Ultrastructural analysis of the glutamatergic system in the outer plexiform layer of zebrafish retina

Klooster, Jan; Yazulla, Stephen; Kamermans, Maarten

doi:10.1016/j.jchemneu.2009.02.004

Cited by 29 publications

(33 citation statements)

References 70 publications

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“…NTPDase1-IR is also present in horseshoe-shaped structures characteristic for localization in HC dendrites. (E) Double labeling of NTPDase1 antibody with an antibody against GluR2 (green), a marker for HC dendrites [63],[64], indicated that NTPDase-1 (red) was specifically expressed on HC dendrites. (F) ADA-IR (green) was present in horseshoe-shaped structures.…”

Section: Resultsmentioning

confidence: 99%

Extracellular ATP Hydrolysis Inhibits Synaptic Transmission by Increasing pH Buffering in the Synaptic Cleft

et al. 2014

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Section: Resultsmentioning

confidence: 99%

Extracellular ATP Hydrolysis Inhibits Synaptic Transmission by Increasing pH Buffering in the Synaptic Cleft

et al. 2014

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“…Electron microscopy analysis. For immuno-electron microscopy (immuno-EM) analysis, eyes were processed according to the procedures described previously (Klooster et al, 2009). Briefly, eyes were fixed for 1 h at room temperature in 0.1 M phosphate-buffered (pH 6.5) 4% formaldehyde.…”

Section: Methodsmentioning

confidence: 99%

PALS1 Is Essential for Retinal Pigment Epithelium Structure and Neural Retina Stratification

et al. 2011

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The membrane-associated palmitoylated protein 5 (MPP5 or PALS1) is thought to organize intracellular PALS1-CRB-MUPP1 protein scaffolds in the retina that are involved in maintenance of photoreceptor-Müller glia cell adhesion. In humans, the Crumbs homolog 1 (CRB1) gene is mutated in progressive types of autosomal recessive retinitis pigmentosa and Leber congenital amaurosis. However, there is no clear genotype-phenotype correlation for CRB1 mutations, which suggests that other components of the CRB complex may influence the severity of retinal disease. Therefore, to understand the physiological role of the Crumbs complex proteins, especially PALS1, we generated and analyzed conditional knockdown mice for Pals1. Small irregularly shaped spots were detected throughout the PALS1 deficient retina by confocal scanning laser ophthalmoscopy and spectral domain optical coherence tomography. The electroretinography a-and b-wave was severely attenuated in the aged mutant retinas, suggesting progressive degeneration of photoreceptors. The histological analysis showed abnormal retinal pigment epithelium structure, ectopic photoreceptor nuclei in the subretinal space, an irregular outer limiting membrane, half rosettes of photoreceptors in the outer plexiform layer, and a thinner photoreceptor synaptic layer suggesting improper photoreceptor cell layering during retinal development. The PALS1 deficient retinas showed reduced levels of Crumbs complex proteins adjacent to adherens junctions, upregulation of glial fibrillary acidic protein indicative of gliosis, and persisting programmed cell death after retinal maturation. The phenotype suggests important functions of PALS1 in the retinal pigment epithelium in addition to the neural retina.

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“…AMPA receptors, however, appear to be the dominant receptors in salamander retina (Cadetti et al, 2005; Maple et al, 1999). In zebrafish, AMPA receptor subunit GluA4 is observed on OFF-bipolar dendrites innervating both rod and cone terminals (Klooster et al, 2009). At cone pedicles of the macaque retina, basal contacts of OFF-cone bipolar cells have been shown to contain GluA1-subunit containing AMPA receptors (Haverkamp et al, 2000), GluK1 and GluK2/3-subunit containing Kainate receptors and GluA2/3 and 4 subunits of AMPA receptors (Calkins, 2005; Haverkamp et al, 2000, 2001a).…”

Section: Synapse Structure and Connectivity Of Retinal Neuronsmentioning

confidence: 99%

“…Horizontal cell processes contain iGluRs with a predominance of AMPA (GluA2/3 and 4 subunits) receptors in macaque (Calkins, 2005; Haverkamp et al, 2000, 2001a, b). In zebrafish, immunoreactivity for the AMPA receptor GluA2 is observed in horizontal processes invaginating both rod and cone photoreceptor terminals (Klooster et al, 2009). Taken together, horizontal cell processes appear more dependent upon AMPA receptors for signal processing at the OPL, whereas OFF-bipolar dendrites (depending on species and subtype) rely with different weights on Kainate and AMPA receptors; Kainate receptors predominantly mediating transmission onto OFF-bipolar cells in mouse and macaque retina.…”

Section: Synapse Structure and Connectivity Of Retinal Neuronsmentioning

confidence: 99%

Functional architecture of the retina: Development and disease

Hoon

Okawa

Santina

et al. 2014

Progress in Retinal and Eye Research

469

385

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Structure and function are highly correlated in the vertebrate retina, a sensory tissue that is organized into cell layers with microcircuits working in parallel and together to encode visual information. All vertebrate retinas share a fundamental plan, comprising five major neuronal cell classes with cell body distributions and connectivity arranged in stereotypic patterns. Conserved features in retinal design have enabled detailed analysis and comparisons of structure, connectivity and function across species. Each species, however, can adopt structural and/or functional retinal specializations, implementing variations to the basic design in order to satisfy unique requirements in visual function. Recent advances in molecular tools, imaging and electrophysiological approaches have greatly facilitated identification of the cellular and molecular mechanisms that establish the fundamental organization of the retina and the specializations of its microcircuits during development. Here, we review advances in our understanding of how these mechanisms act to shape structure and function at the single cell level, to coordinate the assembly of cell populations, and to define their specific circuitry. We also highlight how structure is rearranged and function is disrupted in disease, and discuss current approaches to re-establish the intricate functional architecture of the retina.

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Ultrastructural analysis of the glutamatergic system in the outer plexiform layer of zebrafish retina

Cited by 29 publications

References 70 publications

Extracellular ATP Hydrolysis Inhibits Synaptic Transmission by Increasing pH Buffering in the Synaptic Cleft

Extracellular ATP Hydrolysis Inhibits Synaptic Transmission by Increasing pH Buffering in the Synaptic Cleft

PALS1 Is Essential for Retinal Pigment Epithelium Structure and Neural Retina Stratification

Functional architecture of the retina: Development and disease

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