Ultrasound-induced and MRI-monitored CuO nanoparticles release from micelle encapsulation

Abstract

Copper oxide nanoparticles (CuO NPs) have anticancer and antimicrobial activities. Moreover, they have a contrast enhancing effect in both MRI and ultrasound. Nonetheless, encapsulation is needed to control their toxic side effects and a mechanism for release on demand is required. A methodology is introduced herein for encapsulating and releasing CuO NPs from micelles by ultrasound induced hyperthermia and monitoring the process by MRI. For this aim, CuO NPs loaded poly(ethylene glycol)-block-poly(D,L-lactic acid) (PEG-b-PLA) micelles were prepared. Then, the profile of copper release with application of ultrasound was examined as a function of time and temperature using a colorimetric method. Finally, T1 weighted MRI images of suspensions and ex vivo poultry liver samples containing the CuO NPs loaded micelles were acquired before and after ultrasound application. The results confirmed that: (i) encapsulated NPs are detectible by MRI T1 mapping, depicting substantial T1 shortening from 1872 ± 62 ms to 683 ± 20 ms. (ii) Ultrasonic hyperthermia stimulated the NPs release with an about threefold increase compared to non-treated samples. (iii) Releasing effect was clearly visible by T1-weighted imaging (mean signal increase ratio of 2.29). These findings can potentially lead to the development of a new noninvasive methodology for CuO NPs based theranostic process.

Fiber Optic SPR Sensor Modified With Copper Oxide Nanoparticles for Highly Sensitive and Selective Detection of Dopamine

“…The resulting pellet was re-dispersed in water and the colloidal solution of CuO NPs was kept at room temperature for further use. The copper concentration of the samples was determined by a previously described polyethyleneimine (PEI) colorimetric method using UV-Visible spectrophotometric measurement at 275 nm (Ultrospec 2100 pro UV/vis spectrophotometer, Biochrom Ltd, UK) [38], [39]. Morphology of the particles was assessed by transmission electron microscopy (Tecnai T12 G2 TEM, FEI Technologies Inc, Oregon, USA), and size distribution was determined by dynamic light scattering (DLS) (ZetaSizer Nano ZS, Malvern Panalytical, UK), Fig.…”

Emerging applications of nanotechnology in context to immunology: A comprehensive review

“…It is considered an emerging, sensitive and successful technology for the detection of bacteria, viruses, and cancer cells at an early stage ( Mocan et al, 2017 ). • Some CuO NPs are used in the noninvasive methodology-based theranostic process because they possess a contrast-enhancing effect in Magnetic resonance imaging and ultrasound ( Aresteanu et al, 2020 ; Zeng et al, 2020 ). …”

“…• Some CuO NPs are used in the noninvasive methodology-based theranostic process because they possess a contrast-enhancing effect in Magnetic resonance imaging and ultrasound ( Aresteanu et al, 2020 ; Zeng et al, 2020 ).…”