Ultrastructural Demonstration of the Negative Surface Charge on Human Trophoblast

Kawagoe, Koh; Kawana, Takashi; Sakamoto, Shoichi

doi:10.1267/ahc.13.254

Cited by 12 publications

(4 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The surface glycoprotein of the normal human trophoblast, demonstrated with ruthenium red staining, has been shown to consist of two different layers, a thinner and thicker one, which corresponded to the distribution of negative surface charge on the cell surface (3,14). The most striking finding, shown in the present study, is that the surface glycoprotein distributed on the trophoblastic plasma membrane forming regularly situated aggregates, 15 to 20 mµ in diameter separated by 50 to 100 mµ from neighbouring ones.…”

Section: Discussionsupporting

confidence: 62%

“…Currie and Bagashawe (8) presented the attractive hypothesis that the peritrophoblastic surface coat of sialomucin bears a high electronegative charge and escapes attacks from maternal lymphocyte by electrochemical repulsion. In these aspects, the basic structure and the character of the negative surface charge of the normal human trophoblast have been studied with the discussion on its biological role (13,14).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Negative surface charge of abnormal human trophoblast.

Kawagoe

Kawana

Sakamoto

1981

Acta Histochem. Cytochem

Self Cite

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 62%

mentioning

confidence: 99%

Negative surface charge of abnormal human trophoblast.

Kawagoe

Kawana

Sakamoto

1981

Acta Histochem. Cytochem

Self Cite

View full text Add to dashboard Cite

“…The local effect of HCG, which enables tissue of fetal origin to survive against maternal immunologic attacks, has been reported in several papers (2,9,10,12,18). The characteristic localization of HCG at the nidatory site, that is, with a great amount of HCG, existed at the proximal part of the anchoring villi.…”

Section: Discussionmentioning

confidence: 94%

Localization of human chorionic gonadotropin at the nidatory site of tubal pregnancy.

Kawagoe

Kawana

Sakamoto

1981

Acta Histochem. Cytochem

Self Cite

View full text Add to dashboard Cite

Localizationof human chorionic gonadotropin (HCG) at the nidatroy sites of tubal pregnancies was studied using immunoperoxidase and immunofluorescent methods.In the villous trophoblast including anchoring villi, HCG was exclusively located in the syncytiotrophoblast.The amount of HCG which syncytiotrophoblast contained decreased in relation to the degenerative changes of the placental villi. In the anchoring villi, HCG was shown to exist more at the proximal part of the syncytiotrophoblast than at the distal part of the same villi. A markedly strong immunoreaction was observed at the syncytiotrophoblast which was scattered in the trophoblastic shell and also partly covered the trophoblastic shell. Not only the mononuclear trophoblast with abundunt glycogen in the cytoplasm, which usually existed in the trophoblastic column and shell and was classified as transitional trophoblast based on ultrastructural features, but also the mononuclear trophoblast that existed in the fibrinoid layer showed a weak but not negligible immune reaction.The characteristic localization of HCG at the nidatory site of the tubal pregnancy seemed to imply some special roles of HCG on nidation and various phenomena correlated with it. And it was also shown from these findings that the transitional trophoblast contained a small amount of HCG and that HCG concentration of the villous syncytiotrophoblast reflected the degenerative changes of the villi.In studying the nidatory site of early human pregnancy, extrauterine pregnancy provides many valuable materials which are not easily obtainable from the normal intrauterine pregnancy. Recent studies concerning the biological role of HCG has revealed its effect in transplantation immunity (2, 10) is rather local than systemic (l, 6, 19). The nidatory site of the human pregnancy, which nature gives us as an experiment of allogeneic transplantation, provides us precious information to clarify the role of HCG further. Using these materials, HCG localization at the nidatory site was studied immunohistochemically. The results thus obtained were compared with the basic structure of the nidatory site and fine structures of each trophoblast, which were examined with the electron microscope in one case. Special references were made on the relationship between the localization of HCG and its well-known effects on local immunity. The amount of HCG was examined in comparison with the degenerative changes of the trophoblast.

show abstract

“…A variety of histochemical and ultrastructural studies have localized anionic glycocon-jugates in placental tissue. Some of the negatively charged sites on the cell surface can be removed with neuraminidase (King, 1981;Kawagoe et al, 1980), indicating the presence of sialic acid. Martin et al (1974) demonstrated significant reduction in dialyzed iron surface staining after neuraminidase and testicular hyaluronidase digestion indicating the presence of a surface GAG in addition to sialic acid.…”

mentioning

confidence: 99%

Ultrastructural localization of glycosaminoglycans in human term placenta

1984

View full text Add to dashboard Cite

Sulfated glycoconjugates were stained in normal human term placentas using Spicer's high-iron diamine (HID) method with thiocarbohydrazide and silver proteinate (TCH-SP) enhancement. Specific identification of glycosaminoglycans (GAG) was accomplished by digestion of the stained material with chondroitinase ABC or AC for removal of chondroitin sulfates and nitrous acid for removal of N-sulfated GAGs. The syncytiotrophoblast apical surface demonstrated moderate to intense staining with HID-TCH-SP, which was removed by prior digestion with the chondroitinases, but not by nitrous acid. The syncytiotrophoblast basal surface and endothelial cell surfaces lacked sulfate staining. A few cytoplasmic granules in syncytiotrophoblast cells demonstrated staining similar to the apical surface. Three layers of the basal lamina were identified in these preparations. The lamina lucida immediately beneath the syncytiotrophoblast and the majority of the lamina densa stained weakly or not at all, whereas the underlying lamina diffusa and stroma demonstrated moderate to intense staining. The majority of lamina diffusa staining was removed by chondroitinase ABC or AC; the remaining material was removed by nitrous acid digestion. Thus the syncytiotrophoblast surface contains a chondroitin sulfate and the basal lamina contains a mixture of intensely stained chondroitin sulfate and a weakly stained N-sulfated GAG.

show abstract

Ultrastructural Demonstration of the Negative Surface Charge on Human Trophoblast

Cited by 12 publications

References 42 publications

Negative surface charge of abnormal human trophoblast.

Negative surface charge of abnormal human trophoblast.

Localization of human chorionic gonadotropin at the nidatory site of tubal pregnancy.

Ultrastructural localization of glycosaminoglycans in human term placenta

Contact Info

Product

Resources

About