Ultrasound-assisted one-phase solvent extraction coupled with liquid chromatography-quadrupole time-of-flight mass spectrometry for efficient profiling of egg yolk lipids

Xie, Yunfei; Wu, Bangfu; Wu, Zhixian; Tu, Xinghao; Xu, Shuling; Lv, Xiongwen; Yin, Huajun; Xiang, Jingjing; Chen, Hong; Wei, Fang

doi:10.1016/j.foodchem.2020.126547

Cited by 50 publications

(44 citation statements)

References 37 publications

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“…Lipid composition was measured by MetWare (Wuhan, China) following the standard protocol 44 . For lipidomics analysis, 220 mg dried endosperm powder was extracted with 3 mL of 2-propanol for 1 h and centrifuged (3000 g, 5 min).…”

Section: Methodsmentioning

confidence: 99%

Carotenoids modulate kernel texture in maize by influencing amyloplast envelope integrity

et al. 2020

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The mechanism that creates vitreous endosperm in the mature maize kernel is poorly understood. We identified Vitreous endosperm 1 (Ven1) as a major QTL influencing this process. Ven1 encodes β-carotene hydroxylase 3, an enzyme that modulates carotenoid composition in the amyloplast envelope. The A619 inbred contains a nonfunctional Ven1 allele, leading to a decrease in polar and an increase in non-polar carotenoids in the amyloplast. Coincidently, the stability of amyloplast membranes is increased during kernel desiccation. The lipid composition in endosperm cells in A619 is altered, giving rise to a persistent amyloplast envelope. These changes impede the gathering of protein bodies and prevent them from interacting with starch grains, creating air spaces that cause an opaque kernel phenotype. Genetic modifiers were identified that alter the effect of Ven1A619, while maintaining a high β-carotene level. These studies provide insight for breeding vitreous kernel varieties and high vitamin A content in maize.

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Section: Methodsmentioning

confidence: 99%

Carotenoids modulate kernel texture in maize by influencing amyloplast envelope integrity

et al. 2020

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“…(2019a) identified 1,180 lipid species in pork samples collected from black pigs in different places. Tibetan pork contained 28 higher lipid species (14 GL, 8 GP, 2 SL, 2 FA, and 2 PR) and Jilin pork contained 18 higher lipid species (4 GL, 4 GP, 3 ST, 3 UPLC-Q-TOF MS 57 molecular species of phospholipids (Ali et al, 2017) Chicken egg yolks High-resolution MS Glycerophosphocholines and glycerophosphoethanolamines (Wood et al, 2021) Chicken egg yolks LC-ESI-Q-TOF MS 618 lipid species, Tibetan and docosahexaenoic acid egg showed higher phospholipid and lower TG (Xie et al, 2020) Chicken egg (male and female ) (Garcia et al, 2012) Goat, soy, and bovine milk UPLC-Q-Exactive Orbitrap MS Goat milk was rich in medium-chain TG, USFA, and n-6 and n-3 FA, soy milk was rich in PC, PE, PS, and PG, while bovine milk was rich in TG, DG, and Cer, fourteen lipids potential markers (Li et al, 2017) Cow milk (heat stress of cow) UHPLC-Q-TOF MS Heat stress increased long-chain fatty acids; moreover, reduced PE, PS, PC, LPC and glucoceramide, potential markers: LPC (Liu et al, 2017) UPLC, ultrahigh performance liquid chromatography; ESI, electrospray ionization; MS, mass spectrometry; IMF, intramuscular fat; LPCs, lysophosphatidylcholines; Q-TOF, quadrupole-time-of-flight; LC-MS, liquid chromatography-MS; LPEs, lysophosphatidylethanolamines; FAC, FA carnitines.…”

Section: Lipidomics For Meat Quality Evaluationmentioning

confidence: 99%

“…Ceramide phosphoethanolamine, a naturally occurring lipid, is a key membrane component ( Masood et al, 2010 ), whereas hydroxysphingomyelin plays an essential role in reducing the risk of prostate cancer and endometrial cancer ( Li et al., 2017 ; Schmidt et al, 2020 ). Xie et al (2020) used ultrasound-assisted one-phase solvent extraction method coupled with LC-ESI-Q-TOF MS for profiling of egg yolk lipids; 618 lipid species were identified in the yolks of green shell eggs, Tibetan eggs, native eggs, and docosahexaenoic acid eggs. Moreover, PCA can clearly distinguish the four types of eggs: Tibetan and docosahexaenoic acid egg yolks showed higher PL content and lower TG content.…”

