Ultrastructural Cytochemical Localization of Polysaccharides in Dental Plaque

Barkin, Michael E.

doi:10.1177/00220345700490054301

Cited by 10 publications

(4 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cells were predominantly coccoid in shape with a majority exhibiting cell wall features compatible with those of Gram-positive micro organisms. These cells frequently contained cytoplasmic inclusions resembling carbohydrate granules [24][25][26] (Fig. 5).…”

Section: Normal Samplesmentioning

confidence: 99%

See 1 more Smart Citation

Structure of the Microbial Flora Associated with Periodontal Health and Disease in Man: A Light and Electron Microscopic Study

Listgarten

1976

Journal of Periodontology

498

304

View full text Add to dashboard Cite

Teeth slated for extraction were evaluated with respect to their periodontal status and classified accordingly into five categories; namely normal, gingivitis, periodontitis, periodontosis and postperiodontosis. After processing, one approximal surface of each tooth was sampled at various levels in an apico-occlusal direction for light and electron microscopic study of the associated bacterial flora. In normal samples, the flora consisted of a relatively thin, adherent bacterial layer confined to the enamel surface. The cells were predominantly coccoid in shape with cell wall features compatible with those of Gram-positive organisms. Isolated filamentous or branching forms and some Gram-negative bacteria were noted on the surface of the more apical portion of the bacterial layer. No flagellated cells or spirochetes were present. Gingivitis samples yielded a relatively more voluminous and complex supragingival flora with relatively more filamentous bacteria and more cells with a Gram-negative cell wall ultrastructure. These samples also contained corncob formations on the surface of supragingival deposits, and flagellated cells with spirochetes within the predominantly Gram-negative flora of the sulcus bottom. Supragingival bacterial deposits of periodontitis samples were similar to those observed in gingivitis. The subgingival flora consisted of relatively fewer cells adherent to the root surface with a concomitant increase in the population of Gram-negative and flagellated cells, as well as spirochetes. The tissue side of the subgingival flora generally exhibited a distinctive concentration of "test-tube brush" formations, spirochetes of predominantly medium size, and assorted cell types peculiar to this region. A transitional flora generally separated the supra- from the subgingival microbial population. Periodontosis samples had a relatively sparse, predominantly Gram-negative flora. A unique electron-dense, lobulated cuticular deposit covered the majority of the samples studied. Postperiodontosis samples were much more similar in their microbial flora to the periodontitis group. The results suggest that (1) a certain microbial flora may be compatible with a state of periodontal health; (2) a different flora is associated with varying degrees of periodontal disease; (3) the structure and composition of the supragingival flora differs markedly from that of the subgingival flora; (4) with the exception of periodontis, the alterations of the microbial flora as periodontal disease increases inseverity parallel the changes described previously in the microbial population collected on artificial crowns during experimentally induced gingivitis. The use of the expressions "microbial flora" or "microbial population" is considered preferable to the terms "plaque", "materia alba", or "debris" in reference to the microbiota of the gingival sulcus region.

show abstract

Section: Normal Samplesmentioning

confidence: 99%

“…Within this superficial layer of bacteria, "bristle brush" or "test-tube brush" formations were commonly observed (Figs. [23][24][25]. These consisted of a central axis composed of one or several large filamentous bacteria, approximately 1 µm in diameter, oriented perpendicu larly to the surface of the bacterial mass.…”

Section: Periodontitis Samplesmentioning

confidence: 99%

Structure of the Microbial Flora Associated with Periodontal Health and Disease in Man: A Light and Electron Microscopic Study

Listgarten

1976

Journal of Periodontology

498

304

View full text Add to dashboard Cite

show abstract

“…Dental plaque is considered to be an important etiological factor in the pathogenesis of caries and periodontal disease (7,8,27). A number of investigators have studied the morphology of dental plaque at the ultrastructural level (1,5,6,24,25,29) and have shown that it is composed of a wide variety of microorganisms. Although it is sometimes possible to distinguish gram-positive from gram-negative bacteria in the electron microscope by the structure of their cell wall (4, 9, 23), it is generally not possible to identify specific species by their morphology alone.…”

mentioning

confidence: 99%

Immunoelectron microscopic identification and localization of Streptococcus sanguis with peroxidase-labeled antibody: localization of Streptococcus sanguis in intact dental plaque

Lai¹,

Listgarten²,

Rosan³

1975

Infect Immun

View full text Add to dashboard Cite

Streptococcus sanguis has been localized ultrastructurally within intact dental plaque by means of an indirect technique which utilizes horseradish peroxidase-labeled antibody. The technique allows for complete diffusion of the reagents to all portions of the plaque specimens. Control procedures can be carried out on serial sections of plaque with a bacterial composition similar to that of the experimental specimen. The 30-mum-thick sections can be examined in the light microscope to localize areas specifically labeled with peroxidase prior to cutting ultra-thin sections for electron microscopy. This study demonstrated that specific bacteria can be localized within intact dental plaque. The results also indicated that S. sanguis grows in dental plaque as columnar shaped microcolonies perpendicular to the tooth surfaces. Growth appears to be by cell division rather than deposition of new cells at the surfaces. Despite their relatively good structural preservation, the cells in the deeper (older) layers of plaque appear to have lost some of their antigenic activity in comparison to the cells near the surface.

show abstract

“…[8][9][10][11][12][13] The electron density of the capsular polysaccharide of many bacteria is very low when stained with conventional methods. For example, osmium and lead do not make this cell component visible in the electron micrographs.…”

mentioning

confidence: 99%

Basic Biological Sciences Electron Microscopic Visualization of Extracellular Polysaccharides on the Cell Wall of Some Streptococci

Lounatmaa¹,

Meurman

1980

J Dent Res

View full text Add to dashboard Cite

show abstract

Ultrastructural Cytochemical Localization of Polysaccharides in Dental Plaque

Cited by 10 publications

References 17 publications

Structure of the Microbial Flora Associated with Periodontal Health and Disease in Man: A Light and Electron Microscopic Study

Structure of the Microbial Flora Associated with Periodontal Health and Disease in Man: A Light and Electron Microscopic Study

Immunoelectron microscopic identification and localization of Streptococcus sanguis with peroxidase-labeled antibody: localization of Streptococcus sanguis in intact dental plaque

Basic Biological Sciences Electron Microscopic Visualization of Extracellular Polysaccharides on the Cell Wall of Some Streptococci

Contact Info

Product

Resources

About