Ultrastructural evidence of stromal/epithelial interactions in the human endometrial cycle

Roberts, Daniel K.; Walker, N; Lavia, Lynn A.

doi:10.1016/0002-9378(88)90084-1

Cited by 51 publications

(20 citation statements)

References 13 publications

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“…Recent reports from our laboratory and others (3)(4)(5)(6) (25)(26)(27)(28)(29)(30). While a principal role for the stroma has been often proposed in mediating steroid action during growth and differentiation in numerous adult tissues (23), including the endometrium (24), our study demonstrates that stromal cells can mediate progesterone suppression of an epithelial-specific protein.…”

Section: Discussionmentioning

confidence: 48%

“…A principal role for cell-cell interactions has long been recognized in mediating aspects of steroid action during growth and differentiation in numerous adult tissues, including the endometrium (23,24). The cellular language by which cell-cell interactions can occur is complex, involving growth factors (25)(26)(27), cytokines (28), extracellular matrix components (29), and direct cell contact (30).…”

mentioning

confidence: 99%

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Stromal-epithelial interaction mediates steroidal regulation of metalloproteinase expression in human endometrium.

Osteen

Rodgers

Gaire

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

175

144

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The hallmark of the menstrual cycle is extensive steroid-dependent tissue turnover. Estrogen mediates endometrial cell growth and structural remodeling, whereas progeserone suppresses estrogen-dependent proliferation and promotes cellular differentiation. In nonfertile cycles, tissue degradation and menstruation occur as a consequence of steroidal deprivation as the ovarian corpus luteum fails. Stromalepithelial interactions are recognized as a necesary component in mediating steroid-induced endometrial turnover. Specific mRNAs for metalloproteinases of the stromelysin family are expressed during endometrial growth and menstrual breakdown but are absent in the progestin-dominated secretory phase. This expression pattern suggss involvement of stromelysins in remodeling the extracellular matrix of the endometrium during tissue growth and breakdown and implicates progesteronE in the suppression of these enzymes. We examined the regulation of endometrial stromelysins in explant cultures and found no acute effect ofestradiol on their expression, whereas progesterone was a potent inhibitor of stromelysin expression. Progesterone also suppressed stromelysin expression in cultures ofisolated stromal cells, but epithelial cells were progesterone insensitive. Coculture ofrecombined stromal and epithelial cells restored steroidal suppression of the epithelial-specific metalloproteinase. Our data confirm that progesterone inhibits endometrial stromelysins and further demonstrate the necessity for a stromalderived factor(s) as a mediator of steroid suppression of an epithelial metalloproteinase.The human endometrium undergoes extensive estradiolinduced growth and remodeling during the proliferative phase of the menstrual cycle, followed by secretory maturation in response to postovulatory progesterone (1). This rapid and extensive degree of steroid-mediated tissue development, which rivals that of many neoplasias, appears to be a necessary component of providing an environment suitable for sustaining hemochorial placentation (2). In the absence of implantation and the continued progestational environment of pregnancy, the superficial functionalis region of the endometrium undergoes degradation and is expelled with menstrual blood flow. Several laboratories have recently described the expression of matrix metalloproteinases (MMPs) in the normal, cycling human endometrium (3-6). These enzymes degrade many components of the extracellular matrix, including proteoglycans, glycoproteins, and basement membrane collagens (7). Our studies (5, 6) identified a cell type-specific expression pattern of mRNAs coding for members of the stromelysin family only during the proliferative and premenstrual/menstrual stage of the cycle; none of the enzymes were identified during the progesterone-dominated secretory menstrual interval. This pattern of expression suggests an active role for stromelysins during growth-associated structural remodeling as well as during the extensive tissue breakdown associated with menstruation.MMPs of the st...

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Section: Discussionmentioning

confidence: 48%

mentioning

confidence: 99%

Stromal-epithelial interaction mediates steroidal regulation of metalloproteinase expression in human endometrium.

Osteen

Rodgers

Gaire

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

175

144

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“…23 Histological studies have shown that endometriotic, as well as endometrial, epithelium seems to be dependent on stroma cells for survival. 24 When the stroma cells are replaced with fibrotic tissue during the healing process, the related epithelium will undergo atrophy and often shed. This might be a factor contributing to the common finding of small, isolated atrophic glands without surrounding stroma in ectopic sites.…”

Section: Discussionmentioning

confidence: 99%

Quantitative enzyme immunoassay and semiquantitative immunohistochemistry of oestrogen and progesterone receptors in endometriotic tissue and endometrium.

Bergqvist¹,

Fernö²,

Skoog³

1997

Journal of Clinical Pathology

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“…Expression of Cxs may be affected not only by chemical factors but also by interactions between stromal and epithelial cells (Roberts et al, 1988) and by alterations in metabolism related to the microvasculature. Ultrastructural studies have been performed on gap junctions in the human endometrial epithelium (Roberts et al, 1988) and in the human endometrial stroma (Parmley et al, 1990). Roberts et al (1988) reported an increase in the number and size of gap junctions between glandular epithelia during the transition from the early proliferative phase to the early secretory phase.…”

Section: Discussionmentioning

confidence: 99%

“…Ultrastructural studies have been performed on gap junctions in the human endometrial epithelium (Roberts et al, 1988) and in the human endometrial stroma (Parmley et al, 1990). Roberts et al (1988) reported an increase in the number and size of gap junctions between glandular epithelia during the transition from the early proliferative phase to the early secretory phase. Their data are similar to ours, showing that expression of Cx26 and Cx32 proteins increases markedly in the early secretory phase and diminishes in the late secretory phase, though a peak of expression was seen in the mid-secretory phase in our study.…”

Section: Discussionmentioning

confidence: 99%

Co-ordinated expression of connexins 26 and 32 in human endometrial glandular epithelium during the reproductive cycle and the influence of hormone replacement therapy

et al. 1997

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Ultrastructural evidence of stromal/epithelial interactions in the human endometrial cycle

Cited by 51 publications

References 13 publications

Stromal-epithelial interaction mediates steroidal regulation of metalloproteinase expression in human endometrium.

Stromal-epithelial interaction mediates steroidal regulation of metalloproteinase expression in human endometrium.

Quantitative enzyme immunoassay and semiquantitative immunohistochemistry of oestrogen and progesterone receptors in endometriotic tissue and endometrium.

Co-ordinated expression of connexins 26 and 32 in human endometrial glandular epithelium during the reproductive cycle and the influence of hormone replacement therapy

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