Ultrastructural characteristics on capillary of giant panda

Abstract

Electron microscopic observation on ultrastructural characteristics of the capillary ofgiant panda was presented. The samples were taken from mesentery and another samples were taken from mucosa of intestine. They were fixed in 2.5% phosphatebuffered glutaraldehyde and 1% osmic acid, and dehydrated by graded series of acetone. Then they were embedded with Epon 812. For orientation under phasecontrast microscope, 0.5--1.0 u thick sections were prepared from the epoxyembedded material. After that, ultrathin sections (30-50) were taken with ultramicrotome LKB-V type and stained by uranyl acetate and lead citrate and examined under DXB2-12 Electron Microscope.Electron microscopic observation showed the capillary diameter in the mesentery microcirculation is larger and capillary wall is thin. The nucleus in endothelium was large and wasn't regular in the shape. Chromatin was loose in the nucleus. There was little plasma inthe endothelium and it looked loose. Organelles in the cytoplasm were little and vacuoles were more in endothelium. The distribution of plasma in the endothelium was different with endothelium of human body.