Ultrasound-synergized electrostatic field extraction of total flavonoids from Hemerocallis citrina baroni

Abstract

The total flavonoids from Hemerocallis citrina baroni are regarded as a green and natural health care product with many beneficial impacts on human health. In this study, ultrasound-synergized electrostatic field extraction (UEE) of the total flavonoids (TF) from H. citrina was investigated. Significant independent variables of the extraction, including the electrostatic field, ultrasonic power, ethanol concentration and extraction time, were optimized using the Box-Behnken (BB) method, and the optimal extraction conditions were obtained by response surface methodology (RSM). The extraction yield using UEE was compared with the yields obtained using only ultrasound extraction (UE) and water bath extraction (WE), using a UV-vis spectrophotometer. The best extraction yield of 1.536% using UEE was achieved under the following optimal conditions: electrostatic field of 7kV, ultrasonic power of 500W, ethanol concentration of 70% and extraction time of 20min. The optimal solid-liquid ratio (1:25g/mL) and extraction temperature (55°C) were determined by single factor experiments. Compared to other extraction methods, UEE not only increases the extraction yield of TF but also exhibits an excellent antioxidant activity in assays of the scavenging capacity for DPPH, hydroxyl and superoxide anion radicals. The availability of the UEE method can be supported by the ultrasonic cavitation effect, which plays the most important role in the UEE method. The electrostatic field can be regarded as a random disturbance for sonication, which can strengthen the cavitation effect and increase the cavitation yield. Moreover, the amount of iodine release in potassium iodide solution well validated the synergetic effect between the ultrasound and electrostatic field.

Intervene effect on Streptococcus suis biofilm formation of emodin extracted from Rheum officinale Baill

“…However, the extracted emodin may be degraded and its structure may be destroyed when the time is too long in the ultrasonic surrounding [49]. On effect of ethanol concentration, the effect tendency was consistent with the reports on the extraction of orientin and avonoids [50,51]. On one hand, dielectric constant of extraction reduces with the amount of ethanol increasing, and then solvent solubility and diffusion of target components are improved, which in turn result in the high yield of emodin [52].…”

Optimization of Cellulase-Assisted Extraction of Total Flavonoids from Corn Bract and Evaluation of Antioxidant and Antibacterial Aactivities

“…Flavonoids are a kind of compounds widely distributed in plants as secondary metabolites (Zakaryan et al, 2017). An increasing number of studies have reported the extraction methods of flavonoids from different plant sources (Liu et al, 2016;Jing et al, 2016;Wang et al, 2017;Yang et al, 2017;Yu et al, 2017). Enzymeassisted extraction is established as an emerging technique to accelerate the release of bioactive components from plant materials, due to its advantages of high extraction yield, environmental compatibility, low energy requirement and simplified manipulation (Chen et al, 2011).…”

A rapid and efficient extraction method based on industrial MCM‐41‐miniaturized matrix solid‐phase dispersion extraction with response surface methodology for simultaneous quantification of six flavonoids in Pollen typhae by ultra‐high‐performance liquid chromatography

“…To further investigate the practicability of the proposed method, a comparison with other methods existed for flavonoid extraction was performed in terms of extraction method, solvent, extraction time, analysis method and time (Table ). It was found that these reported methods required frequently a large amount of sample (more than 0.5 g) and extraction solvent (more 10 mL) while the current industrial MCM‐41‐MMSPD extraction method only required the milligram amounts of sample (25 mg) and reagent (1.25 mL) . In addition, most of these methods mainly focused on a few flavonoids or total flavonoids, which were insufficient for evaluation quality of TCMs with multi‐compounds.…”

“…UHPLC is selected in our study to realize the rapid analysis and determination of multi‐components in Pollen typhae based on its superiority of small injection volume, low mobile phase consumption, and short analysis time. Based on the present analysis methods, there are series of extract methods for herbal products such as heating reflux extraction , ultrasound method, microwave extraction , and solid phase extraction . These methods usually require many samples and organic reagents, long extract period, high temperature or energy, and complex equipment.…”