Ultrastructural localization of peroxidase activity in neutrophil leukocytes of Ictalurus punctatus

Abstract

The cytoplasmic granules of the blood neutrophil leukocyte of the teleost, Ictalurus punctatus, have been shown to exhibit peroxidase activity at the light and electron microscopic levels when exposed to the 3,3′-diaminobenzidine tetrahydrochloride peroxidase procedure. Erythrocytes also show activity. The addition of cyanide to the incubation medium has no apparent effect on peroxidase reactivity in neutrophils, but inhibits pseudoperoxidase reactivity in erythrocytes. The presence of peroxidase-positive granules in the neutrophil serve as a marker for identification of this cell and strongly indicate antibacterial and phagocytic functions for the neutrophil.

An in vitroassay to measure phagocytosis in striped bass hybrids (Morone saxatilis × Morone chrysops)

“…The strong peroxidase activity observed in neutrophils of this hybrid was consistent with reports on neutrophil peroxidase reaction in striped bass, Morone saxatilis (Bodammer et al, 1990), channel catfish, Ictalurus punctatus (Cannon et al, 1980), plaice Pleuronectes platessa L. (Ellis, 1976) and rainbow trout, Oncorhynchus mykiss (Ainsworth, 1992). The negative peroxidase reaction demonstrated by the macrophages in this study is also similar to the findings of Zeliko# & Enane (1991) in rainbow trout and Calduch-Giner et al (1997) in gilthead seabream, Sparatus aurata.…”

Ultrastructural observations on the phagocytic behavior of winter flounder Pleuronectes americanus peritoneal neutrophils and macrophages in vivo

“…The presence of peroxidase in the bacteria-containing phagosomes of winter flounder neutrophils provides supportive evidence that these cells have microbicidal capability via the H202-peroxidase-halide system thought to be operant in the neutrophils of other fish species (Nash et al 1987, Ainsworth 1992. Peroxidase positive granules have also been observed in both developing and mature neutrophils in plaice (Ellis 1976) and in other species of teleosts (Cannon et al 1980a, Bielek 1981, Suzuki 1984, Bayne 1986, Hine et a!. 1986, Doggett et al 1987, Finco-Kect h Thune 1987, Bodammer et al 1990).…”

Cellular responses in the skin of the trout (Oncorhynchus mykiss) exposed to temperature elevation

“…Previously we reported this enzyme in basophilic granulocytes of carp (Iger & Abraham, 1990). It was also observed in neutrophils of Ictalurus punctatus Rafinesque (Cannon et al, 1980). This enzyme was further detected in epidermal cells which contained phagosomes (Iger & Abraham.…”