Ultrasound enhancement of cationic lipid-mediated gene transfer to primary tumors following systemic administration

Anwer, Khursheed; Kao, G. Y.; Proctor, Belinda; Anscombe, I; Florack, Valarie J.; Earls, R; Wilson, Elizabeth M.; McCreery, Thomas P.; Unger, Evan C.; Rolland, Alain; Sullivan, Sean

doi:10.1038/sj.gt.3301302

Cited by 136 publications

(66 citation statements)

References 31 publications

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“…Lipoplexes in combination with US can enhance transgene expression by several orders of magnitude. 110,111 Manome et al reported that US of 20 W/cm 2 , at continuous 1 MHz, increased b-galactosidase expression by 3-270-fold, in vitro and in vivo. 112 In the presence of microbubbles, US enhanced gene expression up to 300-fold over plasmid alone.…”

Section: Microbubbles and Ultrasoundmentioning

confidence: 99%

Non-viral gene delivery in skeletal muscle: a protein factory

Lu¹,

Bou‐Gharios²,

Partridge³

2003

Gene Ther

170

109

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Ever since the publication of the first reports in 1990 using skeletal muscle as a direct target for expressing foreign transgenes, an avalanche of papers has identified a variety of proteins that can be synthesized and correctly processed by skeletal muscle. The impetus to the development of such applications is not only amelioration of muscle diseases, but also a range of therapeutic applications, from immunization to delivery of therapeutic proteins, such as clotting factors and hormones. Although the most efficient way of introducing transgenes into muscle fibres has been by a variety of recombinant viral vectors, there are potential benefits in the use of non-viral vectors. In this review we assess the recent advances in construction and delivery of naked plasmid DNA to skeletal muscle and highlight the options available for further improvements to raise efficiency to therapeutic levels.

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Section: Microbubbles and Ultrasoundmentioning

confidence: 99%

Non-viral gene delivery in skeletal muscle: a protein factory

Lu¹,

Bou‐Gharios²,

Partridge³

2003

Gene Ther

170

109

View full text Add to dashboard Cite

show abstract

“…[3][4][5][6][7][8][9][10][11][12][13][14][15] Anwer et al 15 reported that application of ultrasound to skin tumours before or after the injection of lipoplexes resulted in a significant increase in gene transfer specifically to the tumour. 15 The magnitude of increased expression ranged from 3-4 to 270-fold. Similarly, ultrasound in the presence of microbubbles enhanced gene expression by up to 300-fold over naked DNA transfection.…”

Section: Introductionmentioning

confidence: 99%

Microbubble ultrasound improves the efficiency of gene transduction in skeletal muscle in vivo with reduced tissue damage

et al. 2003

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“…25 Moreover, after systemic administration, ultrasound has been reported to enhance cationic lipidmediated gene transfer to primary tumors. 28 A variety of ultrasound sources with respect to focusing, waveform, and intensity level have been applied to augment transgene expression. Focused sinusoidal ultrasound, as studied in the present work, may achieve the best proportion of transfection and cell viability, 19 since the effects of this type of ultrasound can be focused on a defined target area, while sparing the surrounding tissue.…”

Section: Discussionmentioning

confidence: 99%

“…These gaseous contrast bubbles had been shown to enhance ultrasound-mediated transfection in vitro via increased cavitation. [28][29][30][31][32] Both the treated carotid arteries and the contralateral carotids were harvested 48 h after sonication. Up to three vessel segments, 0.5 cm in length, containing areas of sonication, along with the segment that contained DNA infusion only, were immediately frozen in liquid nitrogen.…”

Section: Animal Model and Transfection Conditionmentioning

confidence: 99%

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Focused ultrasound (HIFU) induces localized enhancement of reporter gene expression in rabbit carotid artery

et al. 2003

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The development of accurate, safe, and efficient gene delivery remains a major challenge towards the realization of gene therapeutic prevention and treatment of cardiovascular diseases. In this study, we investigated the ability of high-intensity focused ultrasound (HIFU), a form of mechanical wave transmission, to act as a noninvasive tool for the enhancement of in vivo gene transfer into rabbit carotid arteries. Segments of the common carotid arteries of New Zealand white rabbits were isolated and infused with plasmid DNA encoding the reporter b-galactosidase either with or without the addition of ultrasound contrast agent consisting of small (B2-5 mm) gasfilled human albumin microspheres to augment cavitation. Infused arteries were exposed to pulsed ultrasound for 1 min (frequency 0.85 MHz, burst length 50 ms, repetition frequency 1 Hz, duration 60 s, peak pressure amplitude of 15 MPa). At 6.3 MPa, HIFU enhanced gene expression eight-fold, and 17.5-fold in the presence of contrast. We found increasing amounts of b-galactosidase expression in the carotid vessel with increasing pressure amplitude. This dose-response relation was present with and without contrast. Without contrast, no vessel damage was detected up to 15 MPa, while the addition of contrast induced side effects above a threshold of 6.3 MPa peak pressure. The entire procedure was feasible and safe for the animals, and the results suggest that HIFU has the potential to assist in the noninvasive spatial regulation of gene transfer into the vascular system.

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Ultrasound enhancement of cationic lipid-mediated gene transfer to primary tumors following systemic administration

Cited by 136 publications

References 31 publications

Non-viral gene delivery in skeletal muscle: a protein factory

Non-viral gene delivery in skeletal muscle: a protein factory

Microbubble ultrasound improves the efficiency of gene transduction in skeletal muscle in vivo with reduced tissue damage

Focused ultrasound (HIFU) induces localized enhancement of reporter gene expression in rabbit carotid artery

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