Ultrastructural and Stereological Study of the Liver in Chronic Mixed HCV + HBV Infection

Postnikova, Olga A.; Айдагулова, С. В.; Nepomnyashchikh, D. L.; Nokhrina, Zh. V.; Vinogradova, Ekaterina V.; Karavaeva, Yu. Yu.

doi:10.1007/s10517-012-1626-7

Cited by 2 publications

(4 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Also, destruction of the cytoplasmic organelles can be corresponded to the infectious process activity. 14 A trend to an increase in the hepatocyte volume was traced in HBV-related HCC group in cases with high activity of the infectious process in comparison with the parameter in only HCC patients without infectious activity. It may be due to high volume/volume proportion of the protein synthesizing compartment and mitochondrial organelles vs hepatocyte cytoplasm.…”

Section: Discussionmentioning

confidence: 89%

“…But, on the other hand, qualitative findings by transmission electron microscopy showed that the number of organelles were reduced and played an additional role in liver damage. 14 According to studies, liver and hepatocyte hypertrophy were caused by increasing in the flow of inflammatory factors to the liver after prolonged chronic infection such as HBV. It also is interesting to observe the relationships between the liver and its compartment units.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, Postnikova et al 14 studied the ultrastructural and stereological changes of the liver in chronic mixed HCV+HBV infection in humans. They showed changes in the hepatocyte ultrastructure of different degree by electron microscopy.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Study of liver in HBV-related hepatocellular carcinoma: Stereology shows quantitative differences in liver structure

2018

View full text Add to dashboard Cite

Hepatocellular carcinoma (HCC) is one of the main consequences of liver chronic disease. Hepatocellular carcinoma-related changes may be seen in patients with chronic hepatitis B. The aim of the current study was to quantitate liver tissue elements by stereological technique in patients with hepatitis B-related cancer and compare the results with control and only hepatitis B group. Needle liver biopsies from 40 patients with only chronic hepatitis B infection, from 41 patients with only early hepatocellular carcinoma, from 40 patients with early hepatitis B-related cancer and 30 healthy subjects (control group) were analyzed by stereological method using systematic uniform random sampling method. Haematoxylin and eosin stained sections were used. The numerical density of hepatocytes, hepatocyte volume, numerical density of Kupffer cells, volume density of the connective tissue in the portal space, and volume density of the connective tissue were assessed. Quantitative analysis of liver samples indicated that there were statistically significant differences in the numerical density of hepatocytes, hepatocyte volume, numerical density of Kupffer cells, volume density of the connective tissue in the portal space, and volume density of the connective tissue between control and hepatitis B-related cancer and hepatitis B groups. Quantitative, stereological technique is simple and reliable for evaluating HCC in chronic hepatitis B. It is useful for assessing the liver tissue parameters. Stereology is recommended for the diagnosis of people prone to cancer in patients with chronic hepatitis B.

show abstract

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Study of liver in HBV-related hepatocellular carcinoma: Stereology shows quantitative differences in liver structure

2018

View full text Add to dashboard Cite

show abstract

“…High serum HCV levels preand postliver transplant were associated with cholestatic fibrosis, a severe form of hepatitis [16]. EM of HCV RNA-positive liver biopsies showed that the hepatocytes would undergo vacuolation and lysis of membrane organelles, degeneration of mitochondria, dilatation of cytoplasmic reticulum tubules, and formation of an appreciable amount of large polymorphic secondary phagosomes [17]. Kasprzak et al summed up the data on nuclear lesions of liver biopsies with chronic HCV infection, involving swelling, altered shape, hyperchromasia, disturbed nuclear chromatin structure; and the nuclear lesions always occurred in the cells with changes in rough ER within long tubular structures or branching fibrils inside [18].…”

Section: Bbhcv Infection Activates Anti-apoptotic Proteinsmentioning

confidence: 99%

Hepatitis C virus infection induces endoplasmic reticulum stress and apoptosis in human fetal liver stem cells

Guo

Liu²,

Yang³

et al. 2019

The Journal of Pathology

View full text Add to dashboard Cite

The cellular mechanisms by which hepatitis C virus (HCV) replication might mediate cytopathic effects are controversial and not entirely clear. In this study, we found that blood-borne HCV (bbHCV) infection could lead to endoplasmic reticulum (ER)-stress and mitochondria-related/caspase-dependent apoptosis at the early stages of infection based on use of the highly efficient bbHCV cell culture model established previously. Sections of bbHCV-infected human fetal liver stem cells (hFLSCs) revealed convolution and nonlinear ER, cell vacuolization, swelling of mitochondria, and numerous double membrane vesicles (DMVs). The percentage of apoptotic hFLSCs infected by bbHCV reached 29.8% at 16 h postinfection, and the amount of cytochrome c increased remarkably in the cytosolic protein fraction. However, over time, apoptosis was inhibited due to the activation of NF-B. The expression of NF-B-p65, Bcl-xL, XIAP, and c-FLIPL in hFLSCs was increased significantly 24 h after in infection by bbHCV. The accelerated cell death cycles involving apoptosis, regeneration and repair by bbHCV infection might give rise to the development of cirrhosis, and ultimately to hepatocellular carcinogenesis.We then explored the role ER stress plays in cells apoptosis. The expression of ER stress-related proteins, GRP78 and phosphorylated eIF2-alpha were assessed in bbHCV-infected cells. The overexpression of GRP78 and phosphorylated eIF2-alpha strongly suggested that bbHCV infection could induce ER stress as an early cell DiscussionHCV infection induced CPE is part of the infection process. Whether the established infection model could simulate and undergo CPE similar to lesions observed in hepatitis patients could be a measure of the model's success. The presentation of HCV-induced CPE is vital in cell culture systems, and could contribute to the

show abstract

Ultrastructural and Stereological Study of the Liver in Chronic Mixed HCV + HBV Infection

Cited by 2 publications

References 10 publications

Study of liver in HBV-related hepatocellular carcinoma: Stereology shows quantitative differences in liver structure

Study of liver in HBV-related hepatocellular carcinoma: Stereology shows quantitative differences in liver structure

Hepatitis C virus infection induces endoplasmic reticulum stress and apoptosis in human fetal liver stem cells

Contact Info

Product

Resources

About