Ultrastructural changes in murine liver following HSV infection and stroke

Motorna, Natalia; Рыбалко, С. Л.; Kvitnitskaya-Ryzhova, Tatyana; Starosyla, D. B.; Strokina, Iryna G; Kaminsky, R. F.; Savosko, S.I.; Sokurenko, L. M.; Chaikovsky, Yu. B.

doi:10.1515/cipms-2018-0002

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…It is currently widely recognized that any viral infection that damages endothelial cells of blood vessels can be reliably associated with the risk of vascular disease [7]. Biochemical analysis revealed that content of free and esterified cholesterol was higherin the aortic tissue specimens of CMV-infected animals with normocholesterolemia than in the models of non-infected animals.…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural Features of Herpesvirus-Induced Aortic Intima Damage in Mice Fed the High-Fat Diet

et al. 2020

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The aim: To study the effect of a high-fat diet (HFD) on the structural changes in the aortic intima in intact and HSV-1-infected mice using Scanning electron microscopy (SEM) and Transmission electron microscopy (TEM). Materials and methods: In experiments Balb/c mice were infected with the HVS-1 and fed high-fat diet and 12 weeks later aortic ultrastructure was examined by SEM and TEM methods. The animals were subdivided into four experimental groups: 1st group – HSV-1-infected animals; 2nd – animals consuming high-fat diet (HFD); 3rd – infected animals that were subsequently consuming a high-fat diet (HSV / HFD); 4th – animals consuming a high-fat diet that were subsequently infected with HSV-1 (HFD / HSV) (n = 6); and control group – intact animals. Results: HVS-1 impaired ultrastructural changes in aorta greater than high-fat diet and HVS-1 alone (higher density of lipid inclusions in the subendothelial space, necrosis of endothelial cells), and infection of mice after high-fat diet ended 100% mortality. The formation of atheroma in the aortic wall during HFD was not detected, but the initiative manifestations of atherogenesis have been identified and restricted in the aortic intima. These structural changes included lipid inclusions in the subendothelial space, cell damage and destruction, which lead to an increase cellular detritus in the 3rd (HSV / HFD) group. Conclusions: HSV infection potentiates the accumulation of lipid inclusions in the aortic intima during a HFD, facilitates infection and contributes to the development of acute infection.

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Section: Introductionmentioning

confidence: 99%

Ultrastructural Features of Herpesvirus-Induced Aortic Intima Damage in Mice Fed the High-Fat Diet

et al. 2020

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Structural changes in liver under conditions of experimental hemorrhagic stroke

Motorna

2019

Current Issues in Pharmacy and Medical Sciences

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The article describes the results of histological and morphometric studies of liver in Balb/c mice with hemorrhagic stroke. The hemorrhagic stroke was modeled in the animals by administering autoblood in volume of 0.1 ml in the right hemisphere, and within 5, 10 and 30 days an analysis of structural changes in the liver was performed. Progressive changes were established in terms of 5-10 days of the experiment. This consisted of changes in the sinusoidal capillaries and notable changes in the central veins of the liver lobuli. Herein, acute dilatation and erythrocytal stasis were most pronounced around the lumen of the central veins, while hepatocytes with signs of necrosis (severe cytoplasmic swelling, vacuolar dystrophic changes) were detected in the sinusoid capillaries. The results of the morphometry indicated an increase in the area of the nucleus and the cells caused by intracellular swelling, domination of euchromatin and decrease of total density of chromatin in nuclei. Partial regression of the diameter of sinusoidal capillaries and the area of hepatocytes were detected on the 30th day of the experiment. The changes in the sinusoidal capillaries of the liver lobules are assessed as secondary to stroke, as well as to changes in organ microcirculation, and are associated with dystrophic changes in the hepatocytes.

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Correction with thiocetam of lead nanoparticles influence on morpho-functional status of rat liver

Omelchuk

Aleksiichuk

Chaikovsky

et al. 2020

Current Issues in Pharmacy and Medical Sciences

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The aim of this paper is to investigate the influence of Thiocetam on morphological changes in the liver of rats and on biochemical changes in their blood after exposure to lead nanoparticles and compounds. The liver is an organ that performs a number of functions, such as the synthesis of enzymes, hormones, plasma components and the neutralization of toxins. It is involved in many metabolic processes in the body.In undertaking this, colloidal solutions of lead sulphide nanoparticles at dosages 10 nm and 30 nm were injected into two groups of rats, PbSnano1 and PbSnano2, respectively, while group Pb(NO3) received subcutaneously a solution of lead nitrate in ion form in a dose of 1.5 mg/kg (0.94 mg/kg lead, in lead equivalent). After 60 administrations (12 weeks) of the studied substances, the exposure was discontinued and the animals were observed for 18 weeks. Subsequently, half of each group received Thiocetam by injection (for 6 weeks at a dose of 250 mg/kg) while the other half did not. We then assessed the mean body weight, absolute and relative liver weight, blood biochemistry values (total protein, albumin, glucose, total lipids, cholesterol, triglycerides levels in blood serum) and morphological changes in hepatocytes (morphological slides, nuclei cross-sectional area and cytoplasm cross-sectional area).The outcome of this work showed that the mean body weight of animals exposed to nanoparticles with Tiocetam did not differ from that of animals exposed to nanoparticles without pharmacological correction, but relative liver weight was statistically significantly higher than the corresponding values in rats without pharmacological correction. The morphological picture in all study group animals was characterized by the normalization of microvessel blood filling, structure of hepatic plates, disappearance of infiltration with lymphocytes and histiocytes. No dystrophic changes in hepatocytes were found. All this indicates the feasibility of preventive measures during exposure to lead nanoparticles, by administering Thiocetam.In both series of animals exposed to lead nanoparticles (PbSnano1 and PbSnano2), the cross-sectional area of the hepatocytes cytoplasm and the cross-sectional area of the hepatocytes nuclei were smaller than just after exposure, but in the series with Thiocetam adminstration, all the values did not differ from those in the control.

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Ultrastructural changes in murine liver following HSV infection and stroke

Cited by 3 publications

References 13 publications

Ultrastructural Features of Herpesvirus-Induced Aortic Intima Damage in Mice Fed the High-Fat Diet

Ultrastructural Features of Herpesvirus-Induced Aortic Intima Damage in Mice Fed the High-Fat Diet

Structural changes in liver under conditions of experimental hemorrhagic stroke

Correction with thiocetam of lead nanoparticles influence on morpho-functional status of rat liver

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