Ultrastructural alteration in Gill and Hepatopancrease of freshwater prawn Macrobrachium rosenbergii exposed to 60Co gamma radiation

Stalin, A.; Palani, Suganthi; Mathivani, S; Gokula, V.

doi:10.1007/s11356-020-11394-8

Cited by 6 publications

(1 citation statement)

References 19 publications

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“…At 48 h, gills from 6 shrimps per group were sampled to test scRNA and gills from 3 shrimps per group were sampled to test gene expression. at 4 °C for 24 h and were post-fixed by 1% OsO 4 for 2 h. After dehydration in ascending graded ethanol (30%-50%-70%-80%-95%-100%-100% ethanol concentrations orderly, 20 min at a time) and clearness by 100% acetone twice (15 min at a time), tissues were molded with epoxy resin, kept in an incubator at 60 °C for 48 h, cooled down, sectioned (60 nm-80 nm) through ultramicrotome (Leica ultracut UCT) with a glass knife, and stained by 2% uranium acetate saturated alcohol solution avoid light staining for 8 min and 2.6% Lead citrate avoid CO 2 (Arumugam et al, 2021).…”

Section: Animals and High Ph Stress Experimentsmentioning

confidence: 99%

Highly sensitive and specific responses of shrimp gill cells to high pH stress based on single cell RNA-seq analysis

Wang

et al. 2022

Front. Cell Dev. Biol.

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High pH is one of the main stressors affecting the shrimp survival, growth, and physiology in aquaculture ponds, but the cellular and molecular mechanism responsible for high pH stress has not been elucidated in shrimp. In this study, the shrimp acid-base disturbance and gill cell alterations were significantly observed and then single cell RNA-sequencing (scRNA-seq) was performed to study the sensitive and specific responses of gill cells to high pH stress. Three main gill cell types, including pillar cells, hemocytes and septal cells were identified. By comparative scRNA-seq analysis between control and pH group, the pillar cell was regarded as the target cell type in response to high pH stress with the down-regulation of ammonia excretion and H+ transport related genes and up-regulation of immune related genes. Notedly, high pH resulted in the emergence of a new immune cell subcluster in pillar cells, with immune activation and stress defense states. Pseudotime analysis also showed that the pillar cells could transform into the functionally inhibited ion cell subclusters and functionally activated immune cell subclusters after high pH stress. Further, the regulatory network of pillar cell population was predicted by WGCNA and two transcription factors were identified. In conclusion, these results provide key insights into the shrimp gill cell-type-specific mechanisms underlying high pH stress response at a single-cell resolution.

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Section: Animals and High Ph Stress Experimentsmentioning

confidence: 99%

Highly sensitive and specific responses of shrimp gill cells to high pH stress based on single cell RNA-seq analysis

Wang

et al. 2022

Front. Cell Dev. Biol.

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Assessing Metal Toxicity on Crustaceans in Aquatic Ecosystems: A Comprehensive Review

Banaee,

Zeidi,

Mikušková

et al. 2024

Biol Trace Elem Res

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Effect of high alkalinity on shrimp gills: Histopathological alternations and cell specific responses

Wang

et al. 2023

Ecotoxicology and Environmental Safety

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Ultrastructural alteration in Gill and Hepatopancrease of freshwater prawn Macrobrachium rosenbergii exposed to 60Co gamma radiation

Cited by 6 publications

References 19 publications

Highly sensitive and specific responses of shrimp gill cells to high pH stress based on single cell RNA-seq analysis

Highly sensitive and specific responses of shrimp gill cells to high pH stress based on single cell RNA-seq analysis

Assessing Metal Toxicity on Crustaceans in Aquatic Ecosystems: A Comprehensive Review

Effect of high alkalinity on shrimp gills: Histopathological alternations and cell specific responses

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