Ultrastructural Features of Myenteric Ganglia of Adult Wistar Rats (<i>Rattus norvegicus</i>)

Natali, Maria Raquel Marçal; Neto, Marcílio Hubner Miranda; Orsi, Antônio Marcos

doi:10.1046/j.1439-0264.2000.00293.x

Cited by 11 publications

(12 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The inexistence of morphological changes in the plexus of rats during aging process has also been reported in previous studies (Natali et al 2000, Porto et al 2012.…”

Section: Discussionsupporting

confidence: 73%

Benefits of caloric restriction in the myenteric neuronal plasticity in aging rats

Pereira

Mari

Stabille

et al. 2014

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

Aging is a biologic process characterized by progressive damage of structures and functions of organic systems. In gastrointestinal tract, it can involve enteric nervous system, which plays an important role in digestion and absorption of nutrients, causing hastening of intestinal transit thus reducing its absorptive function. Caloric restriction has been used in several studies with the intention of delaying deleterious effects of aging. This study aimed to evaluate the effects of caloric restriction on myenteric neurons of ileum by aging in rats. 30 Wistar rats were grouped as follows: GI (animals aged 6 months fed with normal diet), GII (animals aged 18 months fed with normal diet) and GIII (animals aged 18 months subject to 31% of caloric restriction). The rats of the GI group were euthanized at 6 months of age and after experimental period of 12 months animals of the group GII and GIII were euthanized, the ileum of all groups were collected, measured and processed by NADPH-dp and Acetylcholinesterase. Quantitative analysis of neurons revealed that aging promotes the increasing of myenteric neurons NADPH-dp and reduces Acetylcholinesterase neuronal population. However, in the cellular profile area, were not observed significant differences between the groups. The caloric restriction has been efficient and can be used preventively because it minimizes quantitative changes associated with aging on ileum myenteric plexuses.

show abstract

“…The inexistence of morphological changes in the plexus of rats during aging process has also been reported in previous studies (Natali et al 2000, Porto et al 2012.…”

Section: Discussionsupporting

confidence: 73%

Benefits of caloric restriction in the myenteric neuronal plasticity in aging rats

Pereira

Mari

Stabille

et al. 2014

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

show abstract

“…Hypoplasia and hypotrophy of the mucosa in malnourished rats 6,7 and the increase of the myenteric neuronal density while reducing the protein level on the chow to 8% 6,[8][9][10][11][12][13] . As there is a gap in the literature with respect to the effects of severe malnutrition (4%) on the enteric neurons, this paper analyzed the neuronal density of the neurons from the myenteric plexus in the ileum of malnourished rats subjected to a hypoproteic diet (4%) for 90 days.…”

mentioning

confidence: 99%

Quantitative analysis of the neurons from the myenteric plexus in the ileum of rats submitted to severe protein deficiency

Moreira

Hermes

Almeida

et al. 2008

Arq. Neuro-Psiquiatr.

View full text Add to dashboard Cite

-The effects of protein malnutrition on the quantitative aspects of the myenteric plexus in the ileum of adult Rattus norvegicus were assessed. Thirty 90-day-old rats were divided into two groups: Control Group (CG, n=15) and Experimental Group (EG, n=15). The CG received 26% protein chow and the EG received 4% protein chow for 90 days. At the end of the experiment, the animals from the CG weighed 369.63±26.33, and the ones from the EG 215.34±56.31. The ileum was submitted to Giemsa, NADH-and NADPH-diaphorase technique in order to evidence nervous cells in the whole-mount preparations. Animals from the EG presented a 41.75% body weight loss in relation to the CG as well as 17.6% length reduction for the ileum-jejunum. Moreover, the organ was 41% lighter for the EG. Giemsa-stained neurons were 17.02% more concentrated in the EG (p>0.05). NADH-diaphorase-stained neurons were 26.6% more concentrated in the EG (p<0.05), while the NADPH-diaphorase were 26.28% more concentrated in this group (p<0.05).KEy worDs: protein malnutrition, rat ileum, enteric neurons. Análise quantitativa dos neurônios do plexo mientérico do íleo de ratos submetidos a intensa carência de proteínasResumo -Avaliou-se o efeito da desnutrição protéica sobre o número de neurônios mientéricos do íleo de ratos adultos. Foram utilizados 30 animais (90 dias de idade), divididos em dois grupos: controle (GC, n=15) e experimental (GE, n=15), sendo oferecido ao GC ração com teor protéico de 26% e, para o GE, ração com 4% de proteína, durante 90 dias. os animais do grupo controle pesaram 369,63±26,33g e o experimental 215,34±56,31g. Preparados de membrana do íleo foram submetidos à técnica de Giemsa, NADH-e NADPH-diaforase. os animais do GE apresentaram perda de peso de 41,75%, em relação ao GC e redução do comprimento do jejuno-íleo de 17,6%, além disso, o órgão apresentou-se 41% mais leve no GE. os neurônios corados com a técnica de Giemsa apresentaram-se 17,02% mais concentrados no GE (p>0,05). os neurônios NADH-diaforase apresentaram-se 26,60% mais concentrados no GE (p<0,05). E os neurônios NADPH-diaforase apresentaramse 26,28% mais concentrados neste grupo (p<0,05).PAlAvrAs-CHAvE: má nutrição protéica, íleo, rato, neurônio entérico.Paper presented as part of the first author´s graduation monograph to the UNIPAr Nursing Departament; Experimental Neurogastroenterology laboratory -UNIPAr; Umuarama, Pr Brazil. Financial support: UNIPAr.

