Ultrastructural cytochemistry of oxytalan fibers in the periodontal ligament and microfibrils in the aorta with the periodic acid‐thiocarbohydrazide‐silver proteinate method

Takagi, Minoru; Parmley, Richard T.; Yagasaki, Hiroshi; Toda, Yoshihisa

doi:10.1111/j.1600-0714.1984.tb01469.x

Cited by 15 publications

(9 citation statements)

References 24 publications

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“…Microfibrils in the adventitia were TA-UA negative, elastase-resistant, PA-TCH-SP positive, and Con A reactive, and these cytochemical properties are consistent with those in the lowest vertebrate, lamprey (Isokawa et al, 1989), as well as in mammals (Takagi et al, 1984;Baba et al, 1985;Fanning and Cleary, 1985). In the intima and media, microfibrils were ambiguous in routine osmicated morphological preparations, but their presence was revealed in unosmicated specimens or after elastase digestion, so the microfibrils in these tunics are presumably embedded entirely in osmiophilic substance, or elastin.…”

Section: Discussionsupporting

confidence: 66%

Ultrastructural and cytochemical study of elastic fibers in the ventral aorta of a teleost, Anguilla japonica

1990

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Previous studies have revealed that amorphous elastin and microfibrils are structural entities of mammalian elastic fibers. Elastin shows a wide phylogenetic distribution, but the presence of elastin-associated microfibrils has not been demonstrated in teleost aorta. Thus, we have ultrastructurally and cytochemically examined elastic fibers in the ventral aorta of eel, a teleost, by utilizing routine uranyl acetate and lead double staining, the tannic acid (pH 7.0)-uranyl acetate (TA-UA) method, elastase en bloc digestion, Thiéry's periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) method, and the horseradish-peroxidase-labeled concanavalin A (Con A) method. In the ventral aorta of eel, a little ultrastructural difference between elastic fibers in the intima and media and those in the adventitia was noticed, but in either tunic each elastic fiber was basically composed of a "fibrillar core" and surrounding microfibrils. The fibrillar core was a collection of fibrils which showed a tendency to coalesce with each other, and these constituent fibrils were TA-UA positive and elastase-sensitive, representing their nature of elastin. By contrast, microfibrils associated with the fibrillar core were TA-UA negative and elastase-resistant, and their glycoproteinaceous nature was demonstrated by PA-TCH-SP and Con A methods. Thus, this study provides evidence for the presence of elastin-associated microfibrils in teleost aorta. These results are discussed in relation to the topographical difference of elastic fibers in eel aortic wall.

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Section: Discussionsupporting

confidence: 66%

Ultrastructural and cytochemical study of elastic fibers in the ventral aorta of a teleost, Anguilla japonica

1990

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“…11). Both fibrillin glycoprotein and carbohydrate moieties of the in situ fibrillin microfibrils are known to be detectable with reagents such as Schiff and silver proteinate after periodate oxidation (Takagi et al, 1984;Sakai et al, 1986;Isokawa et al, 1989Isokawa et al, , 1990. These histochemical characteristics common to reticular fibers may account for argyrophilia conferred possibly by the microfibrillar bundles.…”

Section: Discussionmentioning

confidence: 99%

Subectodermal microfibrillar bundles are organized into a distinct parallel array in the developing chick limb bud

et al. 2004

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In this study, a unique fiber system in the subectodermal mesenchyme of the chick limb bud was visualized immunohistochemically with the use of a novel monoclonal antibody termed "FB1." This antibody stained a subset of extracellular fibers in the embryonic mesenchyme. Among the fibers visualized, those running perpendicularly to the limb bud ectoderm became progressively prominent in their thickness and length, and organized into a parallel array in the subectodermal region. This fiber system was distinct from that of major collagens, fibronectin, or tenascin. A molecule immunoprecipitated with FB1 comigrated with JB3 antigen, or chicken fibrillin-2. The fibers visualized immunohistochemically by FB1 and JB3 were indistinguishable from each other, and ultrastructurally appeared to be bundles composed of tubular-like microfibrils that originated directly from the ectodermal basal lamina. They lacked the amorphous deposits that are characteristic of elastin. A similar array of subectodermal fibers was also found in the developing axilla and some truncal regions, again well before the development of a definitive dermis. These findings suggest that a parallel array of subectodermal FB1-positive fibers constitutes a precocious fiber system in the presumptive dermis prior to the substantial formation of collagenous fibers. These fibers could be developmentally linked to oxytalan fibers, which are known to be present in the papillary dermis in mature cutaneous tissue.

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“…1981 ]. pineal gland [Calvo and Boya, 1983], aortic adventitia [Takagi et al. 1984] and meninx [Carbonell et al.…”

Section: Discussionmentioning

confidence: 99%

Myofibroblasts in Human Palatal Mucosa

Boya

Carbonell²,

Martinez³

1988

Cells Tissues Organs

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Myofíbroblasts in human osseous palatal mucosa are described. They appear as fusiform or ramified cells, rich in homogeneous 60- to 70-Ångström-thick micro filaments, rough endoplasmic reticulum cisternae, and abundant pinocytotic vesicles in relation with the plasma membrane. On the surface of these cells there are small areas covered by basal lamina. Contacts between myofibroblast processes and other tissue elements are described. Small clusters of oxytalan fibers appear in the vicinity of these cells.

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Ultrastructural cytochemistry of oxytalan fibers in the periodontal ligament and microfibrils in the aorta with the periodic acid‐thiocarbohydrazide‐silver proteinate method

Cited by 15 publications

References 24 publications

Ultrastructural and cytochemical study of elastic fibers in the ventral aorta of a teleost, Anguilla japonica

Ultrastructural and cytochemical study of elastic fibers in the ventral aorta of a teleost, Anguilla japonica

Subectodermal microfibrillar bundles are organized into a distinct parallel array in the developing chick limb bud

Myofibroblasts in Human Palatal Mucosa

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