Ultrastructural characterization of normal Merkel cells in the dog

Ramı́rez, G.A.; Rodrı́guez, Francisco; Herráez, P.; Castro-Alonso, Ayoze; Andrada, M.; Espinosa-de-los-Monteros, Antonio

doi:10.1111/vde.12230

Cited by 7 publications

(17 citation statements)

References 41 publications

(86 reference statements)

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“…The granular staining also showed differences in intensity between MCs from the same or different locations in the same animal or between different individuals at the present study. Previously, similar differences in staining of MCs were also reported to occur under IHC procedures and electron microscopy (Beiras et al, ; Ramirez et al, ; Ramirez et al, ). These differences are related with the presence of several neuropeptides in the cytoplasmic granules of the mammalian MCs and with the existence of diverse mammalian MCs subpopulations with a variety of secretory elements (Gauweiler et al, ; Tachibana and Nawa, ; Ramirez et al, ).…”

Section: Discussionsupporting

confidence: 68%

Study on the Role of Histochemical Stains in Identifying Merkel Cells in Dogs

Ramı́rez

Monteros

2018

The Anatomical Record

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Merkel cells (MCs) are neuroendocrine cells involved with tactile sense, growth, differentiation, and homeostasis of the skin as well as in different cutaneous diseases. Specific staining techniques are required for their identification because they are not easily visible in paraffin sections stained with hematoxylin and eosin. The present study assess the histochemical features of the MCs in dogs comparing with those described for other mammals in the literature and with the use of immunohistochemistry. A systematic study of samples from MCs-rich areas from healthy dogs was carried out by use of several histologic stains, including metachromatic staining, silver stains, methylene blue, periodic acid-Schiff stain, and osmium-based staining method. MCs were detected by the Grimelius argyrophilic stain in 86.7% of the specimens. The staining was showed as dark-brown granular cytoplasmic and consistently polarized to the basal cell cytoplasm matching with the cellular distribution of the characteristic neurosecretory granules. Some modifications in the standard staining protocol, including rinsing, silver reimpregnation, and counterstain dye, enhanced the MCs identification in stratified squamous epithelium. When compared with Cytokeratin 20-immunolabeled serial sections several MCs appeared nonstained with the argyrophilic method. These differences in MC numbers between stains were statistically significant. Other histologic stains failed to identify MCs in the specimens. The results of this study indicate that Grimelius argyrophilic stain is a suitable method for demonstration of MCs in the stratified squamous epithelium of skin and mucosa. Discussion on its utility when compared with immunohistochemistry and a review of the scientific literature is also presented.

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Section: Discussionsupporting

confidence: 68%

Study on the Role of Histochemical Stains in Identifying Merkel Cells in Dogs

Ramı́rez

Monteros

2018

The Anatomical Record

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“…In addition, being the structural cytoskeleton, CKs in the MCs seem to be participating in the cell plasticity necessary for mechanoreceptive function (Eispert et al 2009). In the NSE-positive cells, the immunoreaction was dispersed throughout the cytoplasm in correspondence to the distribution of ribosomes and the rough endoplasmic reticulum observed in the MCs under electron microscopy (Gu et al 1981;Vinores et al 1984;Ramírez et al 2015). It has been noted that the number of ribosomes per cell varies with the cell cycle (Darzynkiewicz et al 1979); therefore NSE expression by MCs may be different depending on the cell status.…”

Section: Discussionmentioning

confidence: 83%

“…Cytokeratins are integral constituents of the cytoskeleton in the mammalian MCs (Eispert et al 2009;Halata et al 2010). Immunoreactive products in the MCs labelled with anti-cytokeratin antibodies appear to be homogeneously distributed within the cytoplasm, matching with the typical arrangement of cytokeratins in the form of fine intermediate filaments distributed within the cell (Halata et al 2010;Ramírez et al 2015). In addition, being the structural cytoskeleton, CKs in the MCs seem to be participating in the cell plasticity necessary for mechanoreceptive function (Eispert et al 2009).…”

Section: Discussionmentioning

confidence: 98%

Study of Merkel cells in the dog through the immunohistochemical expression of five different commercial antibodies: comparative analysis

Ramı́rez

Rodrı́guez

Suárez‐Bonnet

et al. 2017

Journal of Applied Animal Research

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Merkel cells (MCs) are cutaneous specialized cells with a wide immunohistochemical profile, including low molecular weight cytokeratins and various endocrine and neural markers. Differences in expression of these markers may be a consequence of contradictory results in studies of MCs in normal and damaged canine tissues. The present study aimed to compare five different commercial available antibodies developed against cytokeratins 8 + 18 and 20, neuron-specific enolase, chromogranin A, and synaptophysin on formalin-fixed paraffin-embedded samples of hard palate, cheek skin including sinus hair follicles or vibrissae, nasal planum, and footpads. The antibodies showed great variability with respect to quality and intensity of immunoreactivity to identify MCs. Anti-Cytokeratin 20 antibody is more effective to recognize MCs in the dog skin followed by the antibodies against neuron-specific enolase, cytokeratins 8 and18, chromogranin A, and synaptophysin. There was also a significant difference in intensity of immunoreaction scores between them depending on the location examined. These results represent a necessary basic background for future studies of the role of these cells in normal and damaged canine tissues.

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“…Mammalian skin has time‐honored sensory organs: Vater‐Pacini corpuscles (Pacini, 1835; Vater, 1741), Meissner corpuscles (Wagner & Meissner, 1852), Krause endings (Krause, 1860), Merkel cell‐neurite complex (Merkel, 1875; Ramirez et al, 2015), Ruffini corpuscles (Ruffini, 1893), and Golgi‐Mazzoni corpuscles (Ruffini, 1893). All gathered under the term sensory nerve formations (Malinovsky, 1996).…”

Section: Introductionmentioning

confidence: 99%

A new type of somatosensory organ in the nasolabial skin of the dog

Elofsson

Kröger

2020

Journal of Morphology

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A new morphological type of somatosensory organ is described. It is found in the glabrous skin of the dog nose (rhinarium or planum nasale) and situated in dermis papillae. The otherwise thick epidermis forms a thin window above the organ. There are only a few layers of keratinocytes in the window and the corneocytes are much thinner than elsewhere. The organ consists of highly branching cells that wrap naked nerve endings emanating from myelinated nerve fibers originating in the outer dermal nerve plexus. The structure entirely fills the top of the dermal papilla. The intercellular spaces of the organ and its surroundings are occupied by an extended areolar basal lamina.

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Ultrastructural characterization of normal Merkel cells in the dog

Cited by 7 publications

References 41 publications

Study on the Role of Histochemical Stains in Identifying Merkel Cells in Dogs

Study on the Role of Histochemical Stains in Identifying Merkel Cells in Dogs

Study of Merkel cells in the dog through the immunohistochemical expression of five different commercial antibodies: comparative analysis

A new type of somatosensory organ in the nasolabial skin of the dog

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