Ultrastrong and flexible hybrid hydrogels based on solution self-assembly of chitin nanofibers in gelatin methacryloyl (GelMA)

Hassanzadeh, Pegah; Kazemzadeh-Narbat, Mehdi; Rosenzweig, Roy; Zhang, Xiaolin; Khademhosseini, Ali; Annabi, Nasim; Rolandi, Marco

doi:10.1039/c6tb00021e

Cited by 70 publications

(47 citation statements)

References 31 publications

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“…The nanofiber‐gelatin films did not induce inflammation and strongly promoted fibroblast proliferation, indicating high biocompatibility and bioactivity. In another work, chitin nanofibers‐GelMA nanocomposites were prepared via a self‐assembly approach, yielding ultra‐strong and flexible hydrogels . Compared to GelMA, the elastic modulus of these hydrogels was increased by ~1,000 folds, and the composite gel was 100 and 200% more extensible than chitin or GelMA, respectively.…”

Section: Gelatin–polysaccharides Composites In Cell Culture and Tissumentioning

confidence: 99%

“…Compared to GelMA, the elastic modulus of these hydrogels was increased by ~1,000 folds, and the composite gel was 100 and 200% more extensible than chitin or GelMA, respectively. These hydrogels were used as scaffolds for human umbilical vein endothelial cells (HUVECs) cocultured with human mesenchymal stem cells (HMSCs), which provided enhanced cellular differentiation and vascular network formation due to the increased flexibility and elastic modulus …”

Section: Gelatin–polysaccharides Composites In Cell Culture and Tissumentioning

confidence: 99%

“…These hydrogels were used as scaffolds for human umbilical vein endothelial cells (HUVECs) cocultured with human mesenchymal stem cells (HMSCs), which provided enhanced cellular differentiation and vascular network formation due to the increased flexibility and elastic modulus. 141 Embedding nanohydroxyapatite in β-chitin-gelatin composites has enabled 3D cell culture for bone repair and regeneration. 142 The cytocompatibility study of these scaffolds in mouse preosteoblast cells suggested that the cell behavior inside the microenvironment was regulated by the ions released from the hydroxyapatite particles.…”

Section: Gelatin-chitinmentioning

confidence: 99%

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Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

Afewerki

Sheikhi

Kannan

et al. 2018

Bioengineering & Transla Med

309

210

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Gelatin is a promising material as scaffold with therapeutic and regenerative characteristics due to its chemical similarities to the extracellular matrix (ECM) in the native tissues, biocompatibility, biodegradability, low antigenicity, cost‐effectiveness, abundance, and accessible functional groups that allow facile chemical modifications with other biomaterials or biomolecules. Despite the advantages of gelatin, poor mechanical properties, sensitivity to enzymatic degradation, high viscosity, and reduced solubility in concentrated aqueous media have limited its applications and encouraged the development of gelatin‐based composite hydrogels. The drawbacks of gelatin may be surmounted by synergistically combining it with a wide range of polysaccharides. The addition of polysaccharides to gelatin is advantageous in mimicking the ECM, which largely contains proteoglycans or glycoproteins. Moreover, gelatin–polysaccharide biomaterials benefit from mechanical resilience, high stability, low thermal expansion, improved hydrophilicity, biocompatibility, antimicrobial and anti‐inflammatory properties, and wound healing potential. Here, we discuss how combining gelatin and polysaccharides provides a promising approach for developing superior therapeutic biomaterials. We review gelatin–polysaccharides scaffolds and their applications in cell culture and tissue engineering, providing an outlook for the future of this family of biomaterials as advanced natural therapeutics.

