Ultraspecific electrochemical DNA biosensor by coupling spontaneous cascade DNA branch migration and dual-signaling sensing strategy

Wang, Ting; Zhou, Lili; Bai, Shulian; Zhang, Zhang; Li, Junlong; Jing, Xiaoying

doi:10.1016/j.bios.2015.11.071

Cited by 28 publications

(11 citation statements)

References 30 publications

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“…The most common techniques are DNA sensors based on optical,, electrochemical,,,, and gravimetric, transductors or combinations thereof . Many DNA sensors are based on labelled probes, targets, or reporter strands . In earlier papers, we reported osmium‐tetroxide‐labeled nucleic acids on unheated and heated electrodes ,.…”

Section: Figurementioning

confidence: 99%

Redox‐Induced Switching of the Viscoelasticity of DNA Layers Observed by using Electrochemical Quartz Crystal Microbalance on the Millisecond Timescale

2017

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The interactions of single‐stranded DNA (ssDNA) immobilized on gold electrodes with hexammine ruthenium(III) (RuHex) and hexammine cobalt(III) (CoHex) were investigated with chronocoulometric and electrochemical quartz crystal microbalance experiments. The modified surfaces were created by immobilizing ssDNA with tris(dithioserinol) linkers to form a compact, self‐assembled monolayer, followed by passivation with 6‐mercapto‐1‐hexanol (MCH). Upon addition of the redox indicator during a potential jump for RuHex and CoHex, we observed a dramatic increase in the frequency response in comparison to the signal observed with only low‐ionic‐strength hybridization buffer, 10 mM tris(hydroxymethyl)‐aminomethane (Tris)/H2SO4 (pH 7.4). RuHex and CoHex responded quite differently, even on a millisecond time scale. As the expected mass change is less than a factor of 35 to the observed frequency response, we concluded that the applied potential jumps lead to switching of the DNA layer viscoelasticity.

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Section: Figurementioning

confidence: 99%

Redox‐Induced Switching of the Viscoelasticity of DNA Layers Observed by using Electrochemical Quartz Crystal Microbalance on the Millisecond Timescale

2017

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“…Multiple methodologies have been developed to obtain the selectivity required for single point mutation discrimination, and their incorporation with ratiometric electrochemical technique has led to reliable, accurate biosensors. Xie et al demonstrated that cascade DNA branch migration could be employed with dual-redox labels for selective and robust DNA detection (Figure 10) [33]. A two-component approach was utilised with a 3 -MB label at the end of one signal probe, which was complementary to a capture probe immobilised onto a AuE, and a second signal probe labelled 3 -Fc, which was complementary to that of a protection strand.…”

Section: Selectivity Strategiesmentioning

confidence: 99%

Ratiometric Electrochemistry: Improving the Robustness, Reproducibility and Reliability of Biosensors

Spring

Goggins²,

Frost

2021

Molecules

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Electrochemical biosensors are an increasingly attractive option for the development of a novel analyte detection method, especially when integration within a point-of-use device is the overall objective. In this context, accuracy and sensitivity are not compromised when working with opaque samples as the electrical readout signal can be directly read by a device without the need for any signal transduction. However, electrochemical detection can be susceptible to substantial signal drift and increased signal error. This is most apparent when analysing complex mixtures and when using small, single-use, screen-printed electrodes. Over recent years, analytical scientists have taken inspiration from self-referencing ratiometric fluorescence methods to counteract these problems and have begun to develop ratiometric electrochemical protocols to improve sensor accuracy and reliability. This review will provide coverage of key developments in ratiometric electrochemical (bio)sensors, highlighting innovative assay design, and the experiments performed that challenge assay robustness and reliability.

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“…Additionally, combined transductors are well known. [22] Many electrochemical DNA sensors use covalently labeled probes, [23] targets, [24,25] and reporter or signaling strands. [26] DNA strands labeled with osmium tetroxide bipyridine have been used for hybridization detection on unheated and heated gold electrodes.…”

Section: Voltammetric H/d Isotope Effects On Redox-active Small Molecmentioning

confidence: 99%

Voltammetric H/D Isotope Effects on Redox‐Active Small Molecules Conjugated with DNA Self‐Assembled Monolayers

Galagedera

Flechsig

2019

ChemElectroChem

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This paper describes the largest H/D kinetic isotope effect (KIE) at room temperature (25 °C) reported to date (kH/kD=2400). In voltammetric measurements with DNA monolayers on gold electrodes, a maximum shift of −400 mV was recorded for the reduction peak potential of 50 μM hexammine cobalt(III) (CoHex) in 10 mM Tris buffer upon replacing water with deuterium oxide in the electrolyte. In subsequent comparative investigations, a much smaller shift (ca. −80 mV) was recorded with daunomycin, whereas no potential shift was recorded with hexammine ruthenium(III) (RuHex). The interactions of RuHex, CoHex, and daunomycin with a mixed self‐assembled monolayer of single‐stranded DNA and 6‐mercapto‐1‐hexanol (ssDNA/MCH SAM) immobilized on gold electrodes were studied by using cyclic and differential‐pulse voltammetry (CV and DPV, respectively). The hydrogen‐bond network within the ssDNA layer seems to amplify the voltammetric H/D isotope effect with CoHex. Voltammetric studies of H/D isotope effects can provide a platform to investigate amplified isotope effects probably not only on DNA layers, but also on proteins and small organic molecules and may be useful for studies of cell proliferation as well as drug testing.

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Ultraspecific electrochemical DNA biosensor by coupling spontaneous cascade DNA branch migration and dual-signaling sensing strategy

Cited by 28 publications

References 30 publications

Redox‐Induced Switching of the Viscoelasticity of DNA Layers Observed by using Electrochemical Quartz Crystal Microbalance on the Millisecond Timescale

Redox‐Induced Switching of the Viscoelasticity of DNA Layers Observed by using Electrochemical Quartz Crystal Microbalance on the Millisecond Timescale

Ratiometric Electrochemistry: Improving the Robustness, Reproducibility and Reliability of Biosensors

Voltammetric H/D Isotope Effects on Redox‐Active Small Molecules Conjugated with DNA Self‐Assembled Monolayers

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