Ultrastructural and biochemical alterations during browning of pigeon orchid (Dendrobium crumenatum Swartz) callus

Kaewubon, Paveena; Hutadilok‐Towatana, Nongporn; Silva, Jaime A. Teixeira da; Meesawat, Upatham

doi:10.1007/s11240-014-0678-y

Cited by 25 publications

(17 citation statements)

References 83 publications

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“…The biochemical and ultrastructural alterations associated with callus browning were examined by using TEM and SEM in calli from Dendrobium crumenatum Swarts (29). Calli were fixed in 2.5% GA with 0.1 M phosphate buffer and post-fixed in 1% caffeine and 1% OsO 4 solutions.…”

Section: Discussionmentioning

confidence: 99%

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Calli Ultrastructure of Globularia trichosantha ssp. trichosantha

Atar¹

2017

Period. Biol.

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Section: Discussionmentioning

confidence: 99%

“…Various organelles in 3-month-old callus cells were completely disorganized. Brown cells had minimal cytoplasm, numerous degraded thylakoid-containing plastids and shrunken plasmalemma resulting in detachment of the plasmalemma from the cell wall (29).…”

Section: Discussionmentioning

confidence: 99%

Calli Ultrastructure of Globularia trichosantha ssp. trichosantha

Atar¹

2017

Period. Biol.

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“…Browning results from the accumulation of phenolic compounds that cause the loss of growth capacity and tissue death during Dendrobium tissue culture (Kaewubon and Meesawat 2009;Rittirat et al 2012;Kaewubon et al 2015). Browning was also observed in this study, with the percentage of PLB browning ranging between 12 and 54% (Tables 1, 2, and 3), and is presumably caused by surface damage of PLBs during incubation in liquid shake culture as reported in previous studies of Dendrobium 'Gradita 31' (12.3% brown PLBs; Winarto and Rachmawati 2013) and Dendrobium 'Zahra FR 62' (35.1% brown PLBs; .…”

Section: Discussionmentioning

confidence: 99%

“…Periodic subcultures of plant tissue cultures have been applied for several purposes: increasing or multiplying the number of explants, maintaining the regeneration capacity or juvenility of explants, or increasing the health of cultures ; inducing in vitro flowering (Kanchanapoom et al 2009(Kanchanapoom et al , 2010; or reducing (Jaskani et al 2008;Kaewubon et al 2015). A monthly subculture of Dendrobium 'Gradita 31' PLBs on three media resulted in a persubculture increase in the number of PLBs until the third subculture, following a trend also observed for Dendrobium 'Gradita 31' and Dendrobium 'Zahra FR 62' .…”

Section: Discussionmentioning

confidence: 99%

Use of coconut water and fertilizer for in vitro proliferation and plantlet production of Dendrobium ‘Gradita 31’

Winarto¹,

Silva

2015

In Vitro Cell.Dev.Biol.-Plant

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To improve the micropropagation of Dendrobium 'Gradita 31', coconut water (CW) and fertilizer media were used to enhance growth, proliferation, and germination of protocorm-like bodies (PLBs). PLBs formed when shoots approximately 0.4 cm long were cultured on semi-solid halfstrength Murashige and Skoog (MS) basal medium containing 1 mg L − 1 thidiazuron (TDZ) and 0.5 mg L − 1 N 6 -benzyladenine (BA). After four rounds of 15-d subcultures, PLBs were initially proliferated in liquid half-strength MS medium supplemented with 0.3 mg L − 1 TDZ and 0.1 mg L −1 α-naphthalene acetic acid (NAA) and subcultured monthly into fresh medium. When 15% (v/v) CW was added to half-strength MS medium, optimal growth and proliferation of PLBs resulted with the following principal findings: a maximum of 2.86 g total fresh weight (FW) of PLBs formed from 1 g of PLBs, an average of 0.46 g FW of PLBs added per subculture period, a total of 157.1 PLBs formed, an average of 25.3 PLBs added per subculture period, and a low percentage of PLB browning (20.7%). Half-strength MS medium containing 15% (v/v) CW with Rosasol ® medium (liquid fertilizer; 1.5 g L −1 18 N:18P:18 K+1.5 g L −1 25 N:10P:10 K+ TE) resulted in a similar organogenic outcome with 25.5% PLB browning. PLBs germinated easily and rooted on Rosasol-supplemented plant growth regulator-free medium.In total, 180 Dendrobium 'Gradita 31' plantlets from all treatments were successfully acclimatized with 100% survival on a Cycas rumphii bulk substrate and grew well after repotting in a mixture of wood charcoal and C. rumphii bulk (1:1, v/v).

