Ultrastructural and molecular characterization of Nosema alticae sp. nov. (Microsporidia: Nosematidae), pathogen of the flea beetle, Altica hampei Allard, 1867 (Coleoptera: Chrysomelidae)

Yıldırım, Hilal; Bekircan, Çağrı

doi:10.1016/j.jip.2019.107302

Cited by 8 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, the samples which were collected commercially produced members from greenhouses lands or hives, had been found dead when collected. Members of B. terrestris morphologically identified according to Mauss (1994) and for wet mount preparation samples were dissected with Ringer's solution and examined under the light microscope (Tosun, 2020;Yıldırım & Bekircan, 2020). Infection positive slides were photographed using Zeiss AXIO microscope equipped with an Axicam ERc5s digital camera.…”

Section: Specimen Collection and Light Microscopical Observationmentioning

confidence: 99%

Molecular Characterization of the Vairimorpha (Nosema) ceranae Infection from Bombus terrestris (Linnaeus, 1758) (Hymenoptera: Apidae) in Turkey

Tosun¹,

Bekircan²

2021

jers

Self Cite

View full text Add to dashboard Cite

The current study aimed to determine the natural Microsporidium pathogen of the Bombus terrestris L. (Hymenoptera: Apidae) in Turkey (Mersin, Antalya, Muğla, İzmir, Aydın). During 2019 and 2020, the commercial and wild populations of B. terrestris were investigated in this survey. In the studies, natural microsporidiosis was detected in commercial B. terrestris populations. Fresh oval spores were measured as 4.91 ± 0.48 (6.12 -3.73) µm in length and 2.54 ± 0.31 (3.27 -1.88) µm in width (n=60). Both SSU rRNA and RPB1 gene sequences of the current microsporidium were top hits with the Vairimorpha (Nosema) ceranae isolates. While the SSU rRNA gene sequence matched with the Vairimorpha ceranae clone NCS44 (LC510228) isolated from the Apis cerana japonica at 99.24% identity (100% coverage), the RPB1 gene sequence was matched with the Vairimorpha ceranae isolate 1994 (KJ473287) at 99.02% identity (100% coverage). Based on the light microscopy and molecular phylogeny the current microsporidium was a new isolate of the V. ceranae and named here in as Vairimorpha ceranae Tr-07.

show abstract

Section: Specimen Collection and Light Microscopical Observationmentioning

confidence: 99%

Molecular Characterization of the Vairimorpha (Nosema) ceranae Infection from Bombus terrestris (Linnaeus, 1758) (Hymenoptera: Apidae) in Turkey

Tosun¹,

Bekircan²

2021

jers

Self Cite

View full text Add to dashboard Cite

show abstract

“…Purified spores were placed in a 0.5 ml microfuge tube with equal volumes 0.3% hydrogen peroxide (H 2 O 2 ) and kept at room temperature for 15 206143) was used. The 18F/1537R primer set was used to amplify the SSU rRNA gene (18F/1537R: 5 ′ -CACCA GGTTG ATTCT GCC-3 ′ /5 ′ -TTATG ATCCT GCTAA TGGTT C-3 ′ ) and the primers for the RPB1 gene were newly designed (Yıldırım and Bekircan, 2020). For sequencing, bidirectional readings were made and after the necessary examinations, a consensus sequence was established and loaded GenBank.…”

Section: Molecular Studiesmentioning

confidence: 99%

A new isolate ofNosema fumiferanae(Microsporidia: Nosematidae) from the date mothApomyelois(Ectomyelois)ceratoniae, Zeller, 1839 (Lepidoptera: Pyralidae)

Tosun¹

2020

Parasitology

View full text Add to dashboard Cite

In this study, a microsporidian pathogen of the date moth (Apomyelois (Ectomyelois) ceratoniae, Zeller, 1839) also known as the carob moth, is described based on light microscopy, ultrastructural characteristics and comparative molecular analysis. The pathogen infects the gut and hemolymph of A. ceratoniae. All development stages are in direct contact with the host cell cytoplasm. Fresh spores with nuclei arranged in a diplokaryon are oval and measured 3.29 ± 0.23 μm (4.18–3.03 μm, n = 200) in length and 1.91 ± 0.23 μm (2.98–1.66 μm, n = 200) in width. Spores stained with Giemsa's stain measured 3.11 ± 0.31 μm (3.72–2.41 μm, n = 150) in length and 1.76 ± 0.23 μm (2.16–1.25 μm, n = 150) in width. Spores have an isofilar polar filament with 10-12 coils. An 1110 bp long alignment of the current microsporidium showed an SSU rRNA gene difference of only 0.0009, corresponding to >99.91% sequence similarity with Nosema fumiferanae, while RPB1 gene sequences were 98.03% similar within an alignment of 969 bp. All morphological, ultrastructural and molecular features indicate that the microsporidian pathogen of A. ceratoniae is the new isolate of the N. fumiferanae and is named here as Nosema fumiferanae TY61.

show abstract

“…In recent years, other combat techniques such as microbial control have attracted attention in pest control. Entomopathogenic organisms that cause infection in pests such as E. kuehniella frequently recorded in the literature, and there is increasing interest in isolating and identifying these organisms [6,7,8,9,10,11].…”

Section: Introductionmentioning

confidence: 99%

A New Microsporidian Pathogen of the Mediterranean Flour Moth, Ephestiae kuehniella (Zeller) (Lepidoptera: Pyralidae)

Bekircan¹,

Tosun²

2021

Biological Diversity and Conservation

View full text Add to dashboard Cite

In this study, a new Vairimorpha isolate was defined from Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) in Turkey (Malatya). Infection was determined in the host's hindgut, both larval and adult stages. Two types spore originated from the polymorphic sporulation was detected during the observations. While the binucleate ovocylindrical fresh spores were measured 4.38 ± 0.31 μm (5.09-3.77 μm, n = 100) in length and 2.80 ± 0.18 μm (3.21-2.43 μm, n=100) in width, the ovoid meiospores were measured as 3.50 ± 0.46 μm (4.98-2.51 μm, n = 100) in length and 2.02 ± 0.28 μm (2.94-1.50 μm, n=100) in width. The multilocus genetic analyses based on SSU rRNA and RPB1 genes were carried for determining the phylogenetic status of the current microsporidium. In ML and BI trees, the current microsporidium was separated into a different branch from V. necatrix strains within the Vairimorpha clade

show abstract

Ultrastructural and molecular characterization of Nosema alticae sp. nov. (Microsporidia: Nosematidae), pathogen of the flea beetle, Altica hampei Allard, 1867 (Coleoptera: Chrysomelidae)

Cited by 8 publications

References 20 publications

Molecular Characterization of the Vairimorpha (Nosema) ceranae Infection from Bombus terrestris (Linnaeus, 1758) (Hymenoptera: Apidae) in Turkey

Molecular Characterization of the Vairimorpha (Nosema) ceranae Infection from Bombus terrestris (Linnaeus, 1758) (Hymenoptera: Apidae) in Turkey

A new isolate ofNosema fumiferanae(Microsporidia: Nosematidae) from the date mothApomyelois(Ectomyelois)ceratoniae, Zeller, 1839 (Lepidoptera: Pyralidae)

A New Microsporidian Pathogen of the Mediterranean Flour Moth, Ephestiae kuehniella (Zeller) (Lepidoptera: Pyralidae)

Contact Info

Product

Resources

About