Ultrastructural localization of lectin receptors in the preimplantation ovine embryo

Paz, P. de; Sánchez, Alejandra; Fernández, J. García; Garcia, C.; Chamorro, C.A.; Anel, L.

doi:10.1002/ar.1092400411

Cited by 5 publications

(1 citation statement)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Extensive works on the binding of lectins to the cell surfaces have been carried out. Studies on the alterations of lectin binding sites during testicular (Kurohmaru et al 1995, Wine andChapin 1997) and embryonic (Depaz et al 1994, Darmico and Jacobs 1995, Miosge et al 1997) development have been reported. Nevertheless, little work has been done on differentiation of the carbohydrate moieties during oocyte development (Fang and Welsh 1995).…”

mentioning

confidence: 99%

Differentiation of Lectin Binding Patterns in Developing Oocytes of the Giant Freshwater Prawn, Macrobrachium rosenbergii.

Damrongphol

Poolsanguan

2000

CYTOLOGIA

View full text Add to dashboard Cite

Summary Five different peroxidase-labeled lectins, Helix pomatia agglutinin (HPA), Concanavalin A agglutinin (Con A), Ricinus communis agglutinin (RCA-I), Wheat germ agglutinin (WGA) and Ulex europaeus agglutinin (UEA-I), were used to investigate alterations of the lectin binding patterns during oocyte development of the giant freshwater prawn, Macrobrachium rosenbergii. HPA, Con A and RCA-I binding sites were detected in the cytoplasm of developing oocytes; whereas HPA, WGA and UEA-I binding sites were localized on the oocyte surfaces. Although, HPA binding to the cytoplasm of developing oocytes could be detected at all stages of development, significant differentiation of the binding occurred at vitellogenic stage. Con A or RCA-I binding to the cytoplasm was first detected at early vitellogenic stage but the patterns of binding were altered at later stage. Moreover, HPA, Con A or RCA-I was reactive to different regions of the cytoplasm and produced different types of the reaction products. Formation of new surface components at vitellogenic stage was also implicated. The oocyte surfaces began to be reactive to HPA at early vitellogenic stage and were intensely reactive to HPA, WGA and UEA-I at late vitellogenic stage. Furthermore, affinity of HPA or RCA-I to the nucleolus was also detected at late vitellogenic stage.

show abstract

mentioning

confidence: 99%

Differentiation of Lectin Binding Patterns in Developing Oocytes of the Giant Freshwater Prawn, Macrobrachium rosenbergii.

Damrongphol

Poolsanguan

2000

CYTOLOGIA

View full text Add to dashboard Cite

show abstract

Differential expression and regulation of PNA and UEA‐1 bindings in rabbit uterus during preimplantation period

Guo

Duan

Wang

et al. 2013

Microscopy Res & Technique

View full text Add to dashboard Cite

Peanut agglutinin (PNA) and Ulex europaeus agglutinin-1 (UEA-1) were used as probes to study the distribution of β-gal (1→3) ga1Nac and α-L-Fucose in rabbit uterus during early pregnancy. PNA binding was mainly localized on the surface of uterine glandular and luminal epithelium. There were no positive signals on day 1 of pregnancy. PNA binding gradually increased from day 2 and reached its highest level on days 3 and 4. The distribution of PNA binding gradually declined from day 5 and reached a low level on day 7. However, UEA-1 binding was only localized on the luminal epithelial during early pregnancy. A high level of UEA-1 binding had been found on the luminal epithelium on day 1 of pregnancy and low level of positive signals had been found in the uterus on days 2 and 3. UEA-1 binding increased gradually and reached its highest level on day 4. Then the distribution of UEA-1 binding sharply declined and no positive signals were found on days 5-7. The distribution of PNA and UEA-1 bindings in pseudopregnant uterus was similar to that in normal pregnant uterus. During estrus cycle, there was no detectable PNA binding signal in uterus. But, a high level of UEA-1 binding was found in the luminal epithelium of estrus uterus. In ovariectomized rabbit uterus, progesterone significantly induced the expression of PNA binding, while estrogen stimulated UEA-1 binding expression. These results suggested that the distribution of PNA and UEA-1 bindings in rabbit uterus may be related to rabbit implantation.

show abstract

Early and natural embryonic death in Lagostomus maximus: Association with the uterine glands, vasculature, and musculature

Acuña

Barbeito

Portiansky

et al. 2020

Journal of Morphology

View full text Add to dashboard Cite

The uterus is an organ with great plasticity due to the morphological and physiological changes it experiences during the estrous cycle and pregnancy. In mammals, pregnancy requires diverse sex hormones, growth factors and cytokines, among others, for promoting uterine remodeling to favor implantation, placentation, and embryo/fetus survival and growth. The hystricognathi rodent Lagostomus maximus (plains viscacha) has a high rate of embryonic resorption. The cranial and middle implants are reabsorbed 25–35 days after intercourse while the caudal embryos continue with their development until two precocial offspring are born. So far, no uterine studies of non‐pregnant L. maximus females were performed to determine the possible existence of variations in the organ that could be related to the differential survival of the implants. We used ultrasonography, as well as morphological, morphometric, histochemical, lectinhistochemical, and immunohistochemical methods to study differences in the uterine glands (area), vasculature (area), and musculature (thickness) along the uterine horns in non‐pregnant females. Along the uterus, all these structures were in more advanced developmental condition in the caudal region as compared to more anterior positions. These regional variations could be decisive in explaining the reason why only caudal implantations come to term. In contrast, no differences in the in the luminal and glandular epithelial cells, nor in the degree of cell proliferation and apoptosis, and hormonal receptor staining were found. These parameters could be related to implantation along the uterine horns, but not to the differential survival of the implants.

show abstract

Ultrastructural localization of lectin receptors in the preimplantation ovine embryo

Cited by 5 publications

References 28 publications

Differentiation of Lectin Binding Patterns in Developing Oocytes of the Giant Freshwater Prawn, Macrobrachium rosenbergii.

Differentiation of Lectin Binding Patterns in Developing Oocytes of the Giant Freshwater Prawn, Macrobrachium rosenbergii.

Differential expression and regulation of PNA and UEA‐1 bindings in rabbit uterus during preimplantation period

Early and natural embryonic death in Lagostomus maximus: Association with the uterine glands, vasculature, and musculature

Contact Info

Product

Resources

About