Ultrastructural localization of osteopontin in the kidney

Madsen, Kirsten; Zhang, L; Shamat, A R Abu; Siegfried, Sari; Ma, J H

doi:10.1681/asn.v871043

Cited by 38 publications

(2 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Osteopontin is another marker of AKI [ 65 ]. This was observed in tubular epithelial cells, suggesting that the expression of this protein could be changed during tubular damage [ 66 , 67 ]. Indeed, increased expression of osteopontin was demonstrated in the kidney epithelial cells of small mammal models of hypoxia-induced kidney damage [ 68 ], polycystic kidney disease [ 69 ], and angiotensin II-induced tubulointerstitial nephritis [ 70 ].…”

Section: Discussionmentioning

confidence: 99%

Rosuvastatin as a Supplemental Treatment for the Clinical Symptoms of Nephropathia Epidemica: A Pilot Clinical Study

Shakirova,

Markelova,

Davidyuk

et al. 2024

Viruses

View full text Add to dashboard Cite

Nephropathis epidemica (NE), a mild form of hemorrhagic fever with renal syndrome (HFRS), is an acute zoonotic disease endemic in the Republic of Tatarstan. This study aimed to assess the impact of rosuvastatin on the clinical and laboratory results of NE. A total of 61 NE patients and 30 controls were included in this study; 22 NE patients and 7 controls received a daily dose of rosuvastatin (10 mg) for ten consecutive days. Serum samples were collected on days 1, 5, and 10 after admission to the hospital. These samples were analyzed to determine the levels of lipids, cytokines, and kidney toxicity markers. Our findings indicate that rosuvastatin reduced the duration of the second wave of fever and alleviated back pain and headache symptoms. Additionally, low-density lipoprotein cholesterol (LDL-C) serum levels were significantly decreased on days 5 and 10 upon rosuvastatin treatment. Furthermore, rosuvastatin decreased the levels of cytokines in the serum, particularly proinflammatory cytokines IL-1β and IL-8. NE patients had significantly altered levels of the kidney toxicity markers albumin and osteopontin. The data from our study provide evidence supporting the therapeutic potential of rosuvastatin in NE cases.

show abstract

Section: Discussionmentioning

confidence: 99%

Rosuvastatin as a Supplemental Treatment for the Clinical Symptoms of Nephropathia Epidemica: A Pilot Clinical Study

Shakirova,

Markelova,

Davidyuk

et al. 2024

Viruses

View full text Add to dashboard Cite

show abstract

“…Osteopontin is enriched in the renal tubular epithelium, ureteric buds and certain interstitial cells of the foetal human kidney (Hudkins et al, 1999). In the developing mouse kidney, Osteopontin expression was detected in the loop of Henle and localized in the thin limb and papillary surface epithelium (Madsen et al, 1997), distal tubules (Kohri et al, 1993), parietal epithelial cells and collecting duct (Pichler et al, 1995) but not in the proximal tubules (Hudkins et al, 1999). Osteopontin is an important factor in tubulogenesis (Hara et al, 1999), cell regeneration (Xie et al, 2001) and cell apoptosis (Rangaswami et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Differential expression of epithelial and smooth muscle lineage‐specific markers of metanephros in one‐humped camel foetuses

Farouk,

Basha,

Emam

et al. 2023

Anat Histol Embryol

View full text Add to dashboard Cite

The development of the metanephros in one‐humped camels involves a complex series of interactions between epithelial and mesenchymal cells. As a result, there is a synchronized differentiation process of stromal, vascular and epithelial cell types during glomerulogenesis, angiogenesis and tubulogenesis. In the current work, the metanephros of camel foetuses were divided into four stages where kidneys from each stage were processed and immunoassayed, followed by quantitative analysis to determine target protein intensities throughout metanephrogenesis in the camel. This study demonstrated robust expression of α‐smooth muscle actin (α‐SMA) in the glomerular mesangium, as well as in interlobular and glomerular arterioles during the earlier stages of development. However, in the late stages, α‐SMA expression became more localized around the blood capillaries in both the cortex and medulla. Strong expression of CD34 was observed in the immature glomerular and peritubular endothelial cells within the subcapsular zone, as well as in the glomerular, proximal tubular and distal tubular epithelium of stage one foetuses, although its expression gradually diminished with foetal maturation. The expression pattern of osteopontin was prominently observed in the distal convoluted tubules throughout all stages, however, no expression was detected in the proximal tubules, glomeruli and arterioles. E‐cadherin was detected in the developing renal tubular epithelial cells but not in the glomeruli. In conclusion, this study reveals the spatiotemporal distribution of key proteins, including α‐SMA, CD34, Osteopontin and E‐cadherin, which play a crucial role in metanephrogenesis in camel foetuses.

show abstract

Evaluation of antiurolithic effect and the possible mechanisms of Desmodium styracifolium and Pyrrosiae petiolosa in rats

Duan

Zhang

et al. 2011

Urol Res

View full text Add to dashboard Cite

Chinese herbs Desmodium styracifolium (Ds) and Pyrrosiae petiolosa (Pp) have been widely used to treat urolithiasis with few side effects in traditional Chinese medicine (TCM). In vitro crystallization study has confirmed their prophylaxis of Calcium oxalate (CaO( x )) stones formation. However, little is known on their possible mechanisms in vivo. In the present study, we proposed to systematically evaluate their antilithic effects and clarify the underlying mechanism of Ds and Pp using a rat nephrolithiasis model and administering the aqueous extracts. Adult male Wistar rats were fed with 5% ammonium oxalate (AmO( x )) forage to induce nephrolithiasis. After 1 week, the rats were randomly divided into eight groups, and given low, medium, and high dose of each herb treatments (275, 550 and 1,100 mg/kg for Ds and 150, 300 and 600 mg/kg for Pp) by gavage for 3 weeks. In contrast to urolithic rats, urinary oxalate significantly reduced, and urinary calcium increased in medium and high dose Ds groups. Few depositions were observed in kidney with slight dilation of tubules and inflammatory infiltration. Osteopontin (OPN), nitric oxide (NO), and malondialdehyde (MDA) levels significantly decreased, but Superoxide dismutase (SOD) activities were enhanced in kidneys. In high dose Pp group, crystals were found in the dilation of tubules with slight inflammatory infiltration. Partial serum and urinary variables returned to the normal range. In conclusion, medium and high dose Ds has beneficial effect in preventing CaO( x ) stone formation by rising urinary Citrate excretion, decreasing urinary calcium, diuresis, and antioxidative effects. Although high dose Pp has relatively weak prophylaxis, it indicates significant anti-inflammatory and antioxidant effects.

show abstract

Ultrastructural localization of osteopontin in the kidney

Cited by 38 publications

References 29 publications

Rosuvastatin as a Supplemental Treatment for the Clinical Symptoms of Nephropathia Epidemica: A Pilot Clinical Study

Rosuvastatin as a Supplemental Treatment for the Clinical Symptoms of Nephropathia Epidemica: A Pilot Clinical Study

Differential expression of epithelial and smooth muscle lineage‐specific markers of metanephros in one‐humped camel foetuses

Evaluation of antiurolithic effect and the possible mechanisms of Desmodium styracifolium and Pyrrosiae petiolosa in rats

Contact Info

Product

Resources

About