Ultrastructural changes in porcine liver sinusoidal endothelial cells of machine perfused liver donated after cardiac death

Bochimoto, Hiroki; Ishihara, Yo; Iwata, Hiroyoshi; Kondoh, Daisuke; Obara, Hiromichi; Matsuno, Naoto

doi:10.3748/wjg.v28.i19.2100

Cited by 5 publications

(14 citation statements)

References 44 publications

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“…We also found that the MMP is favorable for the recovery of mitochondrial functional ultrastructure in liver sinusoidal endothelial cells (LSECs) rather than other temperature conditions of MP (Bochimoto et al, 2022). However, the MMP for 4 h after 60 min of warm ischemia using the University of Wisconsin gluconate (UW) solution induces irregular vacuolar structures in the cytoplasm of LSECs (Bochimoto et al, 2022). LSEC damage during MP appears to cause inferior outcomes following liver transplantation (Bhogal et al, 2018); thus a better condition of MMP that suppresses the dysfunction of LSEC should be established.…”

Section: Introductionmentioning

confidence: 82%

“…One of the critical concerns of livers after MMP is the irregular vacuolar structures in the LSECs (Bochimoto et al, 2022). It is well- known that autophagy is activated in the LSECs during machine perfusion to protect them from perfusion damages (Boteon et al, 2017), and thus these vacuolar structures appear to resemble autophagolysosome.…”

Section: Discussionmentioning

confidence: 99%

“…We previously showed that the midthermic MP (MMP) reduces the hepatocellular enzyme release (Matsuno et al, 2014;Obara et al, 2013) and that it relatively preserves the functional ultrastructure of hepatocytes (Bochimoto et al, 2017;Meng et al, 2016) and bile canaliculus (Ishihara et al, 2020) well in donor grafts. We also found that the MMP is favorable for the recovery of mitochondrial functional ultrastructure in liver sinusoidal endothelial cells (LSECs) rather than other temperature conditions of MP (Bochimoto et al, 2022). However, the MMP for 4 h after 60 min of warm ischemia using the University of Wisconsin gluconate (UW) solution induces irregular vacuolar structures in the cytoplasm of LSECs (Bochimoto et al, 2022).…”

Section: Introductionmentioning

confidence: 86%

“…The osmium maceration method was described previously (Bochimoto et al, 2017;Bochimoto et al, 2022;Ishihara et al, 2020). In brief, the liver tissues were biopsied, incised into small pieces, and fixed with a fixative mixture of 0.5% glutaraldehyde/0.5% paraformaldehyde in 0.1 MPB, for 30 min at 4 C, and then the tissue samples were post-fixed with 1% OsO 4 in 0.1 MPB for 6 h at 4 C. The samples were immersed into 25% dimethyl sulfoxide (DMSO) for 30 min and subsequently 50% DMSO for 30 min as cryoprotection.…”

Section: Osmium-maceration Scanning Electron Microscopymentioning

confidence: 99%

“…In this study, the effectiveness of oxygenation with HbV during the MMP was examined by the ultrastructural analysis in the donor liver grafts after the MMP for 4 h, and in those after the following ex vivo reperfusion for 2 h using diluted allogeneic blood (isolated reperfusion model; IRM) that mimics the inside of the body after transplantation (Kanazawa et al, 2020). In particular, we focused on two points for liver damage reported so far: the three-dimensional ultrastructure of the hepatocytes (Bochimoto et al, 2017;Ishihara et al, 2020) and the irregular vacuolar structures in the cytoplasm of LSECs (Bochimoto et al, 2022) by using osmium-maceration scanning electron microscopy (OM-SEM) and transmission electron microscopy (TEM). We used four perfusion conditions in the present study (Figure 1):…”

Section: Introductionmentioning

confidence: 99%

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Machine perfusion preservation with hemoglobin based oxygen vesicles alleviate ultrastructural damages in porcine liver donated after cardiac death

