Ultrasound meets the cell membrane: for enhanced endocytosis and drug delivery

Abstract

Endocytosis plays crucial role during drug delivery for precision therapy. As a non-invasive and spatiotemporal-controllable stimulus, ultrasound (US) has been utilized for improving drug delivery efficiency due to its ability...

Structurally Dynamic Monocyte–Liposome Hybrid Vesicles as an Anticancer Drug Delivery Vehicle: A Crucial Correlation of Microscopic Elasticity and Ultrafast Dynamics

“…35 Among these mechanisms, receptor-mediated endocytosis is well-documented. 37 This process involves the creation of pits on the cell surface through specific receptors, enabling cellular uptake. 35 In our study, the presence of monocytes in the Monocyte-LP-Dox system might facilitate their binding to the A549 cell surface.…”

“…39 Despite numerous efforts to enhance delivery efficacy by directing systems toward the endocytosis pathway using external stimuli such as light, electric or magnetic fields, and ultrasound, their practical application is often hindered by tissue complexity and the risk of tissue damage due to uncontrolled application. 37 In contrast, the improved endocytosis efficacy of the hybrid (Monocyte-LP) to A549 cells during Dox delivery is primarily attributed to the inherent modification through monocyte fusion without any external stimuli of the liposomal bilayer. The next step involves unravelling the intriguing factors associated with Monocyte-LP interactions that confer enhanced efficacy, such a feature is totally absent in the parental systems.…”

“…While the possibility of membrane fusion-mediated internalization cannot be disregarded, Stella et al have proposed endocytosis as the primary internalization pathway, particularly in systems with a prevalence of PC-based liposomal compositions . Despite numerous efforts to enhance delivery efficacy by directing systems toward the endocytosis pathway using external stimuli such as light, electric or magnetic fields, and ultrasound, their practical application is often hindered by tissue complexity and the risk of tissue damage due to uncontrolled application . In contrast, the improved endocytosis efficacy of the hybrid (Monocyte-LP) to A549 cells during Dox delivery is primarily attributed to the inherent modification through monocyte fusion without any external stimuli of the liposomal bilayer.…”

“…In drug delivery systems, the effectiveness of delivery is significantly influenced by various factors, with the internalization mechanism of recipient cells and subsequent intracellular trafficking playing a pivotal role . Among these mechanisms, receptor-mediated endocytosis is well-documented . This process involves the creation of pits on the cell surface through specific receptors, enabling cellular uptake .…”

The Effect of a Magnetic Field on the Transport of Functionalized Magnetite Nanoparticles into Yeast Cells

“…The manner of nanoparticle internalization depends on its size and shape, surface charge, and topography. An important feature is surface functionalization [12,49]. Depending on the size, endocytosis is divided into two types: phagocytosis, during which the uptake of large particles (>500 nm) occurs, and pinocytosis, which includes different mechanisms such as macropinocitosis, clathrin/caveolin-mediated endocytosis, and clathrin/caveolin independent endocytosis.…”