Ultrastructural characterization of cells in primary cultures from different adult tissues of Biomphalaria tenagophila TAIM, a strain that is absolutely resistant to Schistosoma mansoni infection

Silva-Neto, Aristeu; Silva, Luciana Maria; Alves, Luíz Carlos; Brayner, Fábio André; Fortes-Dias, Consuelo Latorre; Coelho, Paulo Marcos Zech

doi:10.1016/j.micron.2014.05.011

Cited by 2 publications

(4 citation statements)

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“…In the present study, a wide diversity of cells was observed in the DG-derived cultures ( Figure 4A). The majority of cells in culture displayed cytoplasmic granulations (Figure 4A-E), which is consistent with the expected profile for secretory gland tissues 12 . In Figure 4A, it is possible to identify the presence of cells with apparent morphological resemblance to Type I or Type II cell populations from the APO cultures 10 .…”

Section: Discussionsupporting

confidence: 85%

“…The culture medium was replaced with Schneider's insect medium and the cultures were maintained at 26°C. The condition ensured at least 90% cell viability after 36 h in culture 12 .…”

Section: Discussionmentioning

confidence: 99%

“…Successful primary cultures of the amebocyteproducing region (APO) were shown to be capable of eliminating S. mansoni sporocysts in vitro 10,11 . Cells in primary cultures from the saccular portion of the kidney (SK) of BtT were indistinguishable from those obtained in APO-derived cultures 12 . Based on the cell resemblance and anatomical proximity of APO and kidney inside the mantle cavity of Biomphalaria, we suggested that primary cultures of kidney-derived cells from BtT might participate in hematopoiesis and in host response to parasitic invasion 12 .…”

Section: Introductionmentioning

confidence: 96%

“…Cells in primary cultures from the saccular portion of the kidney (SK) of BtT were indistinguishable from those obtained in APO-derived cultures 12 . Based on the cell resemblance and anatomical proximity of APO and kidney inside the mantle cavity of Biomphalaria, we suggested that primary cultures of kidney-derived cells from BtT might participate in hematopoiesis and in host response to parasitic invasion 12 . In the present study, the hypothesis was tested by challenging cultured cells derived from the SK of BtT with S. mansoni sporocysts.…”

Section: Introductionmentioning

confidence: 96%

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Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni sporocysts

Silva-Neto

Fonseca

Silva

et al. 2020

Rev. Soc. Bras. Med. Trop.

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Introduction: Biomphalaria snails may display varying levels of susceptibility to Schistosoma mansoni infection. We have been developing an in vitro model to study the interaction between the snail and the parasite, using tissue-derived cell cultures from Biomphalaria. Methods: The digestive gland-and kidney-derived cells from primary cultures of resistant (B. tenagophila Taim) and susceptible (B. tenagophila HM and B. glabrata BH) strains of Biomphalaria were exposed to S. mansoni sporocysts. Results: S. mansoni sporocysts were surrounded and encapsulated exclusively by cells derived from the digestive gland (DG) of B. tenagophila Taim. The process was followed by a marked decrease in the number of free sporocysts in the culture medium. The morphological characteristics of DG-derived cells in culture have been described. Conclusions: Cells derived from DG (but not SK) primary cultures of B. tenagophila Taim may participate in S. mansoni sporocyst control.

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Section: Discussionsupporting

confidence: 85%

“…The culture medium was replaced with Schneider's insect medium and the cultures were maintained at 26°C. The condition ensured at least 90% cell viability after 36 h in culture 12 .…”

Section: Discussionmentioning

confidence: 99%