Ultrastable Liquid–Liquid Interface as Viable Route for Controlled Deposition of Biodegradable Polymer Nanocapsules

Abstract

Liquid-liquid interfaces are highly dynamic and characterized by an elevated interfacial tension as compared to solid-liquid interfaces. Therefore, they are gaining an increasing interest as viable templates for ordered assembly of molecules and nanoparticles. However, liquid-liquid interfaces are more difficult to handle compared to solid-liquid interfaces; their intrinsic instability may affect the assembly process, especially in the case of multiple deposition. Indeed, some attempts have been made in the deposition of polymer multilayers at liquid-liquid interfaces, but with limited control over size and stability. This study reports on the preparation of an ultrastable liquid-liquid interface based on an O/W secondary miniemulsion and its possible use as a template for the self-assembly of polymeric multilayer nanocapsules. Such polymer nanocapsules are made of entirely biodegradable materials, with highly controlled size-well under 200 nm-and multi-compartment and multifunctional features enriching their field of application in drug delivery, as well as in other bionanotechnology fields.

<p>Nano-Encapsulation of Coenzyme Q10 in Secondary and Tertiary Nano-Emulsions for Enhanced Cardioprotection and Hepatoprotection in Human Cardiomyocytes and Hepatocytes During Exposure to Anthracyclines and Trastuzumab</p>

“…O/W NEs were produced as previously described. 27 Because of the importance of scaling down the size in terms of bioavailability once in vivo, 28 it was decided to use the smallest size, among different possible ones that can be tuned with the amount of surfactants. A complete dimensional and morphological study of the simple O/W NEs was performed by DLS and Cryo-TEM analysis.…”

“…Primary NEs were prepared adapting a previously developed protocol. 27 , 28 Briefly, first, the oil phase was prepared by adding the surfactant to the soybean oil. A 5.8 g of Lipoid E 80 was dissolved in 24 mL of soybean oil at 60°C and mixed using the immersion sonicator (Ultrasonic Processor VCX500 Sonic and Materials).…”

“…The second layer of hyaluronic acid (HA) was prepared with the aid of two syringe pumps (HARVARD APPARATUS 11 PLUS) and an ultrasonic bath (FALC INSTRUMENTS) according to a previously reported method. 28 Starting from the secondary NEs 10 wt % oil - 0.1 wt % CT, a polymer layer was deposited by mixing 1:1 (v:v) of a 0.24 wt % aqueous solution of HA, with the secondary NEs suspension. The two liquid phases were injected at the same flow rate (0.4 mL·min −1 ) through two micrometric capillaries interfaced at their extremities.…”

Cardioprotective Effects of Nanoemulsions Loaded with Anti-Inflammatory Nutraceuticals against Doxorubicin-Induced Cardiotoxicity

“…Nanoemulsions were prepared following a previously developed protocol [ 22 ] which can also be adapted to the encapsulation of contrasting agents [ 23 ]. Briefly, first the oil phase was prepared by adding the surfactant to the soybean oil and mixed at 60 °C under gentle stirring.…”

Chitosan-coated liposomes loaded with butyric acid demonstrate anticancer and anti-inflammatory activity in human hepatoma HepG2 cells

“…The recent use of nanotechnology in medicine has brought important advances in the delivery of drugs in inflamed and cancer tissues. In light of this, liposomes are lipidic nanocarriers able to protect a drug from the external environment, enzymatic attack and immune recognition with consequently increased bioavailability, controlled drug delivery, biodistribution in targets such as cancer tissues and reduced toxicity (30–32). Liposomes and other nanocarriers are directly involved in altering the biodistribution of certain anticancer agents that cannot be efficiently delivered, in their free formulation, in cancer tissues or cannot be appropriately adsorbed by the gut (33).…”