Ultrastructural immunocytochemical localization of μ-opioid receptors in dendritic targets of dopaminergic terminals in the rat caudate–putamen nucleus

Wang, H; Moriwaki, Akiyoshi; Wang, J.B; Uhl, George R.; Pickel, Virginia M.

doi:10.1016/s0306-4522(97)00253-4

Cited by 45 publications

(43 citation statements)

References 67 publications

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“…Our findings are consistent with electrophysiologic studies in the NTS slice preparation, suggesting that MOR ligands can act at postsynaptic sites at which they have been shown to inhibit neurons through potassium channels (Rhim et al, 1993). The extrasynaptic distribution of MOR in dendrites is consistent with that shown previously in the NTS (Cheng et al, 1996a) and in other brain regions (Wang et al, 1997;Wang and Pickel, 1998) and supports a modulatory role for MOR in the postsynaptic responses to excitatory vagal afferents. Extrasynaptic receptors for peptides, such as the opioids, are consistent with the release of the peptide from dense core vesicles in axon terminals, and these vesicles are not associated preferentially with synaptic contacts (Pierce et al, 1999).…”

Section: Mor Distribution In Vagal Target Dendritessupporting

confidence: 90%

?-opioid receptors are present in vagal afferents and their dendritic targets in the medial nucleus tractus solitarius

et al. 2000

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Section: Mor Distribution In Vagal Target Dendritessupporting

confidence: 90%

?-opioid receptors are present in vagal afferents and their dendritic targets in the medial nucleus tractus solitarius

et al. 2000

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“…Immuno-electron microscopy revealed that MOR in the rat CPu was predominantly distributed along non-synaptic portions of the dendritic plasma membranes of medium spiny neurons [29], which, based on our results, would be lipid rafts-enriched portions. Indeed, Sheng and colleagues showed that lipid rafts exist abundantly in dendrites of cultured hippocampal neurons [30].…”

Section: Thalamus: An Exception Regarding Opioid Receptors-lipid Raftsupporting

confidence: 72%

Brain region-specific N-glycosylation and lipid rafts association of the rat mu opioid receptor

Huang

Chen

et al. 2008

Biochemical and Biophysical Research Communications

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The mu opioid receptor (MOR) in the rat and mouse caudate putamen (CPu) and thalamus was demonstrated as diffuse and broad bands by western blot with polyclonal antibodies against a Cterminal peptide of MOR, which were absent in the cerebellum and brains of MOR-knockout mice. The electrophoretic mobility of MOR differed in the two brain regions with median relative molecular masses (Mr's) of 75 kDa (CPu) vs. 66 kDa (thalamus) for the rat, and 74 kDa (CPu) vs. 63 kDa (thalamus) for the mouse, which was due to its differential N-glycosylation. Rat MOR in CPu was found mainly associated with low-density cholesterol-and ganglioside M1 (GM1)-enriched fractions (lipid rafts), while the MOR in the thalamus was present in rafts and non-rafts without preference. Cholesterol reduction by methyl-β-cyclodextrin decreased DAGMO-induced [ 35 S]GTPγS binding in rat CPu membranes to a greater extent than in the thalamus membranes.

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“…This type of terminal is typical of cholinergic interneurons (Izzo and Bolam, 1988;Calabresi et al, 2000), as well as dopaminergic nigrostriatal neurons (Bouyer et al, 1984;Freund et al, 1984;Wang et al, 1997). The presence of DOR in acetylcholine-and/or dopamine-containing terminals is consistent with (1) light microscopic studies showing DOR localization within cholinergic interneurons in the CPN (Mansour et al, 1994) and (2) electron microscopic evidence that DOR and the dopamine transporter are colocalized in axon terminals in the ventral striatum (Svingos et al, 1999).…”

Section: Preferential Presynaptic Distribution Of Dorsupporting

confidence: 71%

Preferential Cytoplasmic Localization of δ-Opioid Receptors in Rat Striatal Patches: Comparison with Plasmalemmal μ-Opioid Receptors

Wang

Pickel

2001

J. Neurosci.

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The activation of ␦-opioid receptors (DORs) in the caudateputamen nucleus (CPN) produces regionally distinct changes in motor functions, many of which are also influenced by opioids active at -opioid receptors (MORs). These actions most likely occur in MOR-enriched patch compartments in the CPN. To determine the functional sites for DOR activation and potential interactions involving MOR in these regions, immunoperoxidase and immunogold-silver labeling methods were applied reversibly for the ultrastructural localization of DOR and MOR in single rat brain sections containing patches of the CPN. DOR immunoreactivity was commonly seen within the cytoplasm of spiny and aspiny neurons, many of which also expressed MOR. In dendrites and spines, DOR labeling was preferentially localized to membranes of the smooth endoplasmic reticulum and spine apparatus, whereas MOR showed a prominent plasmalemmal distribution. DOR-and/or MOR-labeled spines received asymmetric, excitatory synapses, some of which showed notable perforations, suggesting the involvement of these receptors in activity-dependent synaptic plasticity. DORs were more frequently detected than were MORs within axon terminals that formed either asymmetric synapses with spine heads or symmetric synapses with spine necks. Our results suggest that in striatal patches, DORs, often in cooperation with MORs, play a direct modulatory role in controlling the postsynaptic excitability of spines, whereas presynaptic neurotransmitter release onto spines is mainly influenced by DOR activation. In comparison with MOR, the prevalent association of DOR with cytoplasmic organelles that are involved in intracellular trafficking of cell surface proteins suggests major differences in availability of these receptors to extracellular opioids.

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Ultrastructural immunocytochemical localization of μ-opioid receptors in dendritic targets of dopaminergic terminals in the rat caudate–putamen nucleus

Cited by 45 publications

References 67 publications

?-opioid receptors are present in vagal afferents and their dendritic targets in the medial nucleus tractus solitarius

?-opioid receptors are present in vagal afferents and their dendritic targets in the medial nucleus tractus solitarius

Brain region-specific N-glycosylation and lipid rafts association of the rat mu opioid receptor

Preferential Cytoplasmic Localization of δ-Opioid Receptors in Rat Striatal Patches: Comparison with Plasmalemmal μ-Opioid Receptors

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