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“…When cultured on a Murashige and Skoog (1962;MS) basal medium without plant growth regulators (PGRs), D. draconis tTCLs remain green for up to 1 -2 weeks and then gradually become brown and die (Rangsayatorn 2009). Browning or blackening explants and calluses occurs as a result of phenolic secretions from the wounded cells, during sectioning tissue from shoot tips, leaves, or PLBs (Kaewubon et al 2015). The incidence of browning in the medium and explants on TCL cultures were frequently reported in several orchids such as D. fimbriatum, D. chrysotoxum, D. candidum, D. draconis, D. nobile, Cymbidium spp., and Phalaenopsis cornu-cervi (Roy and Banerjee 2003, Malabadi et al 2005, Teixeira da Silva et al 2006, Roy et al 2007, Zhao et al 2007, Rangsayatorn 2009, Vyas et al 2010, Rittirat et al 2012.…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis from stem thin cell layers of Dendrobium aqueum

Parthibhan

Rao

Silva³

et al. 2018

Biologia plant.

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An efficient in vitro regeneration protocol through somatic embryogenesis was established from stem transverse thin cell layers (tTCLs) of Dendrobium aqueum Lindley, an imperiled orchid. This study outlines the induction and successive maturation stages of D. aqueum somatic embryos (SEs). The tTCLs (~ 0.5 mm thick) cultured on halfstrength Murashige and Skoog (MS) medium containing cytokinins and auxins, either individually or in combination, produced embryogenic callus (EC). Treatment with 0.5 mg dm -3 zeatin induced EC in 41.42 % of tTCLs. As many as 42.66 globular SEs per tTCL were formed in the presence of 1.5 mg dm -3 N 6 -(2-isopentyl) adenine (2iP) but only on 10.33 % of explants. The combined treatment of 2iP (1.5 mg dm -3 ) and 0.5 mg dm -3 6-benzyladenine resulted in 34 globular SEs on 14.7 % of tTCLs whereas the combination of 2iP and 1.0 mg dm -3 indole-3-butyric acid (IBA) induced 7.4 globular SEs on 52.33 % of tTCLs. Supplementation of activated charcoal, amino acids, and antioxidants alleviated browning at all the concentrations tested, but the EC response declined. The addition of 0.5 mg dm -3 polyvinylpyrrolidone to 1.5 mg dm -3 2iP and 1.0 mg dm -3 IBA produced 24 SEs on 19.89 % of tTCLs suggesting that the EC and SEs can be effectively induced by individual cytokinins whereas the synergistic treatments with other compounds can only enhance the induction of EC. Histological observations of EC showed the formation of globular SEs from sub-epidermal regions. Successive developmental stages of globular SEs and the intermediate stage of protocorm like bodies until the formation of plantlets were observed. The plantlets obtained through SEs showed no morphological variations, and inter simple sequence repeat profiles also confirmed the genetic fidelity of in vitroderived progeny with high monomorphism (97.78 %). In conclusion, the use of stem tTCLs is an effective method to produce SEs through indirect somatic embryogenesis in D. aqueum.

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Ultrastructural and biochemical alterations during browning of pigeon orchid (Dendrobium crumenatum Swartz) callus

Cited by 25 publications

References 83 publications

Calli Ultrastructure of Globularia trichosantha ssp. trichosantha

Calli Ultrastructure of Globularia trichosantha ssp. trichosantha

Use of coconut water and fertilizer for in vitro proliferation and plantlet production of Dendrobium ‘Gradita 31’

Somatic embryogenesis from stem thin cell layers of Dendrobium aqueum

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