Ultrasound modulation of bioluminescence generated inside a turbid medium

Ahmad, Jaleel; Jayet, Baptiste; Hill, Philip J.; Mather, Melissa L.; Dehghani, Hamid; Morgan, Stephen P.

doi:10.1117/12.2251349

Cited by 2 publications

(5 citation statements)

References 6 publications

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“…FUS-enhanced CL have also been studied ex vivo on dissected tissues and outcomes have been promising. Ahmad et al (2017) observed clear peaks of two chemiluminescent sources (encapsulated inside plastic tubes) placed at 10 mm distance in chicken breast tissue at depth of 20 mm. Meanwhile, the experiments of Kobayashi et al (2016) were even more interesting that they reached resoluble signals at a depth of 25 mm (Figure 8) in porcine tissues.…”

Section: Fus-cl In Ex Vivo Studiesmentioning

confidence: 91%

“…In order to mimic tissue environments, agarose phantom is usually used as it has similar scattering coefficient as that of native tissues following Monte Carlo model (Wang et al, 1995). Since native tissues may vary from species to species, phantoms typically have a scattering coefficient from 1 to 80 cm −1 (Kobayashi et al, 2016;Ahmad et al, 2017;Zhu et al, 2018). Through phantoms, transparent silicon tubes were run at different depths.…”

Section: Fus-cl In Turbid Microenvironmentsmentioning

confidence: 99%

“…Using FUS at different stimulation levels, authors not only observed 10 times higher signal-to-noise ratio (Zhu et al, 2018) for 1064 nm luminescent laser but also increased sensitivity or better spatial resolution. The best resolution was reported at 2 mm (Ahmad et al, 2017;Klein et al, 2018) using 640 nm laser or 18 F lasers. The deepest penetration were obtained at 25-30 mm (Kobayashi et al, 2016) recorded on agarose phantom using POCL system.…”

Section: Fus-cl In Turbid Microenvironmentsmentioning

confidence: 99%

“…In most cases in literature, we can see that the more powerful the FUS, the greater the penetration and resolution. For instance, once FUS was increased from 1 to 2 MHz, signals were 10 times stronger as normalized to background (Klein et al, 2018;Zhu et al, 2018) while increasing FUS from 2 to 3 MHz allowed fourfold deeper penetration and 33% better resolution (Ahmad et al, 2017;Zhu et al, 2018).…”

Section: Fus-cl In Turbid Microenvironmentsmentioning

confidence: 99%

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Ultrasound-Enhanced Chemiluminescence for Bioimaging

Dhamecha

Gonsalves

et al. 2020

Front. Bioeng. Biotechnol.

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Tissue imaging has emerged as an important aspect of theragnosis. It is essential not only to evaluate the degree of the disease and thus provide appropriate treatments, but also to monitor the delivery of administered drugs and the subsequent recovery of target tissues. Several techniques including magnetic resonance imaging (MRI), computational tomography (CT), acoustic tomography (AT), biofluorescence (BF) and chemiluminescence (CL), have been developed to reconstruct three-dimensional images of tissues. While imaging has been achieved with adequate spatial resolution for shallow depths, challenges still remain for imaging deep tissues. Energy loss is usually observed when using a magnetic field or traditional ultrasound (US), which leads to a need for more powerful energy input. This may subsequently result in tissue damage. CT requires exposure to radiation and a high dose of contrast agent to be administered for imaging. The BF technique, meanwhile, is affected by strong scattering of light and autofluorescence of tissues. The CL is a more selective and sensitive method as stable luminophores are produced from physiochemical reactions, e.g. with reactive oxygen species. Development of near infrared-emitting luminophores also bring potential for application of CL in deep tissues and whole animal studies. However, traditional CL imaging requires an enhancer to increase the intensity of low-level light emissions, while reducing the scattering of emitted light through turbid tissue environment. There has been interest in the use of focused ultrasound (FUS), which can allow acoustic waves to propagate within tissues and modulate chemiluminescence signals. While light scattering is decreased, the spatial resolution is increased with the assistance of US. In this review, chemiluminescence detection in deep tissues with assistance of FUS will be highlighted to discuss its potential in deep tissue imaging.

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Section: Fus-cl In Ex Vivo Studiesmentioning

confidence: 91%

Section: Fus-cl In Turbid Microenvironmentsmentioning

confidence: 99%

Section: Fus-cl In Turbid Microenvironmentsmentioning

confidence: 99%

Section: Fus-cl In Turbid Microenvironmentsmentioning

confidence: 99%

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Ultrasound-Enhanced Chemiluminescence for Bioimaging

Dhamecha

Gonsalves

et al. 2020

Front. Bioeng. Biotechnol.

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“…Three mechanisms result in light modulation by ultrasound in scattering medium: first is the variation of optical properties caused by ultrasound compression and rarefaction; the second involves modification of optical phase due to displacement of scatterers; and finally, modulation of optical phase due to changes in the refractive index. While the first mechanism is the only one affected by non-coherent light, the last two mechanisms appear to be affected by coherent light [2][3][4] . This paper experimentally studies tagging efficiency using detection of amplitude metrics on coherent versus non-coherent light as an illumination source in AOS.…”

Section: Introductionmentioning

confidence: 99%

Acousto-optic sensing: tagging efficiency in long coherent laser vs. laser diode and LED

Omidali,

Rahayu,

Zhao

et al. 2023

Advanced Optical Imaging Technologies VI

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Diffusing light modulated by focused ultrasound (FUS) or acousto optic (AO) sensing is a hybrid technique that utilizes ultrasound features, such as, deep penetration, localize and precise spatial resolution, to surpass optical scattering limitations in optical sensing and maintain the original optical contrast. In this paper, we compare the acousto-optic (AO) signal amplitude when using three different light sources; long coherence, laser diode (LD), and light emitting diode (LED). In recent years, there has been a growing trend towards the use of LEDs and laser diode LDs in optical applications because of their compact and small size, ease of use, safety features, and cost-effectiveness. Aim of this paper is to examine the capability of LDs and LEDs to be used in AO sensing. We evaluated differences in the tagging efficiency using detection of AO signal amplitude metrics. The results showed that particularly LDs are also capable of providing acceptable tagging efficiency in AO-based sensing compared with long coherent lasers and can be beneficial option for use in AO based techniques.

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Ultrasound modulation of bioluminescence generated inside a turbid medium

Cited by 2 publications

References 6 publications

Ultrasound-Enhanced Chemiluminescence for Bioimaging

Ultrasound-Enhanced Chemiluminescence for Bioimaging

Acousto-optic sensing: tagging efficiency in long coherent laser vs. laser diode and LED

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