Ultrastructural localization of catalase on ultracryotomic sections of mouse liver by ferritin-conjugated antibody technique

Yokota, Sadaki; Nagata, T.

doi:10.1007/bf00495964

Cited by 19 publications

(6 citation statements)

References 18 publications

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“…1E). In contrast, catalase, which in mouse liver is found in peroxisomal and nonperoxisomal fractions such as Golgi and endoplasmic reticulum (41), was enriched by a factor of 2.4. After normalization to protein concentration and volume of resuspension, it was calculated that 2% of total hepatic FATP2 resides in PMP70-containing peroxisomes.…”

Section: Resultsmentioning

confidence: 90%

FATP2 is a hepatic fatty acid transporter and peroxisomal very long-chain acyl-CoA synthetase

Falcon

Doege

Fluitt

et al. 2010

American Journal of Physiology-Endocrinology and Metabolism

182

152

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Here we address whether FATP2 is 1) a peroxisomal enzyme, 2) a plasma membrane-associated long-chain fatty acid (LCFA) transporter, or 3) a multifunctional protein. We found that, in mouse livers, only a minor fraction of FATP2 localizes to peroxisomes, where it contributes to approximately half of the peroxisomal VLACS activity. However, total hepatic (V)LACS activity was not significantly affected by loss of FATP2, while LCFA uptake was reduced by 40%, indicating a more prominent role in hepatic LCFA uptake. This suggests FATP2 as a potential target for a therapeutic intervention of hepatosteatosis. Adeno-associated virus 8-based short hairpin RNA expression vectors were used to achieve liver-specific FATP2 knockdown, which significantly reduced hepatosteatosis in the face of continued high-fat feeding, concomitant with improvements in liver physiology, fasting glucose, and insulin levels. Based on our findings, we propose a model in which FATP2 is a multifunctional protein that shows subcellular localization-dependent activity and is a major contributor to peroxisomal

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Section: Resultsmentioning

confidence: 90%

FATP2 is a hepatic fatty acid transporter and peroxisomal very long-chain acyl-CoA synthetase

Falcon

Doege

Fluitt

et al. 2010

American Journal of Physiology-Endocrinology and Metabolism

182

152

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“…We first studied immunostaining of peroxisomal enzymes in mouse liver, such as urate oxidase [98,100] and catalase [99], using cryo-sections and ferritin-antibody oxidase conjugates, together with conven- Fig. 3.…”

Section: Gold Particles In Colloidal Gold Immunostaining (Au)mentioning

confidence: 99%

The Utility Value of High Voltage Electron Microscopy for X-Ray Microanalysis

Nagata

2003

Acta Histochem. Cytochem

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X-ray microanalysis is a useful technique to qualify and quantify basic elements in biological specimens. This article reviews the principles and techniques for applying intermediate high voltage electron microscopy to Xray microanalysis of various elements in biological specimens developed in our laboratory since the late 20th century. We first quantified the endproducts of histochemical reactions such as Ag in radioautographs, Ce in acid phosphatase reaction and Au in colloidal gold immunostaining using semi-thin sections by high voltage electron microscopy at 300-400 kV. We then analyzed various trace elements such as Zn, Ca, and S, which originally existed in the cytoplasmic matrix or cell organelles of various cells in different organs, and some absorbed elements introduced by experimental administration into cells and tissues such as Al, using both conventional chemical fixation and cryofixation followed by cryo-sectioning, freeze-drying, or freeze-substitution. As a result, we showed that the peak to background (P/B) ratios of all the elements analyzed had high P/B ratios at 300-400 kV. It was concluded that X-ray microanalysis using semi-thin sections by high voltage electron microscopy is of great utility for quantifying trace elements in biological specimens.

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“…Since around 2,100 enzymes were registered by the Enzyme Committee of the International Union of Biochemistry (1979), the number of enzymes demonstrable cytochemically is too small. Among them, we developed and studied several enzymes such as cytochrome oxidase (Nagata, 1956), urate oxidase (Yokota & Nagata, 1973, catalase (Yokota & Nagata, 1974), acid and alkaline phosphatase, non-specific esterase (Nagata & Murata, 1980), arylsulfatase (Murata et al, 1975), lipase (Fig. 5) and phospholipase (Nagata & Iwadare, 1984).…”

Section: Color Reactions For Light Microscopymentioning

confidence: 99%

“…Among of these techniques we developed and applied several methods for both macromolecules, PEI for glycoproteins and PA-TCH-SP for glucides (Nagata, 2000c), as well as enzymes, such as lipase ) (Fig. 1), phospholipase (Nagata & Iwadare, 1984), catalase (Yokota & Nagata, 1974) and urate oxidase (Yokota & Nagata, 1973.…”

Section: Electron Dense Deposits For Electron Microscopymentioning

confidence: 99%

<b>Histochemistry, General and Special</b>

Nagata

2008

Annu Rev Biomed Sci

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Histochemistry has been developed between the morphology and functionology, employing both anatomy and biochemistry to fill up the gap between both. Histochemistry localizes chemical components of cells and tissues on histological sections by using various techniques. This field was first established by developing techniques for demonstrating phosphatase activity in 1930's. I had aimed at studying histochemistry by developing new techniques using various principles since 1950's. I had formerly proposed to classify these methods into 3 categories, i.e., chemical, physical and biological techniques. The histochemical techniques have been well developed and systematized by the end of the 20 th century to demonstrate various components such as enzymes, proteins, nucleic acids, glucides, lipids, etc. As a result, many text books are now available dealing with the methodology, which has been well developed in the late 20 th century to form a new scientific field which should be designated as "General Histochemistry". These techniques should be applied to all the organ systems of men and animals as applications of histochemistry to special histology. The results of these applications to all the organs should be collected to form a new field, i.e., "Histochemistry of the Organs" like the histology of the organs or special histology. These results should form a new field in medical sciences, which can be designated as "Special Histochemistry" developing to a part of the microscopic anatomy together with the special histology. These data include not only 3-dimensional structure of the organs but also the 4-dimensional structure in connection with the individual aging.

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Ultrastructural localization of catalase on ultracryotomic sections of mouse liver by ferritin-conjugated antibody technique

Cited by 19 publications

References 18 publications

FATP2 is a hepatic fatty acid transporter and peroxisomal very long-chain acyl-CoA synthetase

FATP2 is a hepatic fatty acid transporter and peroxisomal very long-chain acyl-CoA synthetase

The Utility Value of High Voltage Electron Microscopy for X-Ray Microanalysis

<b>Histochemistry, General and Special</b>

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