Ultrastructural Evaluation of the Human Oocyte at the Germinal Vesicle Stage during the Application of Assisted Reproductive Technologies

Palmerini, Maria Grazia; Antonouli, Sevastiani; Macchiarelli, Guido; Cecconi, Sandra; Bianchi, Serena; Khalili, Mohammad Ali; Nottola, Stefania Annarita

doi:10.3390/cells11101636

Cited by 5 publications

(3 citation statements)

References 103 publications

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“…In humans, and likely other species, fertility may be altered by mutations in mitochondrial DNA in association with ovarian aging, possibly due to ROS production [ 3 ]. Interestingly, the replacement of damaged mitochondria by healthier counterparts in human oocytes may soon improve assisted reproductive technologies (ART) [ 4 ].…”

Section: Early Developmentmentioning

confidence: 99%

Molecular and Clinical Advances in Understanding Early Embryo Development

Winkle

2023

Cells

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Section: Early Developmentmentioning

confidence: 99%

Molecular and Clinical Advances in Understanding Early Embryo Development

Winkle

2023

Cells

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“…The process of oocyte development includes two-stage meiotic division, resulting in the formation of haploid female gametes ready for fertilization and further zygote development [1]. The diplotene stage of meiotic prophase-at which the oocyte nucleus is called the germinal vesicle (GV) [2]-is crucial for the oocyte to transit from growth to maturation. Oocyte maturation is a complex process in which cytoplasmic and nuclear factors contribute [3].…”

Section: Introductionmentioning

confidence: 99%

Chromatin Morphology in Human Germinal Vesicle Oocytes and Their Competence to Mature in Stimulated Cycles

Salimov,

Lisovskaya,

Otsuki

et al. 2023

Cells

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The search for simple morphological predictors of oocyte quality is an important task for assisted reproduction technologies (ARTs). One such predictor may be the morphology of the oocyte nucleus, called the germinal vesicle (GV), including the level of chromatin aggregation around the atypical nucleolus (ANu)—a peculiar nuclear organelle, formerly referred to as the nucleolus-like body. A prospective cohort study allowed distinguishing three classes of GV oocytes among 135 oocytes retrieved from 64 patients: with a non-surrounded ANu and rare chromatin blocks in the nucleoplasm (Class A), with a complete peri-ANu heterochromatic rim assembling all chromatin (Class C), and intermediate variants (Class B). Comparison of the chromatin state and the ability of oocytes to complete meiosis allowed us to conclude that Class B and C oocytes are more capable of resuming meiosis in vitro and completing the first meiotic division, while Class A oocytes can resume maturation but often stop their development either at metaphase I (MI arrest) or before the onset of GV breakdown (GVBD arrest). In addition, oocytes with a low chromatin condensation demonstrated a high level of aneuploidy during the resumption of meiosis. Considering that the degree of chromatin condensation/compaction can be determined in vivo under a light microscope, this characteristic of the GV can be considered a promising criterion for selecting the best-quality GV oocytes in IVM rescue programs.

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“…The proportion of immature oocytes becomes more relevant in patients with advanced age, poor ovarian response (POR), or polycystic ovary syndrome (PCOS) by exceeding 50% of the retrieved oocytes [ 7 ]. A GV stage oocyte is characterized by a large nucleus (germinal vesicle) in prophase I of the first meiotic division and a prominent spherical nucleolus [ 8 ]. An MI oocyte forms following germinal vesicle breakdown and is identified by the absence of a visible GV or first polar body [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Rescue IVM of Denuded GV- and MI-Stage Oocytes of Premenopausal Rats with Oncostatin M, Insulin-like Growth Factor I, and Growth Hormone

Akdemir

Çakıl

Selam

et al. 2022

Life

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Immature oocytes are retrieved and matured through in vitro maturation (IVM). Maturation, fertilization rates, and embryo development via IVM are all lower than those found in vitro fertilization (IVF) cycles. We investigated the effects of oncostatin M (OSM), insulin-like growth factor-1 (IGF-I), and growth hormone (GH) in rescue IVM. A total of 111 germinal vesicle (GV) and 17 metaphase I (MI) oocytes were obtained after conventional IVF from 28 female Wistar albino rats. Denuded immature oocytes were cultured in maturation medium supplemented with OSM, IGF-1, or GH. The quantities of metaphase II (MII) oocytes matured from the GV stage were 17 of 30 (56.6%), 15 of 28 (53.5%), 10 of 30 (33.3%), and 7 of 23 (30.3%), in control, OSM, IGF-I, and GH groups, respectively. Maturation rates in control and OSM groups were higher than those in IGF-I and GH groups (p = 0.001). The quantities of MII oocytes matured from MI stage were 7 of 7 (100%), 4 of 4 (100%), 1 of 1 (100%), and 1 of 5 (20%) in control, OSM, IGF-I, and GH groups, respectively. Maturation rates from MI to MII stages in control, OSM, and IGF-I groups were higher than those in the GH group (p = 0.004). Acceptable maturation rates are observed with OSM in rat oocytes in rescue IVM.

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Ultrastructural Evaluation of the Human Oocyte at the Germinal Vesicle Stage during the Application of Assisted Reproductive Technologies

Cited by 5 publications

References 103 publications

Molecular and Clinical Advances in Understanding Early Embryo Development

Molecular and Clinical Advances in Understanding Early Embryo Development

Chromatin Morphology in Human Germinal Vesicle Oocytes and Their Competence to Mature in Stimulated Cycles

Rescue IVM of Denuded GV- and MI-Stage Oocytes of Premenopausal Rats with Oncostatin M, Insulin-like Growth Factor I, and Growth Hormone

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