show abstract

“…Os gânglios mioentéricos foram evidenciados entre as camadas circular e longitudinal da túnica muscular, sendo esta localização bem descrita em mamíferos (GABELA, 1972;1989;NATALI et al, 2000;FURNESS, 2009).…”

Section: Microscopia Eletrônica De Transmissãounclassified

“…Importante ressaltar que a disposição e a função protetora deste envoltório de tecido conjuntivo se assemelham com o sistema nervoso central (STERNINI, 1988;NATALI, 2000).…”

Section: Microscopia Eletrônica De Transmissãounclassified

“…Não visualizamos alterações nas células mioentéricas e gliais com o envelhecimento e RC. As características morfológicas dos neurônios e células da glia entérica, por nós observadas, foram descritas no intestino grosso de cobaias, macacos e humanos (BAUMGARTEN et al, 1970;NATALI et al, 2000) e no intestino delgado de ratos (MARI et al, 2008). Natali et al (2000) relatam que, independente da espécie animal ou do segmento intestinal, os núcleos dos neurônios mioentéricos possuem características ultraestruturais peculiares que permitem distingui-los das células gliais entéricas.…”

Section: Microscopia Eletrônica De Transmissãounclassified

See 1 more Smart Citation

Efeitos da alimentação com diferentes níveis calóricos nos neurônios mioentéricos do cólon de ratos Wistar durante o processo de envelhecimento

Mari¹

View full text Add to dashboard Cite

Efeitos da alimentação com diferentes níveis calóricos nos neurônios mioentéricos do cólon de ratos wistar durante o processo de envelhecimentoThe last years were characterized by a significant increase in the elderly population.For this reason, concern for the elderly is becoming a constant in our society, since the aging results in impairment of bodily function, or not accompanied by structural changes. In the gastrointestinal tract (GI), the aging process can cause significant morphological changes. The regulation of motility of the gut is controlled mainly by myenteric neurons of the enteric nervous system. The decrease in the density of neurons in the colon can lead to a reduction in the frequency and amplitude of contraction expressed as colonic constipation. The actions aimed at reducing the effects of aging on the GI system include those related to diet, among which stands out caloric restriction. Caloric restriction, and have a protective effect on the myenteric neurons during aging, is also responsible for the decrease of neuronal death by apoptosis, a process accentuated with aging. Thus, this study was to evaluate the effects of different levels of calorie restriction on the plasticity of myenteric neurons NADPH and acetylcholinesterase positive of the colon of rats during the aging process by means of ultrastructural (transmission electron microscopy) and morphoquantitative analysis. Therefore, we used 40 male Wistar rats (Rattus norvegicus), divided into four groups (n = 10): CI-animals six months; SR-animals 18 month fed a normal diet; RCI -animals 18 months fed a diet with 12% caloric restriction; RCII-animals 18 months fed a diet with 30% caloric restriction. At six months, the animals were transferred to the vivarium sector, where they remained up to 18 months under controlled conditions of temperature and light and water ad libitum. It was observed that the RC level of 30% effectively minimized the deleterious effects of aging on myenteric neurons, which may be adopted as an alternative against the common gastrointestinal disorders in the elderly.

show abstract

Ultrastructural Features of Myenteric Ganglia of Adult Wistar Rats (Rattus norvegicus)

Cited by 11 publications

References 12 publications

Benefits of caloric restriction in the myenteric neuronal plasticity in aging rats

Benefits of caloric restriction in the myenteric neuronal plasticity in aging rats

Quantitative analysis of the neurons from the myenteric plexus in the ileum of rats submitted to severe protein deficiency

Efeitos da alimentação com diferentes níveis calóricos nos neurônios mioentéricos do cólon de ratos Wistar durante o processo de envelhecimento

Contact Info

Product

Resources

About