Ultrastructural changes associated to the neuroendocrine transdifferentiation of the lung adenocarcinoma cell line A549

Mendieta, Irasema; Rodríguez-Nieto, Maricela; Núñez-Anita, Rosa Elvira; Menchaca-Arredondo, Jorge Luis; Garcı́a-Alcocer, Guadalupe; Berumen, Laura C.

doi:10.1016/j.acthis.2021.151797

Cited by 10 publications

(5 citation statements)

References 48 publications

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“…The CMF-ENZ consisting of cellulose microfibrils produced by an enzymatic hydrolysis process and followed by HPH were also characterized. Enzymatic hydrolysis is an environmentally friendly pre-treatment that does not generate toxic residues but induces the breakdown of cellulose polymer into smaller polymer branches, thus favoring the posterior mechanical treatment [69]. The fibrillation yield of the CMF-ENZ sample was low (4.9%) when compared with the nanocellulose obtained with the chemical pre-treatment [58,70].…”

Section: Discussionmentioning

confidence: 99%

Analysis of the In Vitro Toxicity of Nanocelluloses in Human Lung Cells as Compared to Multi-Walled Carbon Nanotubes

Pinto

Lourenço²,

Pedrosa

et al. 2022

Nanomaterials

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Cellulose micro/nanomaterials (CMNM), comprising cellulose microfibrils (CMF), nanofibrils (CNF), and nanocrystals (CNC), are being recognized as promising bio-nanomaterials due to their natural and renewable source, attractive properties, and potential for applications with industrial and economical value. Thus, it is crucial to investigate their potential toxicity before starting their production at a larger scale. The present study aimed at evaluating the cell internalization and in vitro cytotoxicity and genotoxicity of CMNM as compared to two multi-walled carbon nanotubes (MWCNT), NM-401 and NM-402, in A549 cells. The exposure to all studied NM, with the exception of CNC, resulted in evident cellular uptake, as analyzed by transmission electron microscopy. However, none of the CMNM induced cytotoxic effects, in contrast to the cytotoxicity observed for the MWCNT. Furthermore, no genotoxicity was observed for CNF, CNC, and NM-402 (cytokinesis-block micronucleus assay), while CMF and NM-401 were able to significantly raise micronucleus frequency. Only NM-402 was able to induce ROS formation, although it did not induce micronuclei. Thus, it is unlikely that the observed CMF and NM-401 genotoxicity is mediated by oxidative DNA damage. More studies targeting other genotoxicity endpoints and cellular and molecular events are underway to allow for a more comprehensive safety assessment of these nanocelluloses.

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Section: Discussionmentioning

confidence: 99%

Analysis of the In Vitro Toxicity of Nanocelluloses in Human Lung Cells as Compared to Multi-Walled Carbon Nanotubes

Pinto

Lourenço²,

Pedrosa

et al. 2022

Nanomaterials

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“…The lung tissue was found to be regulated and refined by the effect of anticancer agents, for that reason in the present work we investigated the morphological changes of compound 9d and 9e toward A549 cell line [29,30]. There are many reports concerned with morphological changes of other cell lines [31,32].…”

Section: Determination Of Morphological Changes Of A549cell Linementioning

confidence: 97%

Multicomponent reactions of cyanoacetanilide derivatives: Synthesis of coumarin and quinoline derivative and evaluations of their cytotoxicity

M. Mohareb,

K. Zahran,

R. Maghraby

et al. 2024

Bull. Chem. Soc. Eth.

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In this study, several fused heterocyclic systems involving tetrahydrobenzopyrans 7a-i and tetrahydrobenzopyridines 9a-i were synthesized. The reactions proceed via a multicomponent reaction of different aromatic aldehydes, cyanoacetanilide derivatives, and cyclohexane-1,3-dione. All the produced compounds were tested for their anticancer activity by using six cancer cell lines such as A549, HT-29, MKN-45, U87MG, SMMC-7721 and H460 utilizing foretinib as the positive control and the standard MTT assay in vitro. The results indicated that many synthesized derivatives were the most potent towards all the cancer cell lines used, six compounds 7e, 7f, 9b, 9d, 9e, and 9g showed the highest potency. The results obtained in this work enhance further investigations in the future. KEY WORDS: Multi-component reactions, Cyanoacetanilide, Pyran, Pyridine, Cytotoxicity Bull. Chem. Soc. Ethiop. 2024, 38(3), 739-750. DOI: https://dx.doi.org/10.4314/bcse.v38i3.15

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“…For that matter microenvironment of the lung tissue is regulated through complex and refined cell interactions. 44,45 For that reason we studied the effect of compound 13c (Fig. 2) and 13h (Fig.…”

Section: 5 Determination Of Morphological Changes Of A549 Cell Line B...mentioning

confidence: 99%

Cytotoxic Activities and Morphological Sudies of Thiophene, Thiazole and Pyridazine Derivatives Synthesized from Benzo[d]thiazole Derivatives

El-Sharkawy,

Mohareb,

Mukhtar

et al. 2023

ACSi

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The benzo[d]thiazole derivatives 4a–c were synthesized and used for the synthesis of thiophene derivatives 6a–f. They form the arylhydrazone derivatives 8a–i which were capable to form pyridazine derivatives 9a–i and 10a–i. The latter compounds reacted with thioglycolic acid to produce the thiazole derivatives 12a–i and 13a–i, respectively. The thiazole derivatives 15a–c were also produced from 4a–c through their reactions with elemental sulfur and phenylisothiocyanate. Further heterocyclization reactions of 15a were carried out to produce thiophene derivatives. Evaluations of the synthesized products were carried out against some selected cancer cell lines and the most active compounds were further evaluated against the seventeen cancer cell lines classified according to the disease. Morphological changes of A549 cell line by the effect of compounds 13c and 13h were studied using microenvironment of the lung tissue where an excellent result was obtained.

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Ultrastructural changes associated to the neuroendocrine transdifferentiation of the lung adenocarcinoma cell line A549

Cited by 10 publications

References 48 publications

Analysis of the In Vitro Toxicity of Nanocelluloses in Human Lung Cells as Compared to Multi-Walled Carbon Nanotubes

Analysis of the In Vitro Toxicity of Nanocelluloses in Human Lung Cells as Compared to Multi-Walled Carbon Nanotubes

Multicomponent reactions of cyanoacetanilide derivatives: Synthesis of coumarin and quinoline derivative and evaluations of their cytotoxicity

Cytotoxic Activities and Morphological Sudies of Thiophene, Thiazole and Pyridazine Derivatives Synthesized from Benzo[d]thiazole Derivatives

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