Ultrastructural cytochemical identification of peroxisomes as organelles of terminal oxidation in amitochondrial trichomonad flagellates

Cornford, Marcia Albright

doi:10.31274/rtd-180817-1024

Cited by 2 publications

(3 citation statements)

References 89 publications

(147 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Amoebida (Entamoeba species), Diplomonadida, Trichomonadida, Hypermas tigina, Holotricha, and Oligotricha of the rumen of Bovidae and of the coecum of Equidae. There are, however, microbody-like structures present in several of these groups that were assumed, on the basis of positive histochemical reactions for dehydrogenases and with DAB,to represent redox organelles in trichomonads (28,98,104) and hypermastiginids (59). Biochemical data are available only for tri chomonads.…”

Section: Anaerobic Protozoamentioning

confidence: 99%

See 1 more Smart Citation

BIOCHEMISTRY OF PROTOZOAN MICROBODIES: Peroxisomes, α-Glycerophosphate Oxidase Bodies, Hydrogenosomes

Müller¹

1975

Annu. Rev. Microbiol.

View full text Add to dashboard Cite

Section: Anaerobic Protozoamentioning

confidence: 99%

“…65). There structures are osmiophilic (41) and give positive DAB reaction (28,65). Fractions enriched in microbodies were separated from Monocercomonas sp.…”

Section: Trichomonadidamentioning

confidence: 99%

BIOCHEMISTRY OF PROTOZOAN MICROBODIES: Peroxisomes, α-Glycerophosphate Oxidase Bodies, Hydrogenosomes

Müller¹

1975

Annu. Rev. Microbiol.

View full text Add to dashboard Cite

“…Various suggestions have been made with respect to the possible relationships of the trichomonad microbody-like particles to either mitochondria or peroxisomes (2,13,14,16,20,21,24) . So far, these assumptions have been tested with histochemical techniques only (13,14,35,36), and the present study is the first, to our knowledge, to allow a more rigorous discussion of the question in terms of presence and absence of enzymes, at least in T. foetus.…”

Section: Localization Of Oxidoreductasesmentioning

confidence: 99%

Biochemical Cytology of Trichomonad Flagellates

Müller

1973

The Journal of Cell Biology

103

View full text Add to dashboard Cite

To determine the localization of several enzymes in Tritrichomonas foetus, the axenic KV-1 strain was grown in Diamond's medium with bovine serum, homogenized in 0 .25 M sucrose, and subjected to analytical differential and isopycnic centrifugation . The fractions were assayed for their enzymatic composition and examined electron microscopically . NADH and NADPH dehydrogenases, about 90% of the catalase, and two hydrolases, agalactosidase and manganese-activated ß-galactosidase I are in the nonsedimentable part of the cytoplasm . a-Glycerophosphate and malate dehydrogenases are associated with a large particle, whose equilibrium density in sucrose gradients is 1 .24 . This particle corresponds to that population of the paracostal and paraxostylar granules which, having a uniform granular matrix surrounded by a single membrane, resemble microbodies from other organisms . The small sedimentable portion of catalase (about 10% of the total activity) is not associated with these granules and equilibrates at density 1 .22 . The nature of the subcellular entity carrying catalase could not be ascertained . Hydrolases with a pH optimum around 6-6 .5 (protease, ß-N-acetylglucosaminidase, ß-N-acetylgalactosaminidase, and cation-independent ß-galactosidase II), as well as a large part of acid phosphatase, are associated with a population of large particles which equilibrate at densities from 1 .15 to 1 .20 . The hydrolases in these granules lose their structure-bound latency easily after freezing and thawing . These particles correspond to another population of the paracostal and paraxostylar granules which have varied shape and inhomogeneous content with frequent myelin figures, indicating a digestive function . The rest of the phosphatase and most of the acid ß-glucuronidase activity are in a smaller granule fraction with an equilibrium density around 1 .18 . The latency of these enzymes is quite resistant to freezing and thawing . This particle population consists of smaller, very often flattened vesicles and granules, many of which are clearly fragments of the prominent Golgi apparatus of the cell .

show abstract

Ultrastructural cytochemical identification of peroxisomes as organelles of terminal oxidation in amitochondrial trichomonad flagellates

Cited by 2 publications

References 89 publications

BIOCHEMISTRY OF PROTOZOAN MICROBODIES: Peroxisomes, α-Glycerophosphate Oxidase Bodies, Hydrogenosomes

BIOCHEMISTRY OF PROTOZOAN MICROBODIES: Peroxisomes, α-Glycerophosphate Oxidase Bodies, Hydrogenosomes

Biochemical Cytology of Trichomonad Flagellates

Contact Info

Product

Resources

About