Ultrastructural aspects of fertile and sterile cysts of Echinococcus granulosus developed in hosts of different species

Bortoletti, G.; Ferretti, G

doi:10.1016/0020-7519(78)90058-9

Cited by 52 publications

(27 citation statements)

References 9 publications

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“…Cysts were processed as described before [Galindo et al, 2003; Martinez et al, 2005; Paredes et al, 2007]. Macroscopic observation was used to define as fertile a cyst presenting free protoscoleces in the hydatid fluid, a whitish color and a thick laminar layer [Bortoletti and Ferretti, 1978]. They were confirmed under light microscopy by the presence of buds and both grown protoscoleces attached to the germinal layer and free protoscoleces in the hydatid fluid.…”

Section: Methodsmentioning

confidence: 99%

Bovine (Bos taurus) humoral immune response against Echinococcus granulosus and hydatid cyst infertility

Paredes

Godoy

Rodríguez

et al. 2011

J of Cellular Biochemistry

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Echinococcus granulosus, the agent of hydatid disease, presents an indirect life cycle, with canines (mainly dogs) as definitive hosts, and herbivores and human as intermediary ones. In intermediary hosts fertile and infertile cysts develop, but only the first ones develop protoscoleces, the parasite form infective to definitive hosts. We report the presence of bovine IgGs in the germinal layer from infertile cysts (GLIC), in an order of magnitude greater than in the germinal layer from fertile cysts (GLFC). When extracted with salt solutions, bovine IgGs from GLIC are associated with low or with high affinity (most likely corresponding to non specific and antigen specific antibodies, respectively). Specific IgGs penetrate both the cells of the germinal layer and HeLa cultured cells and recognize parasitic proteins. These results, taken together with previous ones from our laboratory, showing induction of apoptosis in the germinal layer of infertile hydatid cysts, provide the first coherent explanation of the infertility process. They also offer the possibility of identifying the parasite antigens recognized, as possible targets for immune modulation.

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Section: Methodsmentioning

confidence: 99%

Bovine (Bos taurus) humoral immune response against Echinococcus granulosus and hydatid cyst infertility

Paredes

Godoy

Rodríguez

et al. 2011

J of Cellular Biochemistry

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“…Electron micrographs of the E. granulosus HCW have consistently shown that the LL is formed by two ultrastructural elements: a predominant microfibrillar matrix and the granular bodies [Morseth, 1967;Bortoletti and Ferretti, 1978;Richards et al, 1983a;Rogan and Richards, 1989]. The microfibrillar matrix stains positive for carbohydrates, and is thus thought to be made up from the glycoconjugates that account for the bulk of the LL chemically [Richards et al, 1983a].…”

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confidence: 95%

Unique precipitation and exocytosis of a calcium salt of myo‐inositol hexakisphosphate in larval Echinococcus granulosus

Irigoı́n¹,

Casaravilla²,

Iborra³

et al. 2004

J of Cellular Biochemistry

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The ubiquitous intracellular molecule myo-inositol hexakisphosphate (IP6) is present extracellularly in the hydatid cyst wall (HCW) of the parasitic cestode Echinococcus granulosus. This study shows that extracellular IP6 is present as its solid calcium salt, in the form of deposits that are observed, at the ultrastructural level, as naturally electron dense granules some tens of nanometers in diameter. The presence of a calcium salt of IP6 in these structures was determined by two different electron microscopy techniques: (i) the analysis of the spatial distribution of phosphorus and calcium in the outer, acellular layer of the HCW (the laminated layer, LL) through electron energy loss spectroscopy, and (ii) the observation, by transmission electron microscopy, of HCW that were selectively depleted of IP6 by treatment with EGTA or phytase, an enzyme that catalyses the dephosphorylation of IP6. The deposits of the IP6-Ca(II) salt are also observed inside membrane vesicles in cells of the germinal layer (the inner, cellular layer of the HCW), indicating that IP6 precipitates with calcium within a cellular vesicular compartment and is then secreted to the LL. Thus, much as in plants (that produce vesicular IP6 deposits), the existence of transporters for IP6 or its precursors in internal membranes is needed to explain the compound's cellular localisation in E. granulosus.

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“…For all these reasons, we believe that these cells may correspond to cells located near the tegument, a site of DNA synthesis that is associated to the in vitro larval development of protoscoleces (Galindo et al 2008). Structure and organization of germinal membrane is similar to that of adult cestode tegument (Bortoletti and Ferretti 1978). This same structure and organization may be found not only in pe.…”

Section: Discussionmentioning

confidence: 82%

“…In Caenorhabditis elegans, for example, this mechanism seems to be involved in worm maturation (Hedgecock and White 1985). E. granulosus protoscoleces maturation, however, is not associated with an increased chromosome number or DNA content (Bortoletti and Ferretti 1978). Furthermore, considering the large number of cells growing very close together, forming a syncytium, it is possible that cells could be cycling.…”

Section: Discussionmentioning

confidence: 97%

Morphological and biological characterization of cell line developed from bovine Echinococcus granulosus

Echeverría

Isolabella

González

et al. 2010

In Vitro Cell.Dev.Biol.-Animal

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The taeniid tapeworm Echinococcus granulosus is the causative agent of echinococcal disease, a major zoonosis with worldwide distribution. Several efforts to establish an in vitro model of E. granulosus have been undertaken; however, many of them have been designed for Echinococcus multilocularis. In the present study, we have described and characterized a stable cell line obtained from E. granulosus bovine protoscoleces maintained 3 yr in vitro. Growth characterization, morphology by light, fluorescent and electronic microscopy, and karyotyping were carried out. Cell culture origin was confirmed by immunofluorescent detection of AgB4 antigen and by PCR for the mitochondrial cytochrome c-oxidase subunit 1 (DCO1) gene. Cells seeded in agarose biphasic culture resembled a cystic structure, similar to the one formed in secondary hosts. This cell line could be a useful tool to research equinococcal behavior, allowing additional physiological and pharmacological studies, such as the effect of growth factors, nutrients, and antiparasitic drugs on cell viability and growth and on cyst formation.

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Ultrastructural aspects of fertile and sterile cysts of Echinococcus granulosus developed in hosts of different species

Cited by 52 publications

References 9 publications

Bovine (Bos taurus) humoral immune response against Echinococcus granulosus and hydatid cyst infertility

Bovine (Bos taurus) humoral immune response against Echinococcus granulosus and hydatid cyst infertility

Unique precipitation and exocytosis of a calcium salt of myo‐inositol hexakisphosphate in larval Echinococcus granulosus

Morphological and biological characterization of cell line developed from bovine Echinococcus granulosus

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