Section: Application Of Lipidomics To Assess Food From Farm Animalsmentioning

confidence: 99%

“…Lipidomics has been successfully applied to assess food obtained from animals; for example, it has been used for the characterization and discrimination of domestic pig ( Mi et al, 2019a ) and Taihe black-boned silky fowl muscles ( Mi et al, 2018 ). Further, Li et al (2020b) used quantitative lipidomics to reveal alterations in donkey milk lipids during lactation, and Xie et al (2020) used it for the efficient profiling of egg yolk lipids.…”

Section: Introductionmentioning

confidence: 99%

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A Comprehensive Review of Lipidomics and Its Application to Assess Food Obtained from Farm Animals

Song¹,

Cai²,

Song³

et al. 2022

Food Sci Anim Resour

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Lipids are one of the major macronutrients essential for adequate growth and maintenance of human health. Their structure is not only complex but also diverse, which makes systematic and holistic analyses challenging; consequently, little is known regarding the relationship between phenotype and mechanism of action. In recent years, rapid advancements have been made in the fields of lipidomics and bioinformatics. In comparison with traditional approaches, mass spectrometry-based lipidomics can rapidly identify as well as quantify >1,000 lipid species at the same time, facilitating comprehensive, robust analyses of lipids in tissues, cells, and body fluids. Accordingly, lipidomics is now being widely applied in various fields, particularly food and nutrition science. In this review, we discuss lipid classification, extraction techniques, and detection and analysis using lipidomics. We also cover how lipidomics is being used to assess food obtained from livestock and poultry. The information included herein should serve as a reference to determine how to characterize lipids in animal food samples, enhancing our understanding of the application of lipidomics in the field in animal husbandry.

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“…So a nutritional strategy to improve the human diets in those nutrients is Eggs are an inexpensive food used in human nutrition all over the world and are considered an important source of high-quality protein, minerals and vitamins (Kishimoto et al 2016). The nutritional value of eggs is partially attributed to polyunsaturated fatty acid (PUFA) content in yolk (Xie et al 2020) but also to other bioactive lipophilic compounds such as vitamins or carotenoids, the yolk being a lipidic carrier of antioxi-Supported by the Romanian Ministry of Research, Innovation and Digitalization (Project No. 8 PCCDI 043 PC3).…”

mentioning

confidence: 99%

Study on the influence of dietary sea buckthorn meal on nutritional properties of laying hen eggs

Untea¹,

Panaite²,

Varzaru³

et al. 2021

CZECH J ANIM SCI

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The study aimed to evaluate the transfer of bioactive compounds from sea buckthorn meal (SBM) supplement to eggs and the effect on lipid quality and peroxidation process. Thirty-two-week-old TETRA SL laying hens were allocated to two dietary treatments, 30 birds each (15 replicate pens). The control diet (C) contained a maize-soybean diet, and the experimental diet contained the previous C diet with 2% of maize replaced with SBM. Thirty-six eggs from each group were collected in order to determine the quality parameters at the end of the experiment. The antioxidant profile of SBM showed that the phytoadditive is a valuable source of vitamin E, xanthophylls and polyphenols. The inclusion of 2% of SBM in the experimental diet led to a more than 25% increase of vitamin E and an almost 50% increase of xanthophylls compared to the control. The markers specific to the coronary risk decreased significantly in the experimental group compared to the control, showing a beneficial effect of dietary SBM on the quality of yolk lipids. The bioactive compounds detected in egg yolk showed a significant (P < 0.05) improvement of the antioxidant profile, the rate of vitamin (A and E), carotenoid (lutein and zeaxanthin) and mineral (iron and zinc) deposition increased after dietary SBM inclusion. Regarding the lipid peroxidation parameters, the dietary SBM inclusion acted in the first phase of the lipid peroxidation process by inhibiting the formation of primary oxidation products (hydroperoxides and conjugated dienes). In conclusion, the sea buckthorn supplementation of the laying hen diet improved the bioactive compound concentrations in eggs and delayed the oxidation process of yolk during storage.

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Ultrasound-assisted one-phase solvent extraction coupled with liquid chromatography-quadrupole time-of-flight mass spectrometry for efficient profiling of egg yolk lipids

Cited by 50 publications

References 37 publications

Carotenoids modulate kernel texture in maize by influencing amyloplast envelope integrity

Carotenoids modulate kernel texture in maize by influencing amyloplast envelope integrity

A Comprehensive Review of Lipidomics and Its Application to Assess Food Obtained from Farm Animals

Study on the influence of dietary sea buckthorn meal on nutritional properties of laying hen eggs

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