Ultrasound-enhanced catalytic hairpin assembly capable of ultrasensitive microRNA biosensing for the early screening of Alzheimer's disease

Luo, Yong; Chen, Jingyu; Liang, Jiahui; Liu, Yizhen; Liu, Conghui; Liu, Yibiao; Xu, Tailin; Zhang, Xueji

doi:10.1016/j.bios.2023.115746

Cited by 6 publications

(2 citation statements)

References 45 publications

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“…The use of BTV xMAP detection allows for rapid assessment of BTV epidemiology and may provide information for decision-making related to control and prevention measures. Yong Luo et al [ 98 ] developed an ultrasound-enhanced catalytic hairpin assembly (UECHA) biosensing platform for effective biomarker enrichment and fluorescence enhancement in the early screening of Alzheimer’s disease. By introducing a portable acoustic driving platform and functionalized microspheres, the platform concentrates the functionalized microspheres in the center of the microcavity, accompanied by enhanced fluorescence signals with specific release.…”

Section: Applications Of Fluorescent Microspheres In Multiplex Nuclei...mentioning

confidence: 99%

Recent Advances in and Application of Fluorescent Microspheres for Multiple Nucleic Acid Detection

Chen,

Luo,

Ren

et al. 2024

Biosensors

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Traditional single nucleic acid assays can only detect one target while multiple nucleic acid assays can detect multiple targets simultaneously, providing comprehensive and accurate information. Fluorescent microspheres in multiplexed nucleic acid detection offer high sensitivity, specificity, multiplexing, flexibility, and scalability advantages, enabling precise, real-time results and supporting clinical diagnosis and research. However, multiplexed assays face challenges like complexity, costs, and sample handling issues. The review explores the recent advancements and applications of fluorescent microspheres in multiple nucleic acid detection. It discusses the versatility of fluorescent microspheres in various fields, such as disease diagnosis, drug screening, and personalized medicine. The review highlights the possibility of adjusting the performance of fluorescent microspheres by modifying concentrations and carrier forms, allowing for tailored applications. It emphasizes the potential of fluorescent microsphere technology in revolutionizing nucleic acid detection and advancing health, disease treatment, and medical research.

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Section: Applications Of Fluorescent Microspheres In Multiplex Nuclei...mentioning

confidence: 99%

Recent Advances in and Application of Fluorescent Microspheres for Multiple Nucleic Acid Detection

Chen,

Luo,

Ren

et al. 2024

Biosensors

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“…Thus, acoustic-based micro/nanomotors have been widely applied in the field of biomedicine. For example, Yong Luo and others introduced a portable acousticdriven platform with functionalized microspheres for effective biomarker enrichment and fluorescence enhancement [34]. Integrating capillary technology with ultrasound to induce biomarker enrichment in nano-liter samples [35].…”

Section: Introductionmentioning

confidence: 99%

Controlling the Collective Behaviors of Ultrasound-Driven Nanomotors via Frequency Regulation

Zhao,

Chen,

Zhan

et al. 2024

Micromachines

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Controlling the collective behavior of micro/nanomotors with ultrasound may enable new functionality in robotics, medicine, and other engineering disciplines. Currently, various collective behaviors of nanomotors, such as assembly, reconfiguration, and disassembly, have been explored by using acoustic fields with a fixed frequency, while regulating their collective behaviors by varying the ultrasound frequency still remains challenging. In this work, we designed an ultrasound manipulation methodology that allows nanomotors to exhibit different collective behaviors by regulating the applied ultrasound frequency. The experimental results and FEM simulations demonstrate that the secondary ultrasonic waves produced from the edge of the sample cell lead to the formation of complex acoustic pressure fields and microfluidic patterns, which causes these collective behaviors. This work has important implications for the design of artificial actuated nanomotors and optimize their performances.

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Integrated Ultrasound‐Enrichment and Machine Learning in Colorimetric Lateral Flow Assay for Accurate and Sensitive Clinical Alzheimer's Biomarker Diagnosis

Wang,

Zhu,

Zhou

et al. 2024

Advanced Science

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The colloidal gold nanoparticle (AuNP)‐based colorimetric lateral flow assay (LFA) is one of the most promising analytical tools for point‐of‐care disease diagnosis. However, the low sensitivity and insufficient accuracy still limit its clinical application. In this work, a machine learning (ML)‐optimized colorimetric LFA with ultrasound enrichment is developed to achieve the sensitive and accurate detection of tau proteins for early screening of Alzheimer's disease (AD). The LFA device is integrated with a portable ultrasonic actuator to rapidly enrich microparticles using ultrasound, which is essential for sample pre‐enrichment to improve the sensitivity, followed by ML algorithms to classify and predict the enhanced colorimetric signals. The results of the undiluted serum sample testing show that the protocol enables efficient classification and accurate quantification of the AD biomarker tau protein concentration with an average classification accuracy of 98.11% and an average prediction accuracy of 99.99%, achieving a limit of detection (LOD) as sensitive as 10.30 pg mL−1. Further point‐of‐care testing (POCT) of human plasma samples demonstrates the potential use of LFA in clinical trials. Such a reliable lateral flow immunosensor with high precision and superb sensing performance is expected to put LFA in perspective as an AD clinical diagnostic platform.

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Ultrasound-enhanced catalytic hairpin assembly capable of ultrasensitive microRNA biosensing for the early screening of Alzheimer's disease

Cited by 6 publications

References 45 publications

Recent Advances in and Application of Fluorescent Microspheres for Multiple Nucleic Acid Detection

Recent Advances in and Application of Fluorescent Microspheres for Multiple Nucleic Acid Detection

Controlling the Collective Behaviors of Ultrasound-Driven Nanomotors via Frequency Regulation

Integrated Ultrasound‐Enrichment and Machine Learning in Colorimetric Lateral Flow Assay for Accurate and Sensitive Clinical Alzheimer's Biomarker Diagnosis

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