Ultrasound enhanced detection of individual meningococcal serogroups by latex immunoassay

Sobański, M.; Vince, Rebecca V.; Biagini, Giancarlo A.; Cousins, Caroline M.; Guiver, Malcolm; Gray, Steve; Kaczmarski, Edward B.; Coakley, W. Terence

doi:10.1136/jcp.55.1.37

Cited by 18 publications

(11 citation statements)

References 17 publications

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“…Although they provide more rapid results than culture or PCR, and can give positive results even after a few days of treatment, while culture or PCR cannot, much controversy has arisen over their proper use and variable performance [12][13][14]. More recently, the changing epidemiology of meningococcal disease compelled recommendation of kits capable of identifying meningococcal serogroup W135.…”

Section: The Latmentioning

confidence: 99%

Biological diagnosis of meningococcal meningitis in the African meningitis belt: Current epidemic strategy and new perspectives

Chanteau

Rose²,

Djibo

et al. 2007

Vaccine

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Section: The Latmentioning

confidence: 99%

Biological diagnosis of meningococcal meningitis in the African meningitis belt: Current epidemic strategy and new perspectives

Chanteau

Rose²,

Djibo

et al. 2007

Vaccine

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“…Conventional serological classification by a simple bacterial agglutination test can occasionally be difficult because of specificity problems associated with grouping antisera, downregulation of N. meningitidis capsule expression, the propensity of certain strains to autoagglutinate or the propensity of these bacteria to exchange DNA by transformation (Yakubu et al, 1999;Tzanakaki et al, 2001). Ultrasound-enhanced latex agglutination (LA), standard PCR and fluorescent PCR methods have been used to confirm the presence of meningococcal antigens or DNA in clinical samples (Jenkins et al, 1997;Guiver et al, 2000;Diggle et al, 2001;Pollard et al, 2002;Sobanski et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Direct-test PCR for detection of meningococcal DNA and its serogroup characterization: standardization and adaptation for use in a public health laboratory

Baethgen¹,

Moraes²,

Weidlich³

et al. 2003

Journal of Medical Microbiology

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A direct PCR test (DT-PCR) was established to detect Neisseria meningitidis DNA in clinical samples from patients with suspected bacterial meningitis. Specific primers for the 16S rDNA of N. meningitidis were designed to amplify a 600 bp DNA fragment. One hundred and ninety-three clinical samples were analysed, corresponding to 114 samples from patients diagnosed as positive and 79 as negative for infection by N. meningitidis using conventional methods (culture, latex agglutination and counterimmunoelectrophoresis). These samples were submitted to PCR by two different clinical sample preparation approaches (with and without DNA extraction and purification) and submitted to different PCR protocols to improve the results. In agarose gel detection, the sensitivity value for DT-PCR was 88·5 % and, using dot-blot DNA detection, the sensitivity increased to 96·4 %. The detection limit for meningococcus in cerebrospinal fluid was 2310 2 c.f.u. ml À1 .Serogroup prediction was done using a multiplex PCR protocol and the sensitivity was 83 % for agarose gel DNA detection and 96·4 % using dot-blot DNA detection.

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“…One of the approaches for circumventing the limitations of the conventional particle agglutination assay, and particularly shortening the time of analysis and increasing the assay sensitivity, is the use of ultrasound (Ellis & Sobanski, 2000) (Sobanski et al, 2002) (Wiklund & Hertz, 2006). This ultrasonic method is based on the fact that ultrasonic forces increase the probability of bead collisions required for antigen-antibody cross-linking.…”

Section: Introductionmentioning

confidence: 99%

A rapid ultrasound particle agglutination method for HIV antibody detection: Comparison with conventional rapid HIV tests

Bystryak

Ossina

2017

Journal of Virological Methods

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We present the results of the feasibility and preliminary studies on analytical performance of a rapid test for detection of human immunodeficiency virus (HIV) antibodies in human serum or plasma that is an important advance in detecting HIV infection. Current methods for rapid testing of antibodies against HIV are qualitative and exhibit poor sensitivity (limit of detection). In this paper, we describe an ultrasound particle agglutination (UPA) method that leads to a significant increase of the sensitivity of conventional latex agglutination tests for HIV antibody detection in human serum or plasma. The UPA method is based on the use of: 1) a dual mode ultrasound, wherein a first single-frequency mode is used to accelerate the latex agglutination process, and then a second swept-frequency mode of sonication is used to disintegrate non-specifically bound aggregates; and 2) a numerical assessment of results of the agglutination process. The numerical assessment is carried out by optical detection and analysis of moving patterns in the resonator cell during the swept-frequency mode. The single-step UPA method is rapid and more sensitive than the three commercial rapid HIV test kits analyzed in the study: analytical sensitivity of the new UPA method was found to be 510-, 115-, and 80-fold higher than that for Capillus™, Multispot™ and Uni-Gold™ Recombigen HIV antibody rapid test kits, respectively. The newly developed UPA method opens up additional possibilities for detection of a number of clinically significant markers in point-of-care settings.

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Ultrasound enhanced detection of individual meningococcal serogroups by latex immunoassay

Cited by 18 publications

References 17 publications

Biological diagnosis of meningococcal meningitis in the African meningitis belt: Current epidemic strategy and new perspectives

Biological diagnosis of meningococcal meningitis in the African meningitis belt: Current epidemic strategy and new perspectives

Direct-test PCR for detection of meningococcal DNA and its serogroup characterization: standardization and adaptation for use in a public health laboratory

A rapid ultrasound particle agglutination method for HIV antibody detection: Comparison with conventional rapid HIV tests

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