Ultrastructural changes in liver mast and plasma cells in pregnant rats with endotoxin shock

Nikulin, O. V.; Saakov, B. A.; Bardakhch'yan, E. A.

doi:10.1007/bf00838930

Cited by 2 publications

(2 citation statements)

References 2 publications

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“…1, b). Unlike the liver, where degranulated mast cells were detected during the initial period of ES [8], in the pancreas no total degranulation was observed in any case during the entire period of observation.…”

Section: Resultscontrasting

confidence: 68%

Ultrastructural changes in the pancreas and their role in the pathogenesis of myocardial injury in endotoxin shock

1998

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The pancreas in endotoxin shock is characterized by the hypoperfusion, prolonged cycle of acinar cell secretion, and activation of their lysosomal system. The rearrangements in the exocrine part of the gland involve the production of a myocardial depressant l~actor exerting a negative inotropic effect on the heart. Myocardial dysfunction is aggravated by myofibrillar contractures, myocytolysis, glycogenolysis, and circulatory disorders.

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Section: Resultscontrasting

confidence: 68%

Ultrastructural changes in the pancreas and their role in the pathogenesis of myocardial injury in endotoxin shock

1998

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“…Endotoxin stimulates connective tissue cells such as mast cells and may lead to the release of mediators and biologically active substances responsible for cardiac injury during endotoxemia. 32 This may be attributable to anaerobic metabolism.…”

Section: Discussionmentioning

confidence: 99%

Nitric Oxide Production and Energy State in the Heart after Endotoxin Administration

Nishigaki

Aramaki²,

Hirakawa³

et al. 1998

Jpn Circ J

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acterial lipopolysaccharide (endotoxin) induces the production of a large amount of nitric oxide (NO), which is a critical mediator for a variety of biologic functions. How NO contributes to heart failure is unclear.However, the excessive production of various endogenous mediators such as endothelium-derived relaxing factor (NO) after endotoxin administration might contribute to the depression of myocardial contractility and hypotension. 1,2 NO might have both physiologic and pathologic roles in heart failure. [3][4][5] The effects of endotoxin administration on NO production and cardiac dysfunction, especially cardiac energy status, have not been examined thoroughly. To elucidate the relationships among NO production, energy metabolism and morphologic changes, rat hearts were observed after endotoxin administration using magnetic resonance spectroscopy (MRS), biochemical, and electron microscopical techniques. Materials and MethodsAll studies were approved by the Animal Experimental Committee of Nippon Medical School. Thirty-two male Wistar rats (250-350 g) were injected ip with 10 mg/kg endotoxin, Escherichia coli lipopolysaccharide (Difco, Detroit, MI, USA). Thirteen control male rats were given ip injections of normal saline. ElectrocardiographyElectrocardiograms (ECGs) were recorded at 4, 6, and 8 h after endotoxin administration. For this procedure, rats were lightly restrained. Two thin leads were taped to the extremities, and bipolar recordings were obtained and compared with those of control animals without endotoxin administration. Plasma Nitrite MeasurementTwelve animals, including 3 control rats, were sacrificed at various time points (4, 6 and 8 h) after endotoxin administration and bled from their descending aorta. The blood was collected in heparinized tubes, which were centrifuged at 12,000 × g for 5 min in a microcentrifuge. The plasma was decanted into clean tubes and stored at -20°C. Before assay, the plasma was centrifuged (9,000 × g for 15 min at 4°C) with a Centricon-10 (exclusion molecular weight, 10000; Amicon, Danvers, MA, USA) and the filtrates obtained were used for the determination. The nitrite concentration was measured in 50-l samples of the plasma from 96-well plates incubated with an equal volume of Griess reagent (1% sulfanilamide, 0.1% N-1-naphthylenediamine dihydrochloride, and 2.5% phosphoric acid) at room temperature for 20 min in a microplate. The absorbance at 550 nm was measured and the nitrite concentration was determined using a curve calibrated from sodium nitrite standards. Electrophoresis and Western BlottingAfter endotoxin administration, 12 rat hearts were homogenized with lysis buffer [10 mmol/L Tris-HCl pH 7.4, 1 mmol/L NaCl, 5 mmol/L EDTA, 1% sodium dodecyl sulfate (SDS), 1 mmol/L phenylmethylsulfonyl fluoride Nitric Oxide Production and Energy State in the Heart after Endotoxin AdministrationJpn Circ J 1998; 62: 527 -531 Ryutaro Nishigaki, MD; Takumi Aramaki, MD; Keiko Hirakawa, Ph D*; Goro Asano, MD** To evaluate nitric oxide (NO) production and the energy ...

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Ultrastructural changes in liver mast and plasma cells in pregnant rats with endotoxin shock

Cited by 2 publications

References 2 publications

Ultrastructural changes in the pancreas and their role in the pathogenesis of myocardial injury in endotoxin shock

Ultrastructural changes in the pancreas and their role in the pathogenesis of myocardial injury in endotoxin shock

Nitric Oxide Production and Energy State in the Heart after Endotoxin Administration

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