Zin,

Bochimoto,

Kondoh

et al. 2023

Microscopy Res & Technique

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Midthermic machine perfusion (MMP) of post‐circulatory arrest donor liver grafts has the advantage of preserving the functional ultrastructure of hepatocytes in donor grafts. It was reported that oxygenation during MMP reduces portal venous resistance and increases bile production. The MMP with hemoglobin‐based oxygen vesicles (HbV) keeps the lower aspartate aminotransferase level (an indicator of liver injury) and maintains the functional ultrastructure of mitochondria in the hepatocytes. To evaluated differences of ultrastructural damages in donor livers between the MMP with and without HbV, porcine liver grafts after 60 min of warm ischemia were perfused at 22°C for 4 h with or without HbV, and a part of liver grafts were analyzed by transmission electron microscopy (TEM) and osmium‐maceration scanning electron microscopy (OM‐SEM). The remaining grafts were perfused with autologous blood at 38°C for 2 h in an isolated liver reperfusion model (IRM) that mimics the inside of the body after transplantation, and then analyzed by TEM and OM‐SEM. Hepatocytes after MMP had small round mitochondria with rod‐shaped cristae and reticulovesicular rough endoplasmic reticulum (rER) in both HbV(+) and HbV(−) livers. After IRM of HbV(+) livers, the well‐developed lamellar rER was often found in hepatocytes. Liver sinusoidal endothelial cells (LSECs) after MMP contained some large vacuolar structures containing amorphous garbage in the cytoplasm, and their size along with appearance frequency were smaller and lower, respectively, in HbV(+) livers than HbV(−). Oxygenation during the MMP by using HbV suppressed the ultrastructural damages in donor livers, in particular for the LSECs.Research Highlights Liver sinusoidal endothelial cells after midthermic machine perfusion had large vacuolar organelles with amorphous garbage. Oxygenation during the perfusion made them less and smaller, ultrastructurally supporting its utility.

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Section: Introductionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 86%

Section: Osmium-maceration Scanning Electron Microscopymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Machine perfusion preservation with hemoglobin based oxygen vesicles alleviate ultrastructural damages in porcine liver donated after cardiac death

Zin,

Bochimoto,

Kondoh

et al. 2023

Microscopy Res & Technique

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The role of liver sinusoidal endothelial cells in liver remodeling after injury

Li¹,

Wang²

2023

Hepatobiliary & Pancreatic Diseases International

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Hepatic Ischemia-reperfusion Injury: Protective Approaches and Treatment

Singh,

Gupta,

Kumar

et al. 2023

CMP

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Ischemia and reperfusion damage to the liver is one of the major causes of hepatic dysfunction and liver failure after a liver transplant. The start of hepatic ischemia-reperfusion damage is linked to metabolic acidosis, Kupffer cells, neutrophils, excessive calcium, and changes in the permeability of the mitochondrial membrane. Hypoxia activates Kupffer cells, resulting in the production of reactive oxygen species (ROS). These ROS when accumulated, causes apoptosis and necrosis, as well as activate immune and inflammatory responses that involve many cells and signalling molecules. Numerous antioxidant compounds have been researched to lessen oxidative stress and thus serve as potential compounds to deal the ischemia-reperfusion damage. This article confers a deep understanding of the protective effects of some effective therapies, including hepatoprotective agents, attenuation of an increase in xanthine oxidase activity, and administration of antioxidants like N-acetylcysteine, superoxide dismutase (SOD), and ornithine.

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Ultrastructural changes in porcine liver sinusoidal endothelial cells of machine perfused liver donated after cardiac death

Cited by 5 publications

References 44 publications

Machine perfusion preservation with hemoglobin based oxygen vesicles alleviate ultrastructural damages in porcine liver donated after cardiac death

Machine perfusion preservation with hemoglobin based oxygen vesicles alleviate ultrastructural damages in porcine liver donated after cardiac death

The role of liver sinusoidal endothelial cells in liver remodeling after injury

Hepatic Ischemia-reperfusion Injury: Protective Approaches and Treatment

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