Ultrastructural and chemical characterization of egg surface of honeybee worker and queen-laid eggs

Katzav-Gozansky, Tamar; Soroker, Victoria; Kamer, J. C. van de; Schulz, Claudia; Francke, Wittko; Abraham, Hefetz

doi:10.1007/s00049-003-0238-0

Cited by 33 publications

(18 citation statements)

References 28 publications

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“…In the honey bee A. mellifera, worker policing of eggs occurs as well (52); this has led to a number of studies of the compounds found on queenand worker-laid eggs and the compounds present in the queen's Dufour's gland (53) and in egg-laying workers (54)(55)(56), which appear to mimic queen specific profiles. Although there is experimental evidence that the egg identification cue originates from Dufour's gland secretions (57), behavioral experiments have failed so far to show which of the many compounds are active (58)(59)(60), and it appears that in this case hydrocarbons are not involved (58).…”

Section: Discussionmentioning

confidence: 99%

Surface hydrocarbons of queen eggs regulate worker reproduction in a social insect

Endler

Liebig

Schmitt

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

258

219

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A hitherto largely unresolved problem in behavioral biology is how workers are prevented from reproducing in large insect societies with high relatedness. Signals of the queen are assumed to inform the nestmates about her presence in the colony, which leads to indirect fitness benefits for workers. In the ant Camponotus floridanus, we found such a signal located on queen-laid eggs. In groups of workers that were regularly provided with queen-laid eggs, larvae, and cocoons, with larvae and cocoons alone, or with no brood, only in the groups with queen-laid eggs did workers not lay eggs. Thus, the eggs seem to inform the nestmates about the queen's presence, which induces workers to refrain from reproducing. The signal on queen-laid eggs is presumably the same that enables workers to distinguish between queen-and worker-laid eggs. Despite their viability, the latter are destroyed by workers when given a choice between both types. Queen-and worker-laid eggs differ in their surface hydrocarbons in a way similar to the way fertile queens differ from workers in the composition of their cuticular hydrocarbons. When we transferred hydrocarbons from the queen cuticle to worker-laid eggs, the destruction of those eggs was significantly mitigated. We conclude that queen-derived hydrocarbon labels inform workers about the presence of a fertile queen and thereby regulate worker reproduction.

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Section: Discussionmentioning

confidence: 99%

Surface hydrocarbons of queen eggs regulate worker reproduction in a social insect

Endler

Liebig

Schmitt

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

258

219

View full text Add to dashboard Cite

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“…Workers probably establish the source of an egg on the basis of substances that are deposited on the egg's surface when it is laid (Katzav-Gozansky et al, 2001). The queen's eggs have a more complex hydrocarbon coating than worker-laid eggs, and acetates of fatty alcohols, alkenes, and monomethylalkanes are characteristic to eggs that are laid by a queen (Katzav-Gozansky et al, 2003).…”

Section: Killing Of Developing Brood-mentioning

confidence: 99%

Order, Disorder, Death: Lessons from a Superorganism

Amdam¹,

Seehuus²

2006

Advances in Cancer Research

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Animal models contribute to the understanding of molecular mechanism of cancer, revealing complex roles of altered cellular-signaling networks and deficient surveillance systems. Analogous pathologies are documented in an unconventional model organism that receives attention in research on systems theory, evolution, and aging. The honeybee (Apis mellifera) colony is an advanced integrative unit, a "superorganism" in which order is controlled via complex signaling cascades and surveillance schemes. A facultatively sterile caste, the workers, regulates patterns of growth, differentiation, homeostasis, and death. Workers differentiate into temporal phenotypes in response to dynamic social cues; chemosensory signals that can translate into dramatic physiological responses, including programmed cell death. Temporal worker forms function together, and effectively identify and terminate abnormal colony members ranging from embryos to adults. As long as this regulatory system is operational at a colony level, the unit survives and propagates. However, if the worker phenotypes that collectively govern order become too few or change into malignant forms that bypass control mechanisms to replicate aberrantly; order is replaced by disorder that ultimately leads to the destruction of the society. In this chapter we describe fundamental properties of honeybee social organization, and explore conditions that lead to states of disorder. Our hope is that this chapter will be an inspirational source for ongoing and future work in the field of cancer research.

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“…To date, although several studies have examined morphological change (2,26,27) and gene expression (25,28,29) during the period of embryogenesis in the honey bee, only a few works report on the preliminary results of the unraveling molecular underpinnings of worker (30) and drone (31) embryogenesis at the proteomic level, identifying only 107 proteins. MS-based proteomics is the primary technology that enables a system-wide view of proteomes and their changes.…”

mentioning

confidence: 99%

In-depth Proteomics Characterization of Embryogenesis of the Honey Bee Worker (Apis mellifera ligustica)

Mao

Han

et al. 2014

Molecular & Cellular Proteomics

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Identifying proteome changes of honey bee embryogenesis is of prime importance for unraveling the molecular mechanisms that they underlie. However, many proteomic changes during the embryonic period are not well characterized. We analyzed the proteomic alterations over the complete time course of honey bee worker embryogenesis at 24, 48, and 72 h of age, using mass spectrometry-based proteomics, label-free quantitation, and bioinformatics. Of the 1460 proteins identified the embryo of all three ages, the core proteome (proteins shared by the embryos of all three ages, accounting for 40%) was mainly involved in protein synthesis, metabolic energy, development, and molecular transporter, which indicates their centrality in driving embryogenesis. However, embryos at different developmental stages have their own specific proteome and pathway signatures to coordinate and modulate developmental events. The young embryos (<24 h) stronger expression of proteins related to nutrition storage and nucleic acid metabolism may correlate with the cell proliferation occurring at this stage. The middle aged embryos (24 -48 h) enhanced expression of proteins associated with cell cycle control, transporters, antioxidant activity, and the cytoskeleton suggest their roles to support rudimentary organogenesis. Among these proteins, the biological pathways of aminoacyl-tRNA biosynthesis, ␤-alanine metabolism, and protein export are intensively activated in the embryos of middle age. The old embryos (48 -72 h) elevated expression of proteins implicated in fatty acid metabolism and morphogenesis indicate their functionality for the formation and development of organs and dorsal closure, in which the biological pathways of fatty acid metabolism and RNA transport are highly activated. These findings add novel understanding to the molecular details of honey bee embryogenesis, in which the programmed activation of the proteome matches with the physiological transition observed during embryogenesis. The identified biological pathways and key node proteins allow for further functional analysis and genetic manipulation for both the honey bee embryos and other eusocial insects. Molecular & Cellular Proteomics 13:

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Ultrastructural and chemical characterization of egg surface of honeybee worker and queen-laid eggs

Cited by 33 publications

References 28 publications

Surface hydrocarbons of queen eggs regulate worker reproduction in a social insect

Surface hydrocarbons of queen eggs regulate worker reproduction in a social insect

Order, Disorder, Death: Lessons from a Superorganism

In-depth Proteomics Characterization of Embryogenesis of the Honey Bee Worker (Apis mellifera ligustica)

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