Ultrastructural and immunohistochemical study of hepatic stellate cells over the course of infectious viral fibrosis and cirrhosis of the liver

Nepomnyashchikh, G. I.; Айдагулова, С. В.; Nepomnyashchikh, D. L.; Kapustina, V. I.; Postnikova, Olga A.

doi:10.1007/s10517-006-0462-z

Cited by 9 publications

(9 citation statements)

References 14 publications

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“…HSCs treated with PSCs did not contain lipid droplets compared to HSC controls. The number of lipid-containing HSCs decreased signi cantly during the transformation phase of liver cirrhosis, indicating that the cells underwent brogenic transformation [18]. All features resulting from treatment of HSCs with PSCs, including multiplication or disappearance of the nucleus, enlargement of the Golgi apparatus, changes in the cytoplasm, and nally, transformation of cytoplasm into autophagosomes, indicate brogenic transformation compared to the HSC controls.…”

Section: Discussionmentioning

confidence: 99%

Structural Changes and Detection of Liver Fibrosis–Related Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate Cells

Cao

Shamsan

Jiang

et al. 2021

Preprint

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BackgroundEchinococcus multilocularis is a causative agent of human alveolar echinococcosis (AE). AE leads to cirrhosis in several organs, such as the liver, triggering severe conditions, including hepatic failure and encephalopathy. The main purpose of this study is to explore the interaction between treated hepatic stellate cells and AE-protoscoleces (AE-PSCs). The results of this study will be provided experimental basis for revealing the mechanisms of hepatic fibrosis after AE infection.MethodsWe investigated the role of alveolar echinococcosis-protoscoleces (AE-PSCs) in liver fibrosis and structural changes and liver fibrosis-related protein expression in a coculture of PSCs and human hepatic stellate cells (HSCs). Structural changes were detected by transmission electron microscopy, whereas liver fibrosis-related proteins, collagen I, alpha-smooth muscle actin, and osteopontin levels were measured by western blotting and enzyme-linked immunosorbent assay. ResultsPSCs exhibited morphological changes, specifically changes in shape, and showed slight changes in the cytoplasmic membrane, whereas structural modifications were observed in HSCs. Additionally, western blotting and enzyme-linked immunosorbent assay revealed that PSCs treated in vitro with HSC-LX2 showed significantly increased collagen-Ⅰ, α-smooth muscle actin, and osteopontin expression levels after 3–4 days of incubation in a coculture system. AE-PSCs induced liver fibrosis by inducing extracellular matrix expression and HSC activation.ConclusionsThese results provide insight into the pathogenesis of echinococcosis- induced hepatic fibrosis and introduce therapeutic targets and diagnostic criteria for managing echinococcosis-dependent liver fibrosis.

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Section: Discussionmentioning

confidence: 99%

Structural Changes and Detection of Liver Fibrosis–Related Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate Cells

Cao

Shamsan

Jiang

et al. 2021

Preprint

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“…In the present study, HSCs exposed to PSCs did not contain lipid droplets, whereas the HSC controls did. The number of lipid-containing HSCs decreased significantly during the transformative phases of liver fibrosis, which indicated that they underwent fibrogenic transformation [ 24 ]. All of the features resulting from the exposure of the HSCs to the PSCs, including the division or malformation and irregularity of the nucleus, and the microvilli on the cell membrane increasing and the mitochondria swelling are indicative of apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…Fasciola hepatica and Dicrocoelium dendriticum . HSCs play an important role in the development of fibrosis and other stages of cirrhosis [ 24 , 30 ]. TGF-β plays a role in the differentiation of HSCs; it is the main factor that activates these cells, which leads to collagen deposition [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate cells

et al. 2021

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Background Echinococcus multilocularis is the causative agent of human hepatic alveolar echinococcosis (AE). AE can cause damage to several organs, primarily the liver, and have severe outcomes, such as hepatic failure and encephalopathy. The main purpose of this study was to explore the interactions between hepatic stellate cells (HSCs) and E. multilocularis protoscoleces (PSCs). The results of this study provide an experimental basis for further examination of the pathogenesis of hepatic fibrosis due to AE infection. Methods We investigated the role of Echinococcus multilocularis (Echinococcus genus) PSCs in hepatic fibrosis by examining structural changes and measuring hepatic fibrosis-related protein levels in cocultures of PSCs and human HSCs. Structural changes were detected by transmission electron microscopy (TEM), and levels of the hepatic fibrosis-related proteins collagen I (Col-I), alpha-smooth muscle actin (α-SMA) and osteopontin (OPN) were measured by western blotting and enzyme-linked immunosorbent assay (ELISA). Results Under coculture (1) both PSCs and HSCs exhibited morphological changes, as observed by TEM; (2) Col-I, α-SMA, and OPN expression levels, which were determined by western blotting and ELISA, significantly increased after 3 days of incubation. Conclusions The results of this study provide insights into the molecular mechanisms of AE-induced hepatic fibrosis. Graphical abstract

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“…A number of morphological features of HSCs in fibrotic livers were studied by electron microscopy [ 115 , 116 ]. In both studies, liver biopsies of human patients with chronic hepatitis C were imaged.…”

Section: Hepatic Stellate Cells In Healthy and Diseased Livermentioning

confidence: 99%

“…In livers with increasing fibrosis severity scores, numbers of LD-containing HSCs decreased [ 116 ]. Even in the most pronounced fibrotic stages, LDs did not disappear completely [ 115 ]. In addition, a comparison of HSCs isolated by two different FACS (Fluorescent Activated Cell Sorting) protocols underlined the relation between LD size and activation status: retinoid autofluorescence-sorted cells—associated with a quiescent state—display larger LDs than cells sorted by GFP under a collagen promotor—typically linked to a more activated state [ 100 ].…”

Section: Hepatic Stellate Cells In Healthy and Diseased Livermentioning

confidence: 99%

Playing Jekyll and Hyde—The Dual Role of Lipids in Fatty Liver Disease

Molenaar

Penning

Helms

2020

Cells

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Lipids play Jekyll and Hyde in the liver. On the one hand, the lipid-laden status of hepatic stellate cells is a hallmark of healthy liver. On the other hand, the opposite is true for lipid-laden hepatocytes—they obstruct liver function. Neglected lipid accumulation in hepatocytes can progress into hepatic fibrosis, a condition induced by the activation of stellate cells. In their resting state, these cells store substantial quantities of fat-soluble vitamin A (retinyl esters) in large lipid droplets. During activation, these lipid organelles are gradually degraded. Hence, treatment of fatty liver disease is treading a tightrope—unsophisticated targeting of hepatic lipid accumulation might trigger problematic side effects on stellate cells. Therefore, it is of great importance to gain more insight into the highly dynamic lipid metabolism of hepatocytes and stellate cells in both quiescent and activated states. In this review, part of the special issue entitled “Cellular and Molecular Mechanisms underlying the Pathogenesis of Hepatic Fibrosis 2020”, we discuss current and highly versatile aspects of neutral lipid metabolism in the pathogenesis of non-alcoholic fatty liver disease (NAFLD).

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Ultrastructural and immunohistochemical study of hepatic stellate cells over the course of infectious viral fibrosis and cirrhosis of the liver

Cited by 9 publications

References 14 publications

Structural Changes and Detection of Liver Fibrosis–Related Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate Cells

Structural Changes and Detection of Liver Fibrosis–Related Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate Cells

Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate cells

Playing Jekyll and Hyde—The Dual Role of Lipids in Fatty Liver Disease

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