Ultrastructural Localization of Acid Phosphatase in Germ Cells of Chick Embryo Left Ovary

Civinini, Annalena; Mastrolia, Lucia

doi:10.1159/000163349

Cited by 2 publications

(2 citation statements)

References 7 publications

(9 reference statements)

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“…In vivo histology and in vitro experiments conducted over thirty years ago indicated that female germ cells enter meiotic prophase during embryogenesis in birds, as occurs in mammals [ 24 - 26 ]. These early studies variably reported that meiosis in the left female chicken gonad commences on day 14, 15, 16, 17 or 18 of development [ 14 , 15 , 27 ]. We have found that chicken STRA8 is up-regulated specifically in the left female gonad from day 12.5, as assessed by qRT-PCR, and meiosis is detectable by SCP3 immunostaining in some cells at day 15.5, becoming widespread at day 16.5 and 17.5 (Fig.…”

Section: Discussionmentioning

confidence: 99%

Onset of meiosis in the chicken embryo; evidence of a role for retinoic acid

et al. 2008

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Background: Meiosis in higher vertebrates shows a dramatic sexual dimorphism: germ cells enter meiosis and arrest at prophase I during embryogenesis in females, whereas in males they enter mitotic arrest during embryogenesis and enter meiosis only after birth. Here we report the molecular analysis of meiosis onset in the chicken model and provide evidence for conserved regulation by retinoic acid.

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Section: Discussionmentioning

confidence: 99%

Onset of meiosis in the chicken embryo; evidence of a role for retinoic acid

et al. 2008

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“…In the ZW left gonad, somatic and germ cells proliferate in the cortex, which thickens considerably, while the cords of the medulla become vacuolated, forming lacunae. The germ cells of the left ZW gonad enter in meiosis asynchronously from day 15 [5], [6], [7]. The meiotic entry of female germ cells is then completed before hatching.…”

Section: Introductionmentioning

confidence: 99%

Gene Expression Profiling Reveals New Potential Players of Gonad Differentiation in the Chicken Embryo

et al. 2011

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BackgroundIn birds as in mammals, a genetic switch determines whether the undifferentiated gonad develops into an ovary or a testis. However, understanding of the molecular pathway(s) involved in gonad differentiation is still incomplete.Methodology/Principal FindingsWith the aim of improving characterization of the molecular pathway(s) involved in gonad differentiation in the chicken embryo, we developed a large scale real time reverse transcription polymerase chain reaction approach on 110 selected genes for evaluation of their expression profiles during chicken gonad differentiation between days 5.5 and 19 of incubation. Hierarchical clustering analysis of the resulting datasets discriminated gene clusters expressed preferentially in the ovary or the testis, and/or at early or later periods of embryonic gonad development. Fitting a linear model and testing the comparisons of interest allowed the identification of new potential actors of gonad differentiation, such as Z-linked ADAMTS12, LOC427192 (corresponding to NIM1 protein) and CFC1, that are upregulated in the developing testis, and BMP3 and Z-linked ADAMTSL1, that are preferentially expressed in the developing ovary. Interestingly, the expression patterns of several members of the transforming growth factor β family were sexually dimorphic, with inhibin subunits upregulated in the testis, and bone morphogenetic protein subfamily members including BMP2, BMP3, BMP4 and BMP7, upregulated in the ovary. This study also highlighted several genes displaying asymmetric expression profiles such as GREM1 and BMP3 that are potentially involved in different aspects of gonad left-right asymmetry.Conclusion/SignificanceThis study supports the overall conservation of vertebrate sex differentiation pathways but also reveals some particular feature of gene expression patterns during gonad development in the chicken. In particular, our study revealed new candidate genes which may be potential actors of chicken gonad differentiation and provides evidence of the preferential expression of BMPs in the developing ovary and Inhibin/Activin subunits in the developing testis.

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Ultrastructural Localization of Acid Phosphatase in Germ Cells of Chick Embryo Left Ovary

Cited by 2 publications

References 7 publications

Onset of meiosis in the chicken embryo; evidence of a role for retinoic acid

Onset of meiosis in the chicken embryo; evidence of a role for retinoic acid

Gene Expression Profiling Reveals New Potential Players of Gonad Differentiation in the Chicken Embryo

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