized using a response surface methodology and the best conditions reached were at 47% ethanol concentration, a liquid-to-solid ratio 47:1, and an extraction time of 63min. under ultrasound irradiation of 150W. In these circumstances, the yield of mangiferin was 24.15±0.015mg/g. For first time, was evaluated the ultrasound assited extraction to higher frecuency and the value of the yield reached was 6.38± 0.062mg/g. Comparison of UAE of mangiferin respect to microwave and aggited tank was realized and the yield obtained by UAE was inferior, however the ultrasonic time was minor respect to conventional extraction technique. The ultrasonic-assisted extraction is an alternative method to use for the extraction of mangiferin from Cuban mangos.

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“…The bark of Ilex rotunda Thunb is used for the treatment of colds, tonsillitis, sore throat, acute gastroenteritis, and dysentery in China [15,16]. The main bioactive constituents of I. rotunda are triterpenoid saponins, phenolic acid compounds, and flavonoids.…”

Section: Introductionmentioning

confidence: 99%

Rotundarpene inhibits TNF-α-induced activation of the Akt, mTOR, and NF-κB pathways, and the JNK and p38 associated with production of reactive oxygen species

et al. 2017

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Ilex Rotunda Thunb has been shown to have anti-inflammatory and antioxidant effects. In human keratinocytes, we investigated the effect of rotundarpene (4-caffeoyl-3-methyl-but-2-ene-1,4-diol) on the TNF-α-stimulated production of inflammatory mediators in relation to the Akt, mTOR, and NF-κB pathways, and the JNK and p38-MAPK. Rotundarpene, Akt inhibitor, Bay 11-7085, rapamycin, and N-acetylcysteine inhibited the TNF-α-stimulated production of cytokines and chemokines, increase in the levels of p-Akt and mTOR, activation of NF-κB, and production of reactive oxygen species in keratinocytes. TNF-α treatment induced phosphorylation of the JNK and p38-MAPK. Inhibitors of the c-JNK (SP600125) and p38-MAPK (SB203580) reduced the TNF-α-induced production of inflammatory mediators, binding of NF-κB to DNA, and activation of the JNK and p38-MAPK in keratinocytes. The results show that rotundarpene may reduce the TNF-α-stimulated inflammatory mediator production by suppressing the reactive oxygen species-dependent activation of the Akt, mTOR, and NF-κB pathways, and activation of the JNK and p38-MAPK in human keratinocytes. Additionally, rotundarpene appears to attenuate the Akt, mTOR, and NF-κB pathways and the JNK and p38-MAPK-mediated inflammatory skin diseases.

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Ultrasound-Assisted Extraction of Syringin from the Bark of Ilex rotunda Thumb Using Response Surface Methodology

Cited by 20 publications

References 24 publications

High Yield of Wax Ester Synthesized from Cetyl Alcohol and Octanoic Acid by Lipozyme RMIM and Novozym 435

High Yield of Wax Ester Synthesized from Cetyl Alcohol and Octanoic Acid by Lipozyme RMIM and Novozym 435

APPLICATION OF ULTRASOUND ASSISTED EXTRACTION IN THE MANGIFERIN OBTAINING FROM Mangifera indica L LEAVES

Rotundarpene inhibits TNF-α-induced activation of the Akt, mTOR, and NF-κB pathways, and the JNK and p38 associated with production of reactive oxygen species

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