Ultrastructural localization of glutathione S-transferase-pi in human colorectal cancer cells

Guo, Wen Jun

doi:10.3748/wjg.v6.i3.454

Cited by 9 publications

(2 citation statements)

References 3 publications

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“…The authors suggest that the characteristic nuclear expression of GST-pi can be a significant marker in colon and rectum cancers. 21 Our results also demonstrate GST-pi overexpression in the nuclei of LoVo cells immediately after PDR. After longer incubation times the nuclear enzyme expression was not detectable.…”

Section: Discussionsupporting

confidence: 66%

Oxidative alterations inducedin vitroby the photodynamic reaction in doxorubicin-sensitive (LoVo) and -resistant (LoVoDX) colon adenocarcinoma cells

Kulbacka¹,

Chwiłkowska²,

Bär³

et al. 2010

Exp Biol Med (Maywood)

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In photodynamic therapy (PDT) a tumor-selective photosensitizer is administered and then activated by exposure to a light source of appropriate wavelength. Multidrug resistance (MDR) is largely caused by the drug efflux from the tumor cell by means of P-glycoprotein, resulting in reduced efficacy of the anticancer therapy. This study deals with photodynamic therapy with Photofrin (Ph) on colon cancer cell lines (doxorubicin-sensitive and -resistant). The cells were treated with 15 and 30 microg/mL Ph and then irradiated by a light dose of 3 or 6 J/cm(2) (632.8 nm). After irradiation the cells were incubated for 0, 3 or 18 h. Crucial factors of oxidative stress (thiobarbituric acid reactive substances [TBARS], protein damage, thiazolyl blue tetrazolium bromide [MTT] assay), changes in cytosolic superoxide dismutase (SOD1) activity after photodynamic reaction (PDR), and the intracellular accumulation of photosensitizers in the cells were examined. Moreover, the expressions of glutathione S-transferase (GST)-pi, a marker protein for photochemical toxicity, and secretory phospholipase A(2), a prognostic and diagnostic marker for colon cancers, were determined. After PDR, increases in SOD1 activity and the level of TBARS were observed in both cell lines. The level of protein-associated -SH groups decreased after PDR. Both cell lines demonstrated stronger GST-pi and PLA(2) expression after PDR, especially after 18 h of incubation. The increasing level of reactive oxygen species following the oxidation of sulfhydryl cell groups and lipid peroxidation influence the activity of many transporters and enzymes. The changes in SOD1 activity show that photodynamic action generates oxidative stress in treated cells. Our study presents that PDR caused oxidative alterations in both examined colon adenocarcinoma cell lines. However, the MDR cells reacted more slowly and all oxidative changes occurred in the delay.

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Section: Discussionsupporting

confidence: 66%

Oxidative alterations inducedin vitroby the photodynamic reaction in doxorubicin-sensitive (LoVo) and -resistant (LoVoDX) colon adenocarcinoma cells

Kulbacka¹,

Chwiłkowska²,

Bär³

et al. 2010

Exp Biol Med (Maywood)

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“…regarding the localization of the GST-P enzyme, Guo et al (Guo et al, 2000) found that it is located in the cytoplasm, mitochondria, lysosomes and nucleus adjacent to the nuclear membrane of colorectal cancer cells. Intracytoplasmic and intranuclear presence of GST-P has also been detected in bladder carcinoma (Chen et al, 1998), although the latter has not been shown previously in liver cancer.…”

Section: Acknowledgementsmentioning

confidence: 99%

Potential effect of Nigella sativa against Diethylnitrosamine-induced hepatocarcinogenesis in rats

Ahmed

Brellou

Gameel

et al. 2017

J Hellenic Vet Med Soc

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Objective of the investigation was the study of potential protective effects of the watery extract of Nigella sativa against diethylnitrosamine induced hepatocarcinogenesis in rats. N. sativa was administered to rats for protection against diethylnitrosamine-induced hepatocarcinogenesis. It was administered prior to, simultaneously with or after injection of diethylnitrosamine. Five groups of Wister rats were used. Group A was administered diethylnitrosamine and N. sativa simultaneously, group B was administered only diethylnitrosamine and group C received only N. sativa. These three groups were maintained for up to eight weeks. Group D received N. sativa six weeks after administration ofdiethylnitrosamine,while group E (“protective group”) received N. sativa on day 1 and diethylnitrosamine six weeks later. These two groups were maintained for up to 12 weeks. All rats were subjected to partial hepatectomy to enhance carcinogenesis. P-isoform of glutathione s transferase (GST-P) was detected in the cytoplasm and nuclei of hepatocytes. The number of GST-P positive foci was significantly smaller in test groups (A, D, E), particularly in groups A and E, when compared with to those in group B, indicating that N. sativa has protective effects against diethylnitrosamine induced liver cancer in rats, even in the very early stages of hepatocarcinogenesis.

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Expression of the xenobiotic- and reactive oxygen species-detoxifying enzymes, GST-pi, Cu/Zn-SOD, and Mn-SOD in the endocrine cells of colorectal cancer

Gulubova

Vlaykova

2010

Int J Colorectal Dis

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Most of the ChGA-positive endocrine cells in colorectal cancers also expressed some or all of the three studied antioxidant enzymes, GST-pi, SOD1, and SOD2. Moreover, patients having tumors with GST-pi-positive endocrine cells have an unfavorable prognosis. We suggest that not the neuroendocrine differentiation in general, but the presence in the tumors of endocrine cells with activated antioxidant defense and probably metabolically more active might determine a more aggressive type of cancer leading to worse prognosis for patients.

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Ultrastructural localization of glutathione S-transferase-pi in human colorectal cancer cells

Cited by 9 publications

References 3 publications

Oxidative alterations inducedin vitroby the photodynamic reaction in doxorubicin-sensitive (LoVo) and -resistant (LoVoDX) colon adenocarcinoma cells

Oxidative alterations inducedin vitroby the photodynamic reaction in doxorubicin-sensitive (LoVo) and -resistant (LoVoDX) colon adenocarcinoma cells

Potential effect of Nigella sativa against Diethylnitrosamine-induced hepatocarcinogenesis in rats

Expression of the xenobiotic- and reactive oxygen species-detoxifying enzymes, GST-pi, Cu/Zn-SOD, and Mn-SOD in the endocrine cells of colorectal cancer

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