Ultrasound Stimulation Induces PGE2 Synthesis Promoting Cementoblastic Differentiation Through EP2/EP4 Receptor Pathway

Braga, Emanuel; Inubushi, Toshihiro; Kawazoe, Aki; Tanimoto, Kotaro; Miyauchi, Mutsumi; Tanaka, Eiji; Takata, Takashi; Tanne, Kazuo

doi:10.1016/j.ultrasmedbio.2010.03.008

Cited by 28 publications

(20 citation statements)

References 43 publications

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“…Among various types of biophysical stimuli, the three individual types of stimuli selected for this study have been proven to have positive effects on osteogenic potential. [12][13][14][15][16] We hypothesized that combining these biophysical stimuli could be a more powerful method for enhancing osteogenic potential induced by each individual stimulus synergistically. 17 The developed bioreactor was able to generate and transfer both individual and combined biophysical signals using three different stimuli to cells on the 3D scaffolds.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, an electromagnetic field and ultrasound are nonbiomimetic stimuli frequently used for treatment of bone fracture. Even though these stimuli are effective for enhancing osteogenic differentiation and bone formation, [12][13][14][15][16] there are still only a few articles reporting the effects of combining two or more stimuli.…”

Section: Introductionmentioning

confidence: 99%

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Combined Effect of Three Types of Biophysical Stimuli for Bone Regeneration

Kang

Hong²,

Jeong³

et al. 2014

Tissue Engineering Part A

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Combined Effect of Three Types of Biophysical Stimuli for Bone Regeneration

Kang

Hong²,

Jeong³

et al. 2014

Tissue Engineering Part A

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“…The mechanism for the induction effects of ultrasound on the gene expression of fungal enzyme is still unclear. It was reported that ultrasound mediates the metabolism of mouse cells through mitogen-activated protein kinases (MAPKs) pathway [40]. MAPKs have also been characterized in different plant and fungi and appear to function coordinately to regulate the transcription factors and the expression of specific sets of genes in response to environmental stress [41,42].…”

Section: Effect Of the Ultrasonic Treatments On The Expression Of Lccmentioning

confidence: 99%

Ultrasound-intensified laccase production from Trametes versicolor

Wang

Chen

et al. 2013

Ultrasonics Sonochemistry

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a b s t r a c tAn efficient intermittent ultrasonic treatment strategy was developed to improve laccase production from Trametes versicolor mycelia cultures. The optimized strategy consisted of exposing 2-day-old mycelia cultures to 5-min ultrasonic treatments for two times with a 12-h interval at the fixed ultrasonic power and frequency (120 W, 40 kHz). After 5 days of culture, this strategy produced the highest extracellular laccase activity of 588.9 U/L among all treatments tested which was 1.8-fold greater than the control without ultrasound treatment. The ultrasonic treatment resulted in a higher pellet porosity that facilitated the mass transfer of nutrients and metabolites from the pellets to the surrounding liquid. Furthermore, the ultrasonic treatment induced the expression of the laccase gene (lcc), which correlated with a sharp increase in both extracellular and intracellular laccase activity. This is the first study to find positive effects of ultrasound on gene expression in fungal cells. These results provide a basis for understanding the stimulation of metabolite production and process intensification by ultrasonic treatment in filamentous fungal culture.

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“…In this context, the combination of stem cell types and the use of differentiating stimuli (chemical or physical) are important for obtaining the best results [10,11,12]. …”

Section: Introductionmentioning

confidence: 99%

Non-Ionizing Radiation for Cardiac Human Amniotic Mesenchymal Stromal Cell Commitment: A Physical Strategy in Regenerative Medicine

Ledda

D’Emilia

Lolli

et al. 2018

IJMS

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Cell therapy is an innovative strategy for tissue repair, since adult stem cells could have limited regenerative ability as in the case of myocardial damage. This leads to a local contractile dysfunction due to scar formation. For these reasons, refining strategy approaches for “in vitro” stem cell commitment, preparatory to the “in vivo” stem cell differentiation, is imperative. In this work, we isolated and characterized at molecular and cellular level, human Amniotic Mesenchymal Stromal Cells (hAMSCs) and exposed them to a physical Extremely Low Frequency Electromagnetic Field (ELF-EMF) stimulus and to a chemical Nitric Oxide treatment. Physically exposed cells showed a decrease of cell proliferation and no change in metabolic activity, cell vitality and apoptotic rate. An increase in the mRNA expression of cardiac and angiogenic differentiation markers, confirmed at the translational level, was also highlighted in exposed cells. Our data, for the first time, provide evidence that physical ELF-EMF stimulus (7 Hz, 2.5 µT), similarly to the chemical treatment, is able to trigger hAMSC cardiac commitment. More importantly, we also observed that only the physical stimulus is able to induce both types of commitments contemporarily (cardiac and angiogenic), suggesting its potential use to obtain a better regenerative response in cell-therapy protocols.

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Ultrasound Stimulation Induces PGE2 Synthesis Promoting Cementoblastic Differentiation Through EP2/EP4 Receptor Pathway

Cited by 28 publications

References 43 publications

Combined Effect of Three Types of Biophysical Stimuli for Bone Regeneration

Combined Effect of Three Types of Biophysical Stimuli for Bone Regeneration

Ultrasound-intensified laccase production from Trametes versicolor

Non-Ionizing Radiation for Cardiac Human Amniotic Mesenchymal Stromal Cell Commitment: A Physical Strategy in Regenerative Medicine

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