Ultrastructural and histomorphometric alterations of rat jaw bones after experimental induction of lathyrism

Rouby, Dalia Hussein El; Bashir, Maha Hassan; Korany, Nahed Sedky

doi:10.1016/j.archoralbio.2008.04.008

Cited by 11 publications

(14 citation statements)

References 31 publications

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“…The collagen disorganization in this study is consistent with the results of EL‐Rouby et al. (20) who examined the rat jaw bones after experimental induction of lathyrism and reported gross alterations in the micro‐architecture of the collagen fibers in the bone of lathyritic rats. It is worth mentioning that as collagen structure and mineralization are closely associated, it is likely that when changes occur in one, both are affected (29, 30).…”

Section: Discussionsupporting

confidence: 92%

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The effect of lathyrism on dentin structure of the rat incisors: a morphometric and scanning electron microscopic investigation

Rouby

Bashir

Korany

2010

J Oral Pathology Medicine

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Section: Discussionsupporting

confidence: 92%

“…(19) and El‐Rouby et al. (20) who detected a noticeable decrease in the level of alveolar bone and in the area percentage of bones in lathyritic rats as compared with control.…”

Section: Discussionmentioning

confidence: 93%

The effect of lathyrism on dentin structure of the rat incisors: a morphometric and scanning electron microscopic investigation

Rouby

Bashir

Korany

2010

J Oral Pathology Medicine

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“…The dose‐dependent reduction in weight gain and bone cortical area with BAPN treatment (Table ) support there being less new (BAPN‐treated) cortical tissue formed at the highest BAPN dose compared with the other groups. It is also possible that BAPN increased osteoclast number or activity, contributing to reduced bone size . Regardless of the cellular mechanism, the lack of effect on strength and toughness at the highest BAPN dose could be explained by a reduction or change in distribution of the cross‐link–deficient tissue.…”

Section: Discussionmentioning

confidence: 99%

“…It is also possible that BAPN increased osteoclast number or activity, contributing to reduced bone size. (45) Regardless of the cellular mechanism, the lack of effect on strength and toughness at the highest BAPN dose could be explained by a reduction or change in distribution of the cross-link-deficient tissue. The potential for a volume averaging of preexisting and experimentally treated tissue to influence experimental measurements is an often overlooked confounding factor with broad implications in skeletal research.…”

Section: Discussionmentioning

confidence: 99%

Bone Fracture Toughness and Strength Correlate With Collagen Cross-Link Maturity in a Dose-Controlled Lathyrism Mouse Model

McNerny

Gong

Morris

et al. 2014

Journal of Bone and Mineral Research

121

117

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Collagen cross-linking is altered in many diseases of bone, and enzymatic collagen cross-links are important to bone quality as evidenced by losses of strength following lysyl oxidase inhibition (lathyrism). We hypothesized that cross-links also contribute directly to bone fracture toughness. A mouse model of lathyrism using subcutaneous injection of up to 500mg/kg β-aminopropionitrile (BAPN) was developed and characterized (60 animals across 4 dosage groups). Three weeks of 150 or 350 mg/kg BAPN treatment in young growing mice significantly reduced cortical bone fracture toughness, strength, and pyridinoline cross-link content. Ratios reflecting relative cross-link maturity were positive regressors of fracture toughness (HP/[DHLNL+HLNL] r2=0.208, p<0.05; [HP+LP]/[DHNL+HLNL] r2=0.196, p<0.1), whereas quantities of mature pyridinoline cross-links were significant positive regressors of tissue strength (lysyl pyridinoline r2=0.159, p=0.014; hydroxylysyl pyridinoline r2=0.112, p<0.05). Immature and pyrrole cross-links, which were not significantly reduced by BAPN, did not correlate with mechanical properties. The effect of BAPN treatment on mechanical properties was dose specific, with the greatest impact found at the intermediate (350mg/kg) dose. Calcein labeling was used to define locations of new bone formation, allowing for the identification of regions of normally cross-linked (preexisting) and BAPN treated (newly formed, cross-link-deficient) bone. Raman spectroscopy revealed spatial differences due to relative tissue age and effects of cross-link inhibition. Newly deposited tissues had lower mineral/matrix, carbonate/phosphate and Amide I cross-link (matrix maturity) ratios compared to preexisting tissues. BAPN treatment did not affect mineral measures, but significantly increased the cross-link (matrix maturity) ratio compared to newly formed control tissue. Our study reveals that spatially localized effects of short term BAPN cross-link inhibition can alter the whole bone collagen cross-link profile to a measureable degree, and this cross-link profile correlates with bone fracture toughness and strength. Thus, cross-link profile perturbations associated with bone disease may provide insight into bone mechanical quality and fracture risk.

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“…9 The sweet pea (Lathyrus odoratus) contains b-aminopropionitrile (BAPN), 10 which irreversibly binds to the LOX active site, 11 and is a commonly used lathyrogen to experimentally replicate osteolathyrism. [12][13][14][15] Understanding the implications of reduced enzymatic crosslinking is important because crosslinks are reduced with aging and other diseases such as diabetes and osteoporosis. 16 Therefore, treatment with BAPN can serve as a model of osteolathyrism and further our understanding of how nanoscale changes in collagen impact bone strength and fracture resistance.…”

Section: Introductionmentioning

confidence: 99%

Exercise prevents β-aminopropionitrile-induced morphological changes to type I collagen in murine bone

Hammond¹,

Wallace²

2015

BoneKEy Reports

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This study evaluated the effects of reduced enzymatic crosslinking, exercise and the ability of exercise to prevent the deleterious impact of reduced crosslinking on collagen D-spacing. Eight-week-old female mice were divided into four weight-matched groups receiving daily injections of either phosphate-buffered saline (PBS) or 300 mg kg À 1 b-aminopropionitrile (BAPN) while undergoing normal cage activity (Sed) or 30 min per day of treadmill exercise (Ex) for 21 consecutive days. BAPN caused a downward shift in the D-spacing distribution in Sed BAPN compared with Sed PBS (Po0.001) but not in Ex BAPN (P ¼ 0.429), indicating that exercise can prevent changes in collagen morphology caused by BAPN. Exercise had no effect on D-spacing in PBS control mice (P ¼ 0.726), which suggests that exercise-induced increases in lysyl oxidase may be a possible mechanism for preventing BAPN-induced changes in D-spacing. The D-spacing changes were accompanied by an increase in mineral crystallinity/maturity due to the main effect of BAPN (P ¼ 0.016). However, no changes in nanoindentation, reference point indentation or other Raman spectroscopy parameters were observed. The ability of exercise to rescue BAPN-driven changes in collagen morphology necessitates further research into the use of mechanical stimulation as a preventative therapy for collagen-based diseases.

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Ultrastructural and histomorphometric alterations of rat jaw bones after experimental induction of lathyrism

Cited by 11 publications

References 31 publications

The effect of lathyrism on dentin structure of the rat incisors: a morphometric and scanning electron microscopic investigation

The effect of lathyrism on dentin structure of the rat incisors: a morphometric and scanning electron microscopic investigation

Bone Fracture Toughness and Strength Correlate With Collagen Cross-Link Maturity in a Dose-Controlled Lathyrism Mouse Model

Exercise prevents β-aminopropionitrile-induced morphological changes to type I collagen in murine bone

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