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Section: Gelatin–polysaccharides Composites In Cell Culture and Tissumentioning

confidence: 99%

Section: Gelatin–polysaccharides Composites In Cell Culture and Tissumentioning

confidence: 99%

Section: Gelatin-chitinmentioning

confidence: 99%

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Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

Afewerki

Sheikhi

Kannan

et al. 2018

Bioengineering & Transla Med

309

210

View full text Add to dashboard Cite

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“…For example, cartilage, containing 75 wt% of water, exhibits an compressive strength of 36 MPa, 2 which allows the cartilage to endure a daily compression of several MPa. 3 Recently, some successful strategies were employed to prepare hydrogels with attractive properties, such as double network hydrogels, [4][5][6][7][8][9] nanocomposite hydrogels, [10][11][12][13][14] and physical interaction enhanced hydrogels. [15][16][17][18] Based on these design principles, rapid progress has been made in the preparation of robust and flexible hydrogels with controllable microstructures and excellent mechanical properties.…”

Section: Introductionmentioning

confidence: 99%

Synthetic self-assembled homogeneous network hydrogels with high mechanical and recoverable properties for tissue replacement

Liu

et al. 2016

J. Mater. Chem. B

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Development of hydrogels with highly mechanical and recoverable properties under physiological conditions is of great importance for broadening and improving their potential applications in load-bearing artificial soft tissues. Inspired by the self-assembly of chemistry entities, the homogeneous network hydrogels, which contain over 90 wt% of water, were synthesized from covalent cross-links of poly(vinyl alcohol) (PVA) and poly(vinyl pyrrolidone) (PVP) triggered by microwave-assisted treatment. Structurally homogeneous network results in a evenly distributed stress that endure high strains with minimal energy dissipation, which enable the hydrogels to withstand up to 1.16 MPa of tensile stress, over seven-fold of stretch length with negligible hysteresis, and sustain cyclic compression following high amplitude deformation. It is of importance for tissue replacement that the hydrogels retain these excellent properties under physiological conditions.

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“…Another principle used to enhance the mechanical properties of hydrogels is the use of nanofibers. These can be used to enforce a hydrogel by forming an internal random network [22] or, by aligning them, increasing the tensile strength [23] or tune the matrix compressive modulus [24].…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Current developments in 3D bioprinting for tissue engineering

Cornelissen

Faulkner-Jones

Shu

2017

Current Opinion in Biomedical Engineering

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This version is available at https://strathprints.strath.ac.uk/61660/ Strathprints is designed to allow users to access the research output of the University of Strathclyde. Unless otherwise explicitly stated on the manuscript, Copyright © and Moral Rights for the papers on this site are retained by the individual authors and/or other copyright owners. Please check the manuscript for details of any other licences that may have been applied. You may not engage in further distribution of the material for any profitmaking activities or any commercial gain. You may freely distribute both the url (https://strathprints.strath.ac.uk/) and the content of this paper for research or private study, educational, or not-for-profit purposes without prior permission or charge.Any correspondence concerning this service should be sent to the This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPTCurrent developments in 3D bioprinting for tissue engineering AbstractThe field of 3-dimensional (3D) bioprinting have enjoyed rapid development in the past few years for the applications in tissue engineering and regenerative medicine. In this review, we summarize the most updated developments in 3D bioprinting for the applications in the tissue engineering with a focus on the printable biomaterials used as bioinks. These developments include 1) novel printing regimes have been enabled by the use of fugitive inks for the creation of intricate structures e.g. vascularized tissue constructs; 2) mechanical strength of printed constructs can be enhanced by co-printing soft and hard biomaterials; 3) bioprinted in-vitro models for drug testing applications are closer to reality. We conclude that the research and application of new bioinks will remain the key highlights of the future developments in 3D bioprinting for tissue engineering.

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Ultrastrong and flexible hybrid hydrogels based on solution self-assembly of chitin nanofibers in gelatin methacryloyl (GelMA)

Abstract: We demonstrate ultrastrong and flexible hydrogels by self-assembling chitin nanofiber in the presence of gelatin methacryloyl. We tune the mechanical properties of the hydrogel with chitin nanofiber content and show proof-of-concept applications in engineering vascular tissue.

Cited by 70 publications

References 31 publications

Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

Synthetic self-assembled homogeneous network hydrogels with high mechanical and recoverable properties for tissue replacement

Current developments in 3D bioprinting for tissue